Animal Models to Study the Role of Long-Term Hypergastrinemia in Gastric Carcinogenesis

Patients with chronic hypergastrinemia due to chronic atrophic gastritis or gastrinomas have an increased risk of developing gastric malignancy, and it has been questioned whether also patients with hypergastrinemia caused by long-term use of acid inhibiting drugs are at risk. Gastric carcinogenesis in humans is affected by numerous factors and progresses slowly over years. When using animal models with the possibility of intervention, a complex process can be dissected by studying the role of hypergastrinemia in carcinogenesis within a relatively short period of time. We have reviewed findings from relevant models where gastric changes in animal models of long-term hypergastrinemia have been investigated. In all species where long-term hypergastrinemia has been induced, there is an increased risk of gastric malignancy. There is evidence that hypergastrinemia is a common causative factor in carcinogenesis in the oxyntic mucosa, while other cofactors may vary in the different models

Genetic Rescue of the Highly Inbred Norwegian Lundehund

Augmenting the genetic diversity of small, inbred populations by the introduction of new individuals is often termed “genetic rescue“. An example is the Norwegian Lundehund, a small spitz dog with inbreeding-related health problems that is being crossed with three Nordic breeds, in-cluding the Norwegian Buhund. Conservation breeding decisions for the (typically) small number of outcrossed individuals are vital for managing the rescue process, and we genotyped the Lundehund (n = 12), the Buhund (n = 12), their crosses (F1, n = 7) and first-generation backcrosses to the Lundehund (F2, n = 12) with >170,000 single nucleotide polymorphism loci to compare their levels of genetic diversity. We predicted that genome-wide diversity in F2 dogs would be higher than in the Lundehund but lower than in the F1 and the Buhund, and the heterozygosity values showed the expected patterns. We also found that runs of homozygosity, extended chromosomal regions of homozygous genotypes inherited from a common ancestor, were reduced in F2 individuals compared with Lundehund individuals. Our analyses demonstrate the benefits of outcrossing but indicate that some of the acquired genetic diversity is lost following immediate backcrossing. Additional breeding among F2 crosses could therefore merit from further consideration in genetic rescue management.publishedVersio

Transpancreatic biliary sphincterotomy versus double guidewire in difficult biliary cannulation : a randomized controlled trial

Background Difficult biliary cannulation in endoscopic retrograde cholangiopancreatography (ERCP) increases the risk of post-ERCP pancreatitis (PEP). The purpose of this prospective, randomized, multicenter study was to compare two advanced rescue methods, transpancreatic biliary sphincterotomy (TPBS) and a double-guidewire (DGW) technique, in difficult common bile duct (CBD) cannulation. Methods Patients with native papilla and planned CBD cannulation were recruited at eight Scandinavian hospitals. An experienced endoscopist attempted CBD cannulation with wire-guided cannulation. If the procedure fulfilled the definition of difficult cannulation and a guidewire entered the pancreatic duct, randomization to either TPBS or to DGW was performed. If the randomized method failed, any method available was performed. The primary end point was the frequency of PEP and the secondary end points included successful cannulation with the randomized method. Results In total, 1190 patients were recruited and 203 (17.1%) were randomized according to the study protocol (TPBS 104 and DGW 99). PEP developed in 14/104 patients (13.5%) in the TPBS group and 16/99 patients (16.2%) in the DGW group (P=0.69). No difference existed in PEP severity between the groups. The rate of successful deep biliary cannulation was significantly higher with TPBS (84.6% [88/104]) than with DGW (69.7% [69/99]; P=0.01). Conclusions In difficult biliary cannulation, there was no difference in PEP rate between TPBS and DGW techniques. TPBS is a good alternative in cases of difficult cannulation when the guidewire is in the pancreatic duct.Peer reviewe

Association of urogenital symptoms with history of water contact in young women in areas endemic for <i>S. haematobium</i>:a cross-sectional study in rural South Africa

Female genital schistosomiasis is a neglected tropical disease caused by Schistosoma haematobium. Infected females may suffer from symptoms mimicking sexually transmitted infections. We explored if self-reported history of unsafe water contact could be used as a simple predictor of genital schistosomiasis. In a cross-sectional study in rural South Africa, 883 sexually active women aged 16–22 years were included. Questions were asked about urogenital symptoms and water contact history. Urine samples were tested for S. haematobium ova. A score based on self-reported water contact was calculated and the association with symptoms was explored while adjusting for other genital infections using multivariable logistic regression analyses. S. haematobium ova were detected in the urine of 30.5% of subjects. Having ova in the urine was associated with the water contact score (p &lt; 0.001). Symptoms that were associated with water contact included burning sensation in the genitals (p = 0.005), spot bleeding (p = 0.012), abnormal discharge smell (p = 0.018), bloody discharge (p = 0.020), genital ulcer (p = 0.038), red urine (p &lt; 0.001), stress incontinence (p = 0.001) and lower abdominal pain (p = 0.028). In S. haematobium endemic areas, self-reported water contact was strongly associated with urogenital symptoms. In low-resource settings, a simple history including risk of water contact behaviour can serve as an indicator of urogenital schistosomiasis

Production of charged pions, kaons and protons at large transverse momenta in pp and Pb-Pb collisions at sNN\sqrt{s_{NN}} = 2.76 TeV

Transverse momentum spectra of $\pi^{\pm}, K^{\pm}$ and $p(\bar{p})$ up to $p_T$ = 20 GeV/c at mid-rapidity, |y| $\le$ 0.8, in pp and Pb-Pb collisions at $\sqrt{s_{NN}}$ = 2.76 TeV have been measured using the ALICE detector at the LHC. At intermediate $p_T$ (2-8 GeV/c) an enhancement of the proton-to-proton ratio, (p + \bar{p})/(\pi^+ + \pi^-\(), with respect to pp collisions is observed and the ratio reaches 0.80 in central Pb-Pb collisions. The measurement of the nuclear modification factors for \(\pi^{\pm}, K^{\pm} and $p(\bar{p})$ indicates that within the systematic and statistical uncertainties they are the same at high $p_T$ (> 10 GeV/c), suggesting that the chemical composition of leading particles from jets in the medium is similar to that of vacuum jets.publishedVersio

Multiplicity dependence of the average transverse momentum in pp, p-Pb, and Pb-Pb collisions at the LHC

The average transverse momentum versus the charged-particle multiplicity $N_{ch}$ was measured in p-Pb collisions at a collision energy per nucleon-nucleon pair $\sqrt{s_{NN}}$ = 5.02 TeV and in pp collisions at collision energies of $\sqrt{s}$ = 0.9, 2.76, and 7 Tev in the kinematic range 0.15 with $N_{ch}$ is observed, which is much stronger than that measured in Pb-Pb collisions. For pp collisions, this could be attributed, within a model of hadronizing strings, to multiple-parton interactions and to a final-state color reconnection mechanism. The data in p-Pb and Pb-Pb collisions cannot be described by an incoherent superposition of nucleon-nucleon collisions and pose a challenge to most of the event generators.publishedVersio

Charmonium and e+e−e^+e^- pair photoproduction at mid-rapidity in ultra-peripheral Pb-Pb collisions at sNN\sqrt{s_{NN}} = 2.76 TeV

The ALICE Collaboration at the LHC has measured the $J/\psi$ and $\psi$' photoproduction at mid-rapidity in ultra-peripheral Pb-Pb collisions at $\sqrt{s_{NN}}$ = 2.76 TeV. The charmonium is identified via its leptonic decay for events where the hadronic activity is required to be minimal. The analysis is based on an event sample corresponding to an integrated luminosity of about 23 ${\mu}b^{-1}$, The cross section for coherent and incoherent $J/\psi$ production in the rapidity interval -0.9 < y < 0.9, are $d\sigma_{J/\psi}^{coh}/dy = 2.38^{+0.34}_{-0.24}(sta+sys)$ mb and $d\sigma_{J/\psi}^{inc}/dy = 0.98^{+0.19}_{-0.17}(sta+sys)$ mb, respectively. The results are compared to theoretical models for $J/\psi$ production and the coherent cross section is found to be in good agreement with those models which include nuclear gluon shadowing consistent with EPS09 parametrization. In addition the cross section for the process $\gamma\gamma \to e^+e^-$ has been measured and found to be in agreement with the STARLIGHT Monte Carlo predictions.publishedVersio

Mid-rapidity anti-baryon to baryon ratios in pp collisions at s\sqrt{s} = 0.9, 2.76 and 7 TeV measured by ALICE

The ratios of yields of anti-baryons to baryons probes the mechanisms of baryon-number transport. Results for anti-proton/proton, anti-$\Lambda/\Lambda$, anti-$\Xi^{+}/\Xi^{-}$ and anti-$\Omega^{+}/\Omega^{-}$ in pp collisions at $\sqrt{s}$ = 0.9, 2.76 and 7 TeV, measured with the ALICE detector at the LHC, are reported. Within the experimental uncertainties and ranges covered by our measurement, these ratios are independent of rapidity, transverse momentum and multiplicity for all measured energies. The results are compared to expectations from event generators, such as PYTHIA and HIJING/B, that are used to model the particle production in pp collisions. The energy dependence of anti-proton/proton, anti-$\Lambda/\Lambda$, anti-$\Xi^{+}/\Xi^{-}$ and anti-$\Omega^{+}/\Omega^{-}$, reaching values compatible with unity for $\sqrt{s}$ = 7 TeV, complement the earlier anti-proton/proton measurement of ALICE. These dependencies can be described by exchanges with the Regge-trajectory intercept of $\alpha_J$ ≈ 0.5, which are suppressed with increasing rapidity interval $\Delta y$, Any significant contribution of an exchange not suppressed at large $\Delta y$ (reached at LHC energies) is disfavoured.publishedVersio

Measurement of the Cross Section for Electromagnetic Dissociation with Neutron Emission in Pb-Pb Collisions at √sNN = 2.76 TeV

The first measurement of neutron emission in electromagnetic dissociation of 208Pb nuclei at the LHC is presented. The measurement is performed using the neutron Zero Degree Calorimeters of the ALICE experiment, which detect neutral particles close to beam rapidity. The measured cross sections of single and mutual electromagnetic dissociation of Pb nuclei at √sNN = 2.76 TeV with neutron emission are σ_single EMD = 187.2±0.2 (stat.) +13.8−12.0 (syst.) b and σ_mutual EMD = 6.2 ± 0.1 (stat.) ±0.4 (syst.) b respectively. The experimental results are compared to the predictions from a relativistic electromagnetic dissociation model.publishedVersio

Energy dependence of the transverse momentum distributions of charged particles in pp collisions measured by ALICE

Differential cross sections of charged particles in inelastic pp collisions as a function of $p_T$ have been measured at $\sqrt{s}$ = 0.9, 2.76 and 7 TeV at the LHC. The $p_T$ spectra are compared to NLO-pQCD calculations. Though the differential cross section for an individual $\sqrt{s}$ cannot be described by NLO-pQCD, the relative increase of cross section with $\sqrt{s}$ is in agreement with NLO-pQCD. Based on these measurements and observations, procedures are discussed to construct pp reference spectra at $\sqrt{s}$ = 2.76 and 5.02 TeV up to $p_T$ = 50 GeV/c as required for the calculation of the nuclear modification factor in nucleus-nucleus and proton-nucleus collisions.publishedVersio