First landscape of binding to chromosomes for a domesticated mariner transposase in the human genome: diversity of genomic targets of SETMAR isoforms in two colorectal cell lines

Setmar is a 3-exons gene coding a SET domain fused to a Hsmar1 transposase. Its different transcripts theoretically encode 8 isoforms with SET moieties differently spliced. In vitro, the largest isoform binds specifically to Hsmar1 DNA ends and with no specificity to DNA when it is associated with hPso4. In colon cell lines, we found they bind specifically to two chromosomal targets depending probably on the isoform, Hsmar1 ends and sites with no conserved motifs. We also discovered that the isoforms profile was different between cell lines and patient tissues, suggesting the isoforms encoded by this gene in healthy cells and their functions are currently not investigated

ADENOCARCINOME PROSTATIQUE (CORRELATION ENTRE LES INFORMATIONS OBTENUES PAR LES BIOPSIES PROSTATIQUES ECHOGUIDEES ET L'ETUDE DES PIECES DE PROSTATECTOMIE RADICALE (DES UROLOGIE))

ANGERS-BU Médecine-Pharmacie (490072105) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Expression des récepteurs hormonaux des stéroïdes sexuels dans les cellules C thyroïdiennes pathologiques (étude anatomo-clinique de 40 patients porteurs d'une hyperplasie des cellules C et/ou d'un carcinome médullaire de la thyroïde)

TOURS-BU Médecine (372612103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Rapidly Growing Tumour on the Forearm of an 83-year-old Woman: A Quiz

Quiz + réponseInternational audienc

Lymphome intravasculaire : à propos de deux observations autopsiques et revue de la littérature

International audienceIntravacular large B-cell lymphoma (LIV) is a rare entity individualized in the WHO classification since 2001&nbsp;as a subtype of extranodal diffuse large B-cell lymphoma. We report two autopsic cases of LIV: a 77-year-old woman presenting with fever, dyspnea, antehypophyseal failure and a 54-year-old man presenting with fever, weight-loss, night-sweats and encephalopathy. They died respectively 10&nbsp;and 7&nbsp;months after the beginning of symptoms, without diagnosis. Neither infectious disease nor lymphomatous proliferation had been identified. From these two cases and our literature review, we insist on the importance of histopathological diagnosis on biopsy for this rare pathology which clinical diagnosis remains difficult.Les lymphomes intravasculaires à grandes cellules B (LIV) sont rares et individualisés dans la classification de l’OMS depuis 2001&nbsp;comme un sous-type de lymphome B diffus à grandes cellules extraganglionnairea. Nous rapportons deux cas de LIV de diagnostic autopsique. Il s’agissait d’une femme de 77&nbsp;ans ayant présenté fièvre, dyspnée, insuffisance antéhypophysaire et d’un homme de 54&nbsp;ans ayant présenté fièvre, amaigrissement, sueurs nocturnes et encéphalopathie. Ils sont décédés respectivement dix et sept mois après le début des symptômes, sans diagnostic. Les bilans infectieux étaient négatifs. Aucune prolifération lymphomateuse n’avait été mise en évidence. Évoquer et diagnostiquer cliniquement un LIV est difficile car il s’agit d’une pathologie rare dont les symptômes peuvent être très variables. À partir de ces deux observations et de la revue de la littérature, nous présentons l’intérêt des différentes biopsies dans la mise en évidence de la prolifération lymphomateuse.</p

Two-Step Differential Expression Analysis Reveals a New Set of Genes Involved in Thyroid Oncocytic Tumors

International audienc

High kallikrein-related peptidase 6 in non-small cell lung cancer cells: an indicator of tumour proliferation and poor prognosis.

International audienceThe human kallikrein-related peptidases (KLK) are serine proteases whose concentrations are often abnormal in common human malignancies and contribute to neoplastic progression through multifaceted roles. However, little attention has been paid to their synthesis and involvement in the development and dissemination of lung cancer, the leading cause of cancer mortality worldwide. We have analysed the production of KLK6 in normal lung and tumour tissues from patients with non-small cell lung cancer (NSCLC). KLK6 immunoreactivity was restricted to epithelial cells of the normal bronchi, but most of the cancer samples were moderately or highly immunoreactive, regardless of the histological subtype. In contrast, little or no KLK6 was detected in NSCLC cells. We have developed NSCLC lines expressing wild-type KLK6 in order to investigate the role of KLK6 in lung cancer biology, and analysed its impact on proliferation. Ectopic KLK6 dramatically enhanced NSCLC cell growth and KLK6-producing NSCLC cells had accelerated cell cycles, between the G1 and S phases. This was accompanied by a marked increase in cyclin E and decrease in p21. KLK6 production was also associated with enhanced synthesis of c-Myc, which is known to promote cell-cycle progression. Finally, examination of specimens from patients with NSCLC revealed that KLK6 mRNA is overexpressed in tumour tissue, and high KLK6 concentrations were associated with lower survival rates. We conclude that a high concentration of KLK6 is an indicator of tumour proliferation and an independent predictive factor in NSCLC

Generation of Merkel Cell Polyomavirus (MCV)-Like Particles and Their Application to Detection of MCV Antibodies ▿ †

The genome of a new human polyomavirus, known as Merkel cell polyomavirus (MCV), has recently been reported to be integrated within the cellular DNA of Merkel cell carcinoma (MCC), a rare human skin cancer. To investigate MCV seroprevalence in the general population, we expressed three different MCV VP1 in insect cells using recombinant baculoviruses. Viruslike particles (VLPs) were obtained with only one of the three VP1 genes. High-titer antibodies against VP1 VLPs were detected in mice immunized with MCV VLPs, and limited cross-reactivity was observed with BK polyomavirus (BKV) and lymphotropic polyomavirus (LPV). MCV antibodies were detected in 77% of the general population, with no variations according to age