Separation and identification of antibacterial chamomile components using OPLC, bioautography and GC-MS

Components of 50% ethanolic chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line OPLC, which consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected peaks were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% ethanolic extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone

In-situ Clean-up and OPLC Fractionation of Chamomile Flower Extract Searching Active Components by Bioautography

Bioassay-guided isolation of antibacterial components of chamomile flower methanol extract was performed by OPLC with on-line detection, fractionation combined with sample clean-up in-situ in the adsorbent bed after sample application. The antibacterial effect of the fractions and the separated compounds remained on the adsorbent layer (do not overrun during OPLC separation) was tested with direct bioautography (DB) against the bioluminescent Pseudomonas savastanoi pv. maculicola and Vibrio fischeri. The fractions with great biologically activity were analysed by SPME-GC-MS and LC-MS/MS and the two active uneluted compounds were characterized by OPLC-MS using interface. Mainly essential oil components, coumarins, flavonoids, phenolic acids and fatty acids were identified in the fractions

Role of direct bioautographic method for detection of antistaphylococcal activity of essential oils.

The aim of the present study was the chemical characterization of some traditionally used and therapeutically relevant essential oils (thyme, eucalyptus, cinnamon bark, clove, and tea tree) and the optimized microbiological investigation of the effect of these oils on clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-susceptible S. aureus (MSSA). The chemical composition of the oils was analyzed by TLC, and controlled by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The antibacterial effect was investigated using a TLC-bioautographic method. Antibacterial activity of thyme, clove and cinnamon oils, as well as their main components (thymol, carvacrol, eugenol, and cinnamic aldehyde) was observed against all the bacterial strains used in this study. The essential oils of eucalyptus and tea tree showed weak activity in the bioautographic system. On the whole, the antibacterial activity of the essential oils could be related to their most abundant components, but the effect of the minor components should also be taken into consideration. Direct bioautography is more cost-effective and better in comparison with traditional microbiological laboratory methods (e.g. disc-diffusion, agar-plate technique)

Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

The isolation and characterization of antibacterial chamomile components were performed by the use of direct bioautography and solid phase microextraction (SPME)-GC-MS. Four ingredients, active against Vibrio fischeri, were identified as the polyacetylene geometric isomers cis- and trans-spiroethers, the coumarin related herniarin, and the sesquiterpene alcohol (-)-alpha-bisabolol

Studies and results concerning a new plant containing essential oil [(Porophyllum ruderale (DC.) R. R. Johnson (Asteraceae)]

vokMTT mikkel

Studies on the determination of the essential oil composition of Artemisia species by means of GC, GC/MS methods (Finnish-Hungarian scientific cooperation)

vokEKO/Karil

Crop yield and essential oil content of the Syrian marjoram, Za'atar (Origanum syriacum L.) in South-Finland

vo

The wintering and the essential oil content of rock-savory (Micromeria thymifolia) (Scop.) Fritisch, in South-Finland

vokMTT Mikkel

Determination of the essential oil composition of Ligusticum scoticum L. by means of GC, GC/MS methods

vokEKO/Karil