Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes

The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmissionrroutes being through the ingestion of contaminated drinking water and food. To date, only limited studies of virulence traits in this genus have been undertaken. The present study used 60 Arcobacter strains isolated from different sources, representing 16 of the 17 species of the genus, to investigate their ability to adhere to and invade the human intestinal cell line Caco-2. In addition, the presence of five putative virulence genes (ciaB, cadF, cj1349, hecA, and irgA) was screened for in these strains by PCR. All Arcobacter species except A. bivalviorum and Arcobacter sp. strain W63 adhered to Caco-2 cells, and most species (10/16) were invasive. The most invasive species were A. skirrowii, A. cryaerophilus, A. butzleri, and A. defluvii. All invasive strains were positive for ciaB (encoding a putative invasion protein). Other putative virulence genes were present in other species, i.e., A. butzleri (cadF, cj1349, irgA, and hecA), A. trophiarum (cj1349), A. ellisii (cj1349), and A. defluvii (irgA). No virulence genes were detected in strains which showed little or no invasion of Caco-2 cells. These results indicate that many Arcobacter species are potential pathogens of humans and animals

Strong normalization for System F by HOAS on top of FOAS

We present a point of view concerning HOAS (Higher-Order Abstract Syntax) and an extensive exercise in HOAS along this point of view. The point of view is that HOAS can be soundly and fruitfully regarded as a definitional extension on top of FOAS (First-Order Abstract Syntax). As such, HOAS is not only an encoding technique, but also a higher-order view of a first-order reality. A rich collection of concepts and proof principles is developed inside the standard mathematical universe to give technical life to this point of view. The exercise consists of a new proof of Strong Normalization for System F. The concepts and results presented here have been formalized in the theorem prover Isabelle/HOL

External Parasites of Swine.

4 p

Carpet Beetles.

1 p

Silverfish.

1 p

Powder Post Beetles.

2 p

Control of Insects in Stored Grain.

2 p

Clothes Moths.

2 p

Scorpions.

2 p

Control of Insects and Allied Pests in the Flower Garden.

3 p