Effects of addition of exogenous fibrolytic enzymes on digestibility and milk and meat production: A systematic review

El articulo esta publicado en una revista de Open accessExogenous fibrolytic enzymes (EFE) added to the ruminant diet can increase fiber digestibility and production efficiency. A systematic review was conducted to understand the interactions between EFE and diet on digestibility and animal performance. The database included variables from 384 experiments with EFE and 264 controls from 85 papers published since 2000 (classification criteria: 1) type of study (in vitro, in situ, in vivo), 2) type of ruminants (sheep, Buffaloes, goats, beef and dairy cattle), 3) primary EFE activity (Cellulases (Cel) or Xylanases (Xyl)), 4) forage proportion (FP), 5) type of plant (TP: legumes or grasses), 6) number of ingredients in diets, and 7) application time (AT)). In over of 52.85% of cases, EFE improved the degradability of dry matter (DMD), neutral and acid detergent fiber (NDFD and ADFD), in vitro gas production (GP), volatile fatty acids (VFA), the acetate: propionate ratio (A:P ratio), protein and fat milk, milk yield and average daily gain (ADG) (by 7.78–21.85%). Cel improved organic matter degradability (OMD), GP, VFA, milk yield, and milk protein and fat content. EFE in FP≥40% diets enhanced the ADG, and in grass-based diets increased the dry matter intake (DMI). The AT of EFE affected the DMD, NDFD, and ADFD. Significant correlations were found between the improvements of NDFD or ADFD with DMD (r>0.59), milk yield (r=0.64), and ADG (r=0.59). In conclusion, many factors interact with EFE supplementation effects, but EFE consistently enhanced the DMD, NDFD, and ADFD of ruminant diets, which are related to improvements in productive performance

Relationship between Neutral Detergent Fiber and In Vitro Digestibility in Test Crosses of Maize Hybrids

fiber (NDF) and in vitro dry matter disappearance (IVDMD) of test crosses of maize. Design/methodology/approach: Crosses of early, intermediate, and late maize lines (total=75) from high valleys and subtropical/tropical testers were sowed in three regions of Central-North Mexico. Male and female flowering days (MFD, FFD); days to harvest (Dcor); plant and cob length (PL, CL); forage, corn stover (CS), and cobs humid base (HB) and dry matter (DM) yields; and NDF, acid detergent fiber (ADF), hemicellulose (Hem), crude protein (CP), and IVDMD were analyzed. Results: High valleys lines had more MFD, FFD, Dcor, and PL, therefore better forage, MS, and cob yields. More MDF, FFD, and H were related to better HB and DM yields, and CP content, but also were related to more NDF, ADF, and Hem proportions, and therefore to a less IVDMD (r=0.47 to 0.98); however, crosslines with high cob yields also had high CS yields (r=0.57-0.68). Regression linear models showed that one unit of NDF might reduce 0.49 to 56% the IVDMD (R2=0.59-0.78); additionally, NDF<68% was related to IVDMD>60% (R2=0.63-0.78). Limitations on study/implications: ADF correlated negatively with IVDMD in early lines; NDF composition should also be related to its degradability (NDFD). Findings/conclusions: Maize-breeding might be directed to obtain hybrids with less NDF CS contents to use them in ruminant diets, maintaining the cob yields for human nutrition and resistance to plant lodging.&nbsp

In vitro gas production and digestibility of oat and triticale forage mixtures ensiled with fibrolytic enzymes and inoculants

Objective: To evaluate the fibrolytic enzymes (FE) and acid-lactic bacterial inoculants (ALB) added to 40 d silages with oats and triticale (O:T) on the proportion and composition of neutral detergent fiber (NDF) and its further in vitro gas production (GP) and digestibility of dry matter (IVDMD). Design/methodology/approach: pH, NDF, acid detergen fiber and lignin (ADF, ADL), hemicellulose (Hem), cellulose (Cel), dry matter (DM), and crude protein (PC), along with the PG (Maximum velocity (Vmax), fractional rate (S), Lag), and IVDMD (24h) of silages O:T (forage proportions (FP): 60:40%, 80:20%) treated with EF (control=0, low dose (LD)=075, medium (MD)=1, and high (HD)=1.25 g/kg of forage in humid base (HB)), and ALB (Control=0, LD=0.188, MD=0.25, and HD=0.31 g/kg HB). Variance analysis (ANOVA) included complete randomized designs (CRD) with factorial and divided parcels arrangements, considering fixed (doses, additives, and FP) and random (place/moment of sampling). Results: ALB improved the IVDMD24 of 60:40 and 80:20 O:T silages. FE did not reduce the NDF of 60:40 silages, but LD and MD increased the Hem and CP, and reduced the ADF, ADL, and Cel, that correlated (r) with the improvement of pH pattern, GP, and IVDMD24. Limitations/implications: The differences in the NDF of mixtures of FP could affect the effectiveness of FE and ALB. Findings/conclusions: Although FE and ALB could not reduce the NDF, they would change the proportions of ADF, ADL, Hem, Cel, and CP of silages, that might improve the GP and IVDMD24.Objective: To assess the effects of adding fibrolytic enzymes (FE) or lactic acid bacteria (LAB) inoculants to 40 d silages with oat and triticale (O:T) mixtures on the ratio and composition of neutral detergent fiber (NDF) and its subsequent in vitro gas production (GP) and in vitro dry matter digestibility (IVDMD) at 24 h. Design/Methodology/Approach: Silages elaborated with two O:T ratios (60:40 and 80:20) treated with low (LD), medium (MD), and high (HD) doses of FE (0.75, 1, and 1.25 g/kg forage in wet basis (WB), respectively), and LAB (0.188, 0.25, and 0.31 g/kg WB, respectively). In both cases (FE and LAB), the control had a value of 0. Subsequently, pH, NDF, acid detergent fiber (ADF), acid detergent lignin (ADL), hemicellulose (HEM), cellulose (CEL), dry matter (DM), crude protein (CP), GP parameters, and IVDMD were assessed. GP parameters included maximum velocity (Vmax), fractional rate (S), and lag. Experiments were planned in complete randomized designs (CRD), including factorial and split-plot arrangements. Variance analysis (ANOVA) models included fixed (doses, additives, and FR) and random (place/moment of sampling) effects. Results: LAB improved the IVDMD at 24 h of 60:40 and 80:20 O:T silages. FE did not reduce the NDF of 60:40 silages, but LD and MD increased the HEM and CP, and reduced the ADF, ADL, and CEL; these results are correlated (r) with the improvement of pH pattern, GP, and IVDMD. Study Limitations/Implications: The differences in the NDF of FR mixtures could affect the effectiveness of FE and LAB. Findings/Conclusions: Although FE and LAB did not reduce the NDF, they changed the ratios of ADF, ADL, HEM, CEL, and CP of silages, potentially improving the GP and IVDMD

Digestibilidad in situ de dietas con harina de nopal deshidratado conteniendo un preparado de enzimas fibrolíticas exógenas

Se evaluó el efecto de un preparado de enzimas fibrolíticas exógenas (celulasas y xilanasas) en la degradabilidad in situ de la materia seca (DisMS), fibra detergente neutro (DFDNr) y fibra detergente ácido residual (DFDAr), en dietas altas o bajas en harina de nopal deshidratado. Se aplicaron concentraciones de 0, 1, 2 y 3 g de enzima por kilogramo de materia seca al inicio y 24 horas antes de la degradación in situ. Se determinó la concentración de ácidos grasos volátiles totales y de nitrógeno amoniacal a las 0, 3, 6, 9, 12 y 24 horas después de aplicarse la enzima. No se observaron efectos en DisMS, DFDNr y DFDAr; la aplicación al inicio de la degradación in situ mostró valores más altos que a 24 horas para DisMS y DFDNr, pero fue menor para DFDAr. No se observaron diferencias en las interacciones entre niveles de enzima, tipo de dieta y tiempo de pretratamiento. La aplicación de 1 y 3 g de enzima, en la dieta con bajo contenido de harina de nopal, tuvo efectos en el incremento de los ácidos grasos volátiles totales; para el nitrógeno amoniacal, los mejores resultados ocurrieron con 0 y 1 g de enzima.It was evaluated the effect of a fybrolitic enzyme product (cellulases and xylanases) on in situ digestibility of dry matter (DisMS), residual neutral detergent fiber (DFDNr) and acid detergent fiber (DFDAr), indehydrated ground prickly pear diets with a low or high level. Enzyme concentrations of 0, 1, 2, and 3 g kg-1 of dry matter applied at the beginning (0 hour) and 24 hours before starting in situ digestibility were used. Total volatile fatty acids and ammonia nitrogen were determined at: 0, 3, 6, 9, 12, and 24 hours after the enzyme application.There were no effects on DisMS, DFDNr, and DFDAr. Initial application of enzyme concentrations (0 hour) was higher than 24 hours for DisMS and DFDNr but lower for DFDAr. No differences were observed in interactionsamong enzyme level, diet and application time. Application of 1 and 3 g of the enzyme to the diet with the low level of prickly pear forage had effects on the increasing of volatile fatty acids; for ammonia nitrogen, the best results were obtained using 0 and 1 g of the enzyme.It was evaluated the effect of a fybrolitic enzyme product (cellulases and xylanases) on in situ digestibility of dry matter (DisMS), residual neutral detergent fiber (DFDNr) and acid detergent fiber (DFDAr), in dehydrated ground prickly pear diets with a low or high level. Enzyme concentrations of 0, 1, 2, and 3 g kg-1 of dry matter applied at the beginning (0 hour) and 24 hours before starting in situ digestibility were used. Total volatile fatty acids and ammonia nitrogen were determined at: 0, 3, 6, 9, 12, and 24 hours after the enzyme application. There were no effects on DisMS, DFDNr, and DFDAr. Initial application of enzyme concentrations (0 hour) was higher than 24 hours for DisMS and DFDNr but lower for DFDAr. No differences were observed in interactions among enzyme level, diet and application time. Application of 1 and 3 g of the enzyme to the diet with the low level of prickly pear forage had effects on the increasing of volatile fatty acids; for ammonia nitrogen, the best results were obtained using 0 and 1 g of the enzyme

Potential effects of delphinidin-3-O-sambubioside and cyanidin-3-O-sambubioside of Hibiscus sabdariffa L. on ruminant meat and milk quality

El articulo esta publicado en una revista de Open accessThe objective was to review the potential effects of adding anthocyanin delphinidin-3-O-sambubioside (DOS) and cyanidin-3-O-sambubioside (COS) of HS in animal diets. One hundred and four scientific articles published before 2021 in clinics, pharmacology, nutrition, and animal production were included. The grains/concentrate, metabolic exigency, and caloric stress contribute to increasing the reactive oxygen species (ROS). COS and DOS have antioxidant, antibacterial, antiviral, and anthelmintic activities. In the rumen, anthocyanin might obtain interactions and/or synergisms with substrates, microorganisms, and enzymes which could affect the fiber degradability and decrease potential methane (CH4) emissions; since anthocyanin interferes with ruminal fatty acids biohydrogenation (BH), they can increase the n-3 and n-6 polyunsaturated fatty acids (PUFA), linoleic acid (LA), and conjugated linoleic acid (CLA) in milk and meat, as well as improving their quality. Anthocyanins reduce plasma oxidation and can be deposited in milk and meat, increasing antioxidant activities. Therefore, the reduction of the oxidation of fats and proteins improves shelf-life. Although studies in ruminants are required, COS and DOS act as inhibitors of the angiotensin-converting enzyme (ACEi) and rennin expression, regulating the homeostatic control and possibly the milk yield and body weight. By-products of HS contain polyphenols as calyces with positive effects on the average daily gain and fat meat quality

Evaluation of three forages as a source of fiber in diets of fattening rabbits in Aguascalientes, Mexico

The objective was to evaluate three forages as a source of fiber in fattening rabbit diets. Mesquite pods, oats and alfalfa hay were used in mixed diets. Thirty-six 1-month-old weaned male rabbits were randomly distributed into three treatments (T1, oat diet; T2, mesquite pod diet; T3, alfalfa diet) for 50 days of fattening. Feed intake, daily weight gain, final gain and feed conversion ratio were recorded. At the end of the experiment, the animals were slaughtered to evaluate the carcass performance. Data were statistically evaluated by analysis of variance and comparison of means test. There were no significant differences in the growing period, but in fattening, T1 was higher than T2 and T3 (P<0.05) and the latter higher than T2 in daily weight gain, total weight gain and dry matter digestibility. Feed conversion ratio was the opposite, T1 and T3 were lower than T2. Carcass yield was higher in T1 than T2 and T3 and the latter, higher than T2. No differences were observed among treatments (P> 0.05) in feed intake. A higher digestibility and better productive parameters were obtained with the inclusion of oats and alfalfa hay in the diets, than using mesquite pods; however, the production costs were higher. The limitation of the study is the low level of preference of rabbit meat in Mexico. The mesquite pods represent a viable alternative due to their low cost and availability in semi-arid regions where there are no water resources for forage production. Key words: Productive parameters, oats, mesquiteObjective: To evaluate three forages as a source of fiber in the diets of fattening rabbits. Design/Methodology/Approach: Whole grain diets with forage oat, mesquite pod, and alfalfa were used. Thirty-six weaned male rabbits were randomly distributed into three treatments (T1, forage oat diet; T2, mesquite pod diet; T3, alfalfa diet). Feed consumption, daily weight gain, total weight gain, and feed conversion were recorded. The animals were slaughtered to evaluate carcass yield. The data were statistically evaluated by analysis of variance and Tukey’s test. Results: T1 recorded greater fattening than both T2 and T3 (P<0.05) and the last treatment surpassed T2 in daily weight gain, total weight gain, and feed digestibility. Regarding feed conversion, T1 and T3 had lower results than T2. In carcass yield, T1 was higher than T2 and T3 —which, on its turn, surpassed T2. Finally, no differences were observed in feed consumption between treatments (P> 0.05). There were also no significant differences in growth. Study Limitations/Implications: Mexicans have a low consumption of rabbit meat. The mesquite pod could be a viable alternative due to its low cost and availability in semi-arid areas. Findings/Conclusions: Forage oat recorded the best productive parameters, followed by alfalfa and mesquite pod; however, the latter had a greater economic advantage

Effect of THI, NDF and rumination in milk production by Holstein cows

Objective: The aim of the present study was to evaluate the effect of the THI, NDF and rumination rate (RR) on milk production in Holstein cows in a dairy farm located in Bajío de San José, Jalisco Design/methodology/approach: The temperature-humidity index (THI) is an indicator of the effect of the environmental climate can have on milk production, likewise nutritional content of forage is affected by weather conditions, as the neutral detergent fiber (NDF) is related with rumination activity of cows, increasing THI has shown a direct effect on milk production in cows. HealthyCow 24 ® CSR was used (SCR Engineers Ltd., Netanya, Israel), to monitor rumination, from a total record of 284 cows with 2, 3, and 4 lactacion through august to december 2020 period, analysing NDF content from total mixed ration and monitoring THI. Results: The results showed there was no effect of THI on milk production (p>0.05), despite having reached 76 THI score, instead NDF (p<0.05) and RR (p<0.001) affected milk production, it was not found effect of THI on RR (p>0.05), and NDF had trend (p<0.1). Limitations on study/implications: There were no limitations for this report. Findings/conclusions: According to the results obtained, THI threshold should be reconsidered according to the resistance of productively active cattle on dairy farms.  Objective: The aim of the present study was to evaluate the effect of the temperature-humidity index (THI), the content of neutral detergent fiber (NDF), and rumination rate (RR) on milk production in Holstein cows in a dairy farm located in Bajío de San José, Jalisco, Mexico. Design/methodology/approach: The THI is an indicator of the effect of the environmental climate can have on milk production, and likewise the nutritional content of forage is affected by weather conditions, such as the NDF is related with rumination activity of cows; increasing THI has shown a direct effect on milk production in cows. The HealthyCow 24 ® CSR remote equipment was used (SCR Engineers Ltd., Netanya, Israel), to monitor rumination, from a total registry of 284 cows with 2, 3, and 4 lactations distributed between August and December 2020 period, analyzing the NDF content from total mixed portion and monitoring the THI. Results: The results showed there was no effect of THI on milk production (p>0.05), despite having reached a THI score of 76, while NDF (p<0.05) and RR (p<0.001) affected milk production; an effect of THI on RR (p>0.05) was not found, and the NDF only had a trend (p<0.1). Limitations on study/implications: There were no limitations for the study. Findings/conclusions: According to the results obtained, the THI threshold should be reconsidered according to the resistance of the productively active cattle on dairy farms

Methane in Dairy Farms in Aguascalientes: Corn Silage

Objective: To evaluate the potential methane gas production from corn silages (CS) intended for Holstein cattle in dairy farms in the state of Aguascalientes (Ags), Mexico. Design/Methodology/Approach: Methane (CH4) is one of the greenhouse gases, and worldwide plans and actions are being developed to monitor, control, and reduce their environmental impact. In Mexico, methane emissions from livestock are equivalent to 10.1% of CO2 equivalent are recorded. CS samples were collected from six municipalities in Ags, representing a total of 18 dairy farms. The in vitro gas production technique was used to determine methane gas production, employing a nested mixed model to compare variables between municipalities using residual maximum likelihood method. Results: The average methane production in CS was 29.3 mL/gDM. The Ags municipality showed significantly higher methane production (35.9 mL/gDM, p<0.05), while San Francisco de los Romo (SFR) displayed the lowest production (21.5 mL/gDM, p<0.05). In the state of Aguascalientes, CS-derived CH4 production was projected at approximately 2,884 metric tons (MT) annually. Study Limitations/Implications: There were no identified limitations in the study. Findings/Conclusions: The potential CH4 gas production derived from CS projected in the study represented 0.103% of what was reported by INEGyCEI in 2019

Meta-analysis: effects of exogenous fibrolytic enzymes in ruminant diets

There are unknown interactions between supplements of exogenous fibrolytic enzymes (EFE) and the cell walls of feedstuff in ruminal conditions. The quantitative effects of using EFE in ruminant diets were evaluated using meta-analysis. Records (586) were extracted from 74 journal articles from a list of published papers (2000–2012). Statistical analyses were performed considering fixed [type of forage-based diet, forage-to-concentrate ratio (F:C ratio) and primarily enzyme activities in the EFE], and random effects [Experiment(Article)]. In dairy cows fed high-forage (F:C ≥50%), the supplementation of primarily mixtures of cellulases and xylanases (Cel:Xyl: 1:4–1:1) increased milk production and milk composition of legume-based diets, and primarily xylanases (Xyl) EFE improved those variables of grass-based diets. In F:C <50% grass-based diets, Cel:Xyl improved the average daily gain (ADG) and feed conversion [FC:DM intake (DMI)/ADG] of beef cattle. DMI of dairy cows was not affected by EFE supplementation, but EFE improved the DMI of beef cattle. EFE effects were inconsistent in sheep productive performance variables. Cellulases (Cel) and Xyl enhanced in vivo dry matter (DM) digestibility (DMD) in low-forage (F:C <50%) grass-based diets. In F:C ≥50% legume-based diets, EFE enhanced the in situ DM disappearance (ISDMD), and mainly Cel:Xyl improved the in situ neutral detergent fibre (NDF) disappearance (ISNDFD), but there were no effects in those variables in F:C ≥50% grass-based diets. Regardless of the type of ruminal liquid (RL) or forage, in F:C ≥50% diets, in vitro DM degradability (IVDMD) was improved mainly by Cel, but fibre degradability only was improved by Cel:Xyl when sheep RL was used for in vitro evaluations. Overall, EFE could improve the productive performance of dairy cows and beef cattle, but the response depends upon the proper mixture of Cel and Xyl according to the diet composition. Abbreviations: ADF: acid detergent fiber; ADG: average daily gain; A:P: acetate:propionate ratio; BW: initial body weight; Cel:Xyl: cellulases:xylanases; DM: dry matter; DMD: in vivo dry matter digestibility; DMI: dry matter intake; EA: enzyme activities; EFE: exogenous fibrolytic enzymes; F: type of forage; FC: feed conversion; F:C: forage-to-concentrate ratio; ISDMD: in situ dry matter disappearance; ISNDFD: in situ neutral detergent fiber disappearance; IVADFD: in vitro acid detergent degradability; IVDMD: in vitro dry matter degradability; IVNDFD: in vitro neutral detergent fiber degradability; NDF: neutral detergent fiber; VFA: in vitro volatile fat acid