The Spatiotemporal Variation of Tree Cover in the Loess Plateau of China after the ‘Grain for Green’ Project

Analyzing spatiotemporal variation of tree cover could enhance understanding of the environment and promote a sustainable resource use of ecosystems. This study investigated the variation in tree cover in the Loess Plateau after an ecological restoration effort called the ‘Grain for Green Project’ (GGP). The results show that the proportion of tree covered area in the Loess Plateau changed from 73% to 88%, with the cumulative tree cover fluctuating from approximately 7% to 11%, and the average annual tree cover increased from 10% in 2000 to 12% in 2014. Based on tree cover values over the course of 15 years, the study area was classified into five regions, which provide much more information for spatial assessment of tree cover change in the Loess Plateau spatially. The increase in tree cover value was mainly in the core part of Loess Plateau, the mountains, and the edge of the mountain areas; whereas the values were stable in 36.21% of the area, and a decrease was noted in 5.63% of the area, primarily located in the low plain areas. Approximately 26.36% of the Loess Plateau will show a sustained increase in tree cover in the future. The results of this study will facilitate us to understand the current conditions and development of the GGP’s effects, and offer a valuable reference for future detection of tree cover change through geographic information system (GIS) and remote sensing (RS) tools

p16 and p53 can Serve as Prognostic Markers for Head and Neck Squamous Cell Carcinoma

Objective: The present study aimed to explore the expression and clinical significance of human papilloma virus-related pathogenic factors (p16, cyclin D1, p53) in patients with head and neck squamous cell carcinoma (HNSCC) and construct a predictive model. Methods: The Cancer Genome Atlas was used to obtain clinical data for 112 patients with HNSCC. Expression of p16, p53, and cyclin D1 was quantified. We used the survival package of the R program to set the cut-off value. Values above the cut-off were considered positive, while values below the cut-off were negative. Kaplan–Meier analysis and univariate and multivariate Cox regression analyses were performed to investigate prognostic clinicopathological indicators and the expression of p16, p53, and cyclin D1. A predictive model was constructed based on the results of multifactor Cox regression analysis, and the accuracy of the predictive model was verified through final calibration analysis. Follow-up of patients with HNSCC at the Affiliated Hospital of Binzhou Medical University was conducted from 2015 to 2017, and reliability of the predictive model was validated based on follow-up data and molecular expression levels. Results: According to the results, expression of p16 and p53 was significantly associated with prognosis (P < .05). The predictive model constructed based on the expression levels of p16 and p53 was useful for evaluating the prognosis of patients with HNSCC. The predictive model was validated using follow-up data obtained from the hospital, and the trend of the follow-up results was consistent with the predictive model. Conclusion: p16 and p53 can be used as key indicators to predict the prognosis of HNSCC patients and as critical immunohistochemical indicators in clinical practice. The survival model constructed based on p16 and p53 expression levels reliably predicts patient prognosis

Effects of national ecological restoration projects on carbon sequestration in China from 2001 to 2010

The long-term stressful utilization of forests and grasslands has led to ecosystem degradation and C loss. Since the late 1970s China has launched six key national ecological restoration projects to protect its environment and restore degraded ecosystems. Here, we conducted a large-scale field investigation and a literature survey of biomass and soil C in China's forest, shrubland, and grassland ecosystems across the regions where the six projects were implemented (similar to 16% of the country's land area). We investigated the changes in the C stocks of these ecosystems to evaluate the contributions of the projects to the country's C sink between 2001 and 2010. Over this decade, we estimated that the total annual C sink in the project region was 132 Tg C per y (1 Tg = 10(12) g), over half of which (74 Tg C per y, 56%) was attributed to the implementation of the projects. Our results demonstrate that these restoration projects have substantially contributed to CO2 mitigation in China

The draft genome of watermelon (Citrullus lanatus) and resequencing of 20 diverse accessions

Watermelon, Citrullus lanatus, is an important cucurbit crop grown throughout the world. Here we report a high-quality draft genome sequence of the east Asia watermelon cultivar 97103 (2n = 2x = 22) containing 23,440 predicted protein-coding genes. Comparative genomics analysis provided an evolutionary scenario for the origin of the 11 watermelon chromosomes derived from a 7-chromosome paleohexaploid eudicot ancestor. Resequencing of 20 watermelon accessions representing three different C. lanatus subspecies produced numerous haplotypes and identified the extent of genetic diversity and population structure of watermelon germplasm. Genomic regions that were preferentially selected during domestication were identified. Many disease-resistance genes were also found to be lost during domestication. In addition, integrative genomic and transcriptomic analyses yielded important insights into aspects of phloem-based vascular signaling in common between watermelon and cucumber and identified genes crucial to valuable fruit-quality traits, including sugar accumulation and citrulline metabolism