Cancer Cell Membrane Nanodiscs for Antitumor Vaccination.

Cell membrane-based nanovaccines have demonstrated attractive features due to their inherently multiantigenic nature and ability to be formulated with adjuvants. Here, we report on cellular nanodiscs fabricated from cancer cell membranes and incorporated with a lipid-based adjuvant for antitumor vaccination. The cellular nanodiscs, with their small size and discoidal shape, are readily taken up by antigen-presenting cells and drain efficiently to the lymph nodes. Due to its highly immunostimulatory properties, the nanodisc vaccine effectively stimulates the immune system and promotes tumor-specific immunity. Using a murine colorectal cancer model, strong control of tumor growth is achieved in both prophylactic and therapeutic settings, particularly in combination with checkpoint blockades. Considerable therapeutic efficacy is also observed in treating a weakly immunogenic metastatic melanoma model. This work presents a new paradigm for the design of multiantigenic nanovaccines that can effectively activate antitumor immune responses and may be applicable to a wide range of cancers

Correction: Humoral immune response to inactivated COVID-19 vaccination at the 3rd month among people living with HIV

Correction to "Humoral immune response to inactivated COVID-19 vaccination at the 3rd month among people living with HIV

Perencanaan Sistem Pengelolaan Sampah Terpadu di Kawasan Pasar Flamboyan Kota Pontianak

Sampah merupakan konsekuensi kehidupan yang sering menimbulkan masalah, dan jumlahnya akan semakin meningkat seiring dengan peningkatan jumlah penduduk dan beragam aktivitasnya.Pasar Flamboyan adalah pasar terbesar di Kalimantan Barat.Pasar ini memiliki ± 1700 pedagang dan dibuka setiap hari.Disana terdapat ruko dengan jumlah 53 unit, kios sebanyak 203 unit dan los sebanyak 1498 unit.Saat ini Pasar Flamboyan belum mempunyai sistem pengelolaan sampah terpadu. Hal tersebut akan mengakibatkan banyaknya jumlah sampah yang dihasilkan dari setiap kegiatan. Sampah yang dihasilkan dari berbagai macam penjualan akan menghasilkan sampah yang beragam pula. Penanganan sampah setiap harinya di Pasar Flamboyan untuk saat ini masih menggunakan cara lama yaitu sampah dikumpulkan ke suatu tempat pembuangan sampah sementara lalu pada sore harinya sampah diangkut oleh pihak dari dinas kebersihan untuk dibawa ke TPA. Tujuan dari perencanaan ini yaitu untuk mengetahui total timbulan sampah dan komposisi sampah yang dihasilkan di kawasan Pasar Flamboyan serta untuk merencanakan sistem pengelolaan sampah terpadu di Kawasan Pasar Flamboyan. Perencanaan pengelolaan sampah di Kawasan meliputi perencanaan dari seluruh aspek operasional pengelolaan sampah yaitu perencanaan pewadahan sampah, pengumpulan dan pengangkutan sampah serta pengolahan sampah di Kawasan Pasar Flamboyan. Pengambilan data primer dilakukan dengan cara sampling timbulan dan komposisi sampah. Sampling dilakukan dengan menggunakan metode SNI 19-3694-1994 yaitu pengukuran sampah dengan menggunakan sampling box selama delapan hari berturut-turut yang kemudian akan menghasilkan data volume, berat jenis dan komposisi sampah. Digunakan juga kuisioner untuk mengumpulkan data dari pedagang yang berupa daftar pertanyaan yang disampaikan kepada responden untuk dijawab secara tertulis.Sampel sampah yang diambil masing-masing 3 sampel untuk setiap jenis los, kios dan ruko.Pengambilan sampel dilakukan setiap hari pada pukul 10.00 WIB selama delapan hari berturut-turut ke setiap sumber sampah yang telah ditentukan. Dari hasil perhitungan didapatkan bahwa total timbulan sampah di Kawasan Pasar Flamboyan adalah sebanyak 9,0370 m3/hari sampah organik dan sebanyak 1,0503 m3/hari sampah anorganik. Jumlah pewadahan di tiap sumber sampah ditentukan dari perhitungan rata-rata volume sampah perhari dibagi dengan ukuran tong sampah yang akan digunakan pada sumber tersebut. Jumlah pewadahan yang dibutuhkan untuk tiap sumber sampah Pasar Flamboyan untuk kios dan ruko yaitu sebanyak 79 buah tong sampah ukuran 10 liter dan 95 buah tong sampah ukuran 20 liter. Jumlah alat angkut sampah yang dibutuhkan untuk Pasar Flamboyan yaitu sebanyak 3 buah gerobak.TPST di Kawasan Pasar Flamboyan direncanakan akan berlokasi di bagian belakang Pasar Flamboyan. Jumlah lahan yang dibutuhkan untuk pembangunan TPST Pasar Flamboyan adalah seluas 207,27 m2. Rencana anggaran biaya untuk biaya investasi yaitu sebesar Rp 960.642.206,00. Laba yang diperoleh dari hasil pengolahan sampah yaitu Rp 39.600.000,00/tahun. Biaya hasil retribusi kebersihan dari pedagang yaitu Rp 505.152.000,00/tahun. Dana yang akan dikeluarkan Pasar Flamboyan untuk operasional dan pemeliharaan yaitu Rp 254.760.000,00/tahun

CDCA2 Inhibits Apoptosis and Promotes Cell Proliferation in Prostate Cancer and Is Directly Regulated by HIF-1α Pathway.

Prostate cancer (PCa) is a major serious malignant tumor and is commonly diagnosed in older men. Identification of novel cancer-related genes in PCa is important for understanding its tumorigenesis mechanism and developing new therapies against PCa. Here, we used RNA sequencing to identify the specific genes, which are upregulated in PCa cell lines and tissues. The cell division cycle associated protein (CDCA) family, which plays a critical role in cell division and proliferation, is upregulated in the PCa cell lines of our RNA-Sequencing data. Moreover, we found that CDCA2 is overexpressed, and its protein level positively correlates with its histological grade, clinical stage, and Gleason Score. CDCA2 was further found to be upregulated and correlated with poor prognosis and patient survival in multiple cancer types in The Cancer Genome Atlas (TCGA) dataset. The functional study suggests that inhibition of CDCA2 will lead to apoptosis and lower proliferation in vitro. Silencing of CDCA2 also repressed tumor growth in vivo. Loss of CDCA2 affects several oncogenic pathways, including MAPK signaling. In addition, we further demonstrated that CDCA2 was induced in hypoxia and directly regulated by the HIF-1α/Smad3 complex. Thus, our data indicate that CDCA2 could act as an oncogene and is regulated by hypoxia and the HIF-1αpathway. CDCA2 may be a useful prognostic biomarker and potential therapeutic target for PCa

Petrogenesis of middle Okinawa Trough volcanic rocks: Constraints from lead isotopes in olivine-hosted melt inclusions

Olivine-hosted melt inclusions provide crucial information about their parental magma composition and evolution and represent ideal targets for determining the nature of the poorly understood and still-debated magma sources in the Okinawa Trough, a back-arc basin in the western Pacific. We present the first analyses of the lead (Pb) isotope compositions of olivine-hosted melt inclusions to evaluate the mantle properties and petrogenesis of middle Okinawa Trough volcanic rocks. The melt inclusions have more variable major and trace elements and Pb isotope compositions than the host whole-rock samples. We report the discovery of both high-207Pb/206Pb (> 0.865) and low-207Pb/206Pb (< 0.865) isotope compositions in melt inclusions in individual volcanic rocks, even within a single host olivine, indicating a compositionally heterogeneous magma source. The trace element and Pb isotope characteristics of the melt inclusions show that the magma source is affected by enriched components. We modeled the injection of enriched components into a magma source to explain the generation of the magma heterogeneity. The results indicate that the mixing of Pacific Ocean-type mantle (MORB), an EMI-like component from recycled lower continental crust and EMII-like material from subducted sediments can explain the low-207Pb/206Pb isotope values observed in the melt inclusions. The discovery of small proportions of melt inclusions with high 207Pb/206Pb ratios, high K2O, P2O5, Rb and U contents and low Pb and Cu contents in the studied andesites suggests that the andesitic magma may have been formed by the mixing of materials with different elemental and isotopic compositions. Our study results suggest that pervasive magma mixing may have occurred in the magma source prior to eruption in the Okinawa Trough.This work was supported by the National Natural Science Foundation of China (Grant No. 91958213), the National Programme on Global Change and Air-Sea Interaction (Grant No. GASIGEOGE-02), the Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences (Grant No. MGE2019KG07), the International Partnership Program of Chinese Academy of Sciences (Grant No. 133137KYSB20170003), the National Special Fund for the 13th Five Year Plan of COMRA (Grant No. DY135- G2-1-02), the Special Fund for the Taishan Scholar Program of Shandong Province (Grant No. ts201511061), the Qingdao Postdoctoral Applied Research Project, the National Key Basic Research Program of China (Grant No. 2013CB429700) and Shandong Provincial Natural Science Foundation, China (Grant No. ZR2019BD010)

The Precambrian Khondalite Belt in the Daqingshan area, North China Craton: evidence for multiple metamorphic events in the Palaeoproterozoic era

High-grade pelitic metasedimentary rocks (khondalites) are widely distributed in the northwestern part of the North China Craton and were named the ‘Khondalite Belt’. Prior to the application of zircon geochronology, a stratigraphic division of the supracrustal rocks into several groups was established using interpretative field geology. We report here SHRIMP U–Pb zircon ages and Hf-isotope data on metamorphosed sedimentary and magmatic rocks at Daqingshan, a typical area of the Khondalite Belt. The main conclusions are as follows: (1) The early Precambrian supracrustal rocks belong to three sequences: a 2.56–2.51 Ga supracrustal unit (the previous Sanggan ‘group’), a 2.51–2.45 Ga supracrustal unit (a portion of the previous upper Wulashan ‘group’) and a 2.0–1.95 Ga supracrustal unit (including the previous lower Wulashan ‘group’, a portion of original upper Wulashan ‘group’ and the original Meidaizhao ‘group’) the units thus do not represent a true stratigraphy; (2) Strong tectono-thermal events occurred during the late Neoarchaean to late Palaeoproterozoic, with four episodes recognized: 2.6–2.5, 2.45–2.37, 2.3–2.0 and 1.95–1.85 Ga, with the latest event being consistent with the assembly of the Palaeoproterozoic supercontinent Columbia; (3) During the late Neoarchaean to late Palaeoproterozoic (2.55–2.5, 2.37 and 2.06 Ga) juvenile, mantle-derived material was added to the crust

Light-driven C-H bond activation mediated by 2D transition metal dichalcogenides

C-H bond activation enables the facile synthesis of new chemicals. While C-H activation in short-chain alkanes has been widely investigated, it remains largely unexplored for long-chain organic molecules. Here, we report light-driven C-H activation in complex organic materials mediated by 2D transition metal dichalcogenides (TMDCs) and the resultant solid-state synthesis of luminescent carbon dots in a spatially-resolved fashion. We unravel the efficient H adsorption and a lowered energy barrier of C-C coupling mediated by 2D TMDCs to promote C-H activation. Our results shed light on 2D materials for C-H activation in organic compounds for applications in organic chemistry, environmental remediation, and photonic materials

Light-driven C-H activation mediated by 2D transition metal dichalcogenides

C-H bond activation enables the facile synthesis of new chemicals. While C-H activation in short-chain alkanes has been widely investigated, it remains largely unexplored for long-chain organic molecules. Here, we report light-driven C-H activation in complex organic materials mediated by 2D transition metal dichalcogenides (TMDCs) and the resultant solid-state synthesis of luminescent carbon dots in a spatially-resolved fashion. We unravel the efficient H adsorption and a lowered energy barrier of C-C coupling mediated by 2D TMDCs to promote C-H activation. Our results shed light on 2D materials for C-H activation in organic compounds for applications in organic chemistry, environmental remediation, and photonic materials