Control of expression of the RNases J1 and J2 in bacillus subtilis

In Bacillus subtilis, the dual activity 5' exo- and endoribonucleases J1 and J2 are important players in mRNA and stable RNA maturation and degradation. Recent work has improved our understanding of their structure and mechanism of action and identified numerous RNA substrates. However, almost nothing is known about the expression of these enzymes. Here, we have identified the transcriptional and translational signals that control the expression of the rnjA (RNase J1) and rnjB (RNase J2) genes. While the rnjB gene is transcribed constitutively from a sigma A promoter, optimal expression of RNase J1 requires cotranscription and cotranslation with the upstream ykzG gene, encoding a protein of unknown function. In the absence of coupled translation, RNase J1 expression is decreased more than 5-fold. Transcription of the ykzG operon initiates at a sigma A promoter with a noncanonical -35 box that is required for optimal transcription. Biosynthesis of RNase J1 is autocontrolled within a small range (1.4-fold) and also slightly stimulated (1.4-fold) in the absence of RNase J2. These controls are weak but might be useful to maintain the overall RNase J level and possibly also equimolar amounts of the two nucleases in the cell that primarily act as a heterodimer in vivo. © 2014, American Society for Microbiology

The Federal Administrative Court Proposal: An Examination of General Principals

Simulations of relativistic hydrodynamics often need both high accuracy and robust shock-handling properties. The discontinuous Galerkin method combines these features—a high order of convergence in regions where the solution is smooth and shock-capturing properties for regions where it is not—with geometric flexibility and is therefore well suited to solve the partial differential equations describing astrophysical scenarios. We present here evolutions of a general-relativistic neutron star with the discontinuous Galerkin method. In these simulations, we simultaneously evolve the spacetime geometry and the matter on the same computational grid, which we conform to the spherical geometry of the problem. To verify the correctness of our implementation, we perform standard convergence and shock tests. We then show results for evolving, in three dimensions, a Kerr black hole; a neutron star in the Cowling approximation (holding the spacetime metric fixed); and, finally, a neutron star where the spacetime and matter are both dynamical. The evolutions show long-term stability, good accuracy, and an improved rate of convergence versus a comparable-resolution finite-volume method

Thermal phase diagrams of columnar liquid crystals

In order to understand the possible sequence of transitions from the disordered columnar phase to the helical phase in hexa(hexylthio)triphenylene (HHTT), we study a three-dimensional planar model with octupolar interactions inscribed on a triangular lattice of columns. We obtain thermal phase diagrams using a mean-field approximation and Monte Carlo simulations. These two approaches give similar results, namely, in the quasi one-dimensional regime, as the temperature is lowered, the columns order with a linear polarization, whereas helical phases develop at lower temperatures. The helicity patterns of the helical phases are determined by the exact nature of the frustration in the system, itself related to the octupolar nature of the molecules.Comment: 12 pages, 9 figures, ReVTe

Transport and magnetic properties in YBaCo2O5.45: Focus on the high-temperature transition

The electronic transport properties and the magnetic susceptibility were measured in detail in $YBaCo_2O_{5.45}$. Close to the so-called metal-insulator transition, strong effects of resistance relaxation, a clear thermal hysteresis and a sudden increase of the resistance noise are observed. This is likely due to the first order character of the transition and to the underlying phases coexistence. Despite these out of equilibrium features, a positive and linear magneto-resistance is also observed, possibly linked to the heterogeneity of the state. From a magnetic point of view, the paramagnetic to ordered magnetic state transition is observed using non linear susceptibilty. This transition shows the characteristics of a continuous transition, and time dependent effects can be linked with the dynamics of magnetic domains in presence of disorder. Thus, when focusing on the order of the transitions, the electronic one and the magnetic one can not be directly associated.Comment: accepted for publication in PR

Supersolid phases in the one dimensional extended soft core Bosonic Hubbard model

We present results of Quantum Monte Carlo simulations for the soft core extended bosonic Hubbard model in one dimension exhibiting the presence of supersolid phases similar to those recently found in two dimensions. We find that in one and two dimensions, the insulator-supersolid transition has dynamic critical exponent z=2 whereas the first order insulator-superfluid transition in two dimensions is replaced by a continuous transition with z=1 in one dimension. We present evidence that this transition is in the Kosterlitz-Thouless universality class and discuss the mechanism behind this difference. The simultaneous presence of two types of quasi long range order results in two soliton-like dips in the excitation spectrum.Comment: 4 pages, 5 figure

Rhodium Doped Manganites : Ferromagnetism and Metallicity

The possibility to induce ferromagnetism and insulator to metal transitions in small A site cation manganites Ln_{1-x}Ca_xMnO_3 by rhodium doping is shown for the first time. Colossal magnetoresistance (CMR) properties are evidenced for a large compositional range (0.35 \leq x < 0.60). The ability of rhodium to induce such properties is compared to the results obtained by chromium and ruthenium doping. Models are proposed to explain this behavior.Comment: 11 pages, 8 figure

Strategy to design the sea-level monitoring networks for small tsunamigenic oceanic basins: the Western Mediterranean case

The 26 December 2004 Indian Ocean tsunami triggered a number of international and national initiatives aimed at establishing modern, reliable and robust tsunami warning systems. In addition to the seismic network for initial warning, the main component of the monitoring system is the sea level network. Networks of coastal tide gages and tsunameters are implemented to detect the tsunami after the occurrence of a large earthquake, to confirm or refute the tsunami occurrence. Large oceans tsunami monitoring currently in place in the Pacific and in implementation in the Indian Ocean will be able to detect tsunamis in 1 h. But due to the very short time of waves propagation, in general less than 1 h, a tsunami monitoring system in a smaller basin requires a denser network located close to the seismic zones. A methodology is proposed based on the modeling of tsunami travel time and waveform, and on the estimation of the delay of transmission to design the location and the spacing of the stations. In the case of Western Mediterranean, we demonstrate that a network of around 17 coastal tide gages and 13 tsunameters located at 50 km along the shore is required to detect and measure nearly all tsunamis generated on the Northern coasts of Africa

What does a binary black hole merger look like?

We present a method of calculating the strong-field gravitational lensing caused by many analytic and numerical spacetimes. We use this procedure to calculate the distortion caused by isolated black holes and by numerically evolved black hole binaries. We produce both demonstrative images illustrating details of the spatial distortion and realistic images of collections of stars taking both lensing amplification and redshift into account. On large scales the lensing from inspiraling binaries resembles that of single black holes, but on small scales the resulting images show complex and in some cases self-similar structure across different angular scales.Comment: 10 pages, 12 figures. Supplementary images and movies can be found at http://www.black-holes.org/the-science-numerical-relativity/numerical-relativity/gravitational-lensin