24 research outputs found
Wireless Sensor Network (WSN) Platform for Railway Condition Monitoring
In recent years, the sensors are cheaper and are widely available, and also many sensing technologies are rapidly emerging. Sensors are available in a compact size and hence can be installed at inaccessible situations with less effort. They are deployed on a large scale in various industries because of their low cost and discrete varieties. Due to their broad range, there is a rapid expansion in the condition monitoring of the rail systems, for instance, bridges, rail tracks, signaling, etc. Condition monitoring reduces the risks and time involved during human inspections by automated condition monitoring, and it also reduces the maintenance by identifying faults before they get worse and improves safety and reliability. With the rapid improvements in wireless communications, wireless sensor networks (WSNs) are used to monitor these infrastructures effectively using MEMS sensors. WSN condition monitoring reduces the risks, increases the reliability and provide secure communication using the IEEE 802.15.4 protocol. The paper summarizes a background and a literature survey on the WSNs in remote condition monitoring. This paper presents the application of remote condition monitoring by using the ADXL 335 MEMS accelerometer sensor. The sensor records the acceleration of the train and the signal is pre-processed before sending it to the WSN. The output is received at the base station wirelessly via WSN
Engineered FGF1 and FGF2 compositions and methods of use thereof
Engineered FGF1 and FGF2 polypeptides, polynucleotides encoding these polypeptides and DNA constructs, vectors and compositions including these engineered polypeptides are provided herein. The engineered FGF1 and FGF2 polypeptides are more stable than their wild-type counterparts and may be more effective at treating a variety of conditions that FGF1 and FGF2 are useful for treating such as wound healing
Serine protease identification (in vitro) and molecular structure predictions (in silico) from a phytopathogenic fungus, Alternaria solani
Citation: Chandrasekaran, M., Chandrasekar, R., Sa, T., & Sathiyabama, M. (2014). Serine
protease identification (in vitro) and molecular structure predictions (in silico) from a
phytopathogenic fungus, Alternaria solani. Retrieved from http://krex.ksu.eduSerine proteases generally share a relatively high degree of sequence identity and play a major role in the diversity of biological processes. Here we focus on three-dimensional molecular architecture of serine proteases from Alernaria solani. The difference in flexibility of active binding pockets and electrostatic surface potential distribution of serine proteases in comparison with other fungal species is reported in this study. In this study we have purified a serine protease from the early blight pathogen, Alernaria solani. MALDI-TOF-MS/MS analysis revealed that protease produced by A. solani belongs to alkaline serine proteases. AsP is made up of 403 amino acid residues with molecular weight of 42.1kDa (Isoelectric point (pI)-6.51) and molecular formula C[subscript 1859]H[subscript 2930]N[subscript 516]O[subscript 595]S[subscript 4]. The follow-up research on the molecular structure prediction is used for assessing the quality of A. solani Protease (AsP). The AsP protein structure model was built based on its comparative homology with serine protease using the program, MODELER. AsP had 16 β-sheets and 10 α-helices, with Ser[superscript 350] (G347-G357), Asp[superscript 158] (D158-H169) and His[superscript 193] (H193-G203) in separate turn/coil structures. Biological metal binding region situated near the 6th-helix and His[superscript 193] residue is responsible for metal binding site. In addition, the calcium ion is coordinated by the carboxyl groups of Lys[superscript 84], Ile[superscript 85], Lys[superscript 86], Asp[superscript 87], Phe[superscript 88], Ala[superscript 89], Ala[superscript 90] (K84-A90) for first calcium (Ca[superscript 2+]) binding site and carbonyl oxygen atom of Lys[superscript 244], Gly[superscript 245], Arg[superscript 246], Thr[superscript 247], Lys[superscript 248], Lys[superscript 249], and Ala[superscript 250] (K244–A250), for second Ca[superscript 2+] binding site. Moreover, Ramachandran plot analysis of protein residues falling into most favored secondary structures were determined (83.3%). The predicted molecular 3D structural model was further verified using PROCHECK, ERRAT and VADAR servers to confirm the geometry and stereo-chemical parameters of the molecular structural design. The functional analysis of AsP 3D molecular structure predictions familiar in the current study may provide a new perspective in the understanding and identification of antifungal protease inhibitor designing
Mahanine exerts in vitro and in vivo antileishmanial activity by modulation of redox homeostasis
Earlier we have established a carbazole alkaloid (mahanine) isolated from an Indian edible medicinal
plant as an anticancer agent with minimal effect on normal cells. Here we report for the first time that
mahanine-treated drug resistant and sensitive virulent Leishmania donovani promastigotes underwent apoptosis through phosphatidylserine externalization, DNA fragmentation and cell cycle arrest. An early induction of reactive oxygen species (ROS) suggests that the mahanine-induced apoptosis was mediated by oxidative stress. Additionally, mahanine-treated Leishmania-infected macrophages exhibited anti-amastigote activity by nitric oxide (NO)/ROS generation along with suppression of uncoupling protein 2 and Th1-biased cytokines response through modulating STAT pathway. Moreover, we have demonstrated the interaction of a few antioxidant enzymes present in parasite with mahanine
through molecular modeling. Reduced genetic and protein level expression of one such enzyme namely ascorbate peroxidase was also observed in mahanine-treated promastigotes. Furthermore, oral administration of mahanine in acute murine model exhibited almost complete reduction of parasite burden, upregulation of NO/iNOS/ROS/IL-12 and T cell proliferation. Taken together, we have established a new function of mahanine as a potent antileishmanial molecule, capable of inducing ROS and exploit antioxidant enzymes in parasite along with modulation of host’s immune response which
could be developed as an inexpensive and nontoxic therapeutics either alone or in combination
<i>IN SILICO</i> AND <i>IN VITRO</i> STUDIES: TRYPAREDOXIN PEROXIDASE INHIBITOR ACTIVITY OF METHOTREXATE FOR ANTILEISHMANIAL ACTIVITY
Identification of 3-Methoxycarpachromene and Masticadienonic Acid as New Target Inhibitors against Trypanothione Reductase from Leishmania Infantum Using Molecular Docking and ADMET Prediction
Optimization of media composition for D-amino acid oxidase production by <i>Trigonopsis variabilis </i><span style="mso-bidi-font-style:italic">using biostatistical analysis </span>
272-278D-amino acid
oxidase (DAAO) is biotechnologically relevant enzyme that is used in various
food and pharmaceutical industries. <span style="letter-spacing:
-.1pt;mso-ansi-language:EN" lang="EN">DAAO from the yeast Trigonopsis
variabilis<span style="letter-spacing:-.1pt;mso-ansi-language:
EN" lang="EN"> is an important agent for use in commercial applications because of its
high activity with cephalosporin C and is reasonable resistant to the oxidants
O2 and H2O2 byproducts of reaction. In this study, response surface
methodology (RSM) in shake flask culture was used to enhance the production of
DAAO from T. variabilis by
optimization of fermentation media composition. The effects of six factors (DL-alanine, glucose, pH, ZnSO4, (NH4)2SO4
and temperature) were evaluated on DAAO production. Results of Placket-Burman
design showed that DL-alanine, pH, glucose and ZnSO4 were
significant factors for DAAO production (P<0.05). The optimum values of
media components as predicted by the central composite design were inducer
(DL-alanine) concentration 3 g/L, pH 7.7, glucose 17 g/L and ZnSO4 34
mg/L. At these optimum values of media composition, maximum production of DAAO
was 153 U/g yeast dry weight. Two-fold increase in DAAO production was achieved
after optimization of the physical parameters by RSM.
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