Water and energy saving bioprocess for bioethanol production from corn grain applying stillage liquid part recirculation

The distillery stillage is a major and arduous byproduct generated during ethanol production in distilleries. The liquid part of this stillage was proved that can be recycled in the ethanol production from corn, without disturbing the fermentation process. The corn seeds were fermented employing the conventional non-pressure method for gelatinizing as well as including a novelty: Recirculation system of distillery stillage liquid part instead of process water. The efficiency of fermentation and main chemical parameters of stillage were estimated. The liquid part of stillage was recycled 28 times. At these conditions distillery yeast Saccharomyces cerevisiae efficiently produced ethanol yielding 79.80% of the theoretical, keeping the vitality and quantity on the same level. However, recirculation of the liquid part of stillage caused protein and potassium increase in the wet cake what makes this product more attractive for fodder supplementation. It was proven that the addition of stillage liquid fraction to the mashing process instead of process water and 28 recirculation cycles in ethanol production from corn constitutes the way which could significantly reduce the water and energy consumption, what essentially reduce whole general production costs without ethanol efficiency decreasing.Keywords: Corn, stillage liquid part, recirculation, ethanol yieldAfrican Journal of Biotechnology Vol. 12(40), pp. 5950-595

Sport technique as a determinant of athletes’ personality

Background & Aim: In sports, three groups of sports disciplines can be distinguished depending on the way of performing movement actions: a group with a significant degree of kinematic stabilization in the structure of sports technique; a group with a significant degree of stabilization of the dynamic structure of sports technique; a group with a significant degree of variation in sports technique. This study was intended to determine whether sport technique, depending on the degree of stabilisation and variability of movement actions, is a differentiator of athletes’ personality. Method: 90 Polish athletes (men, N=90) from clubs operating in the Polish region of Lower Silesia were purposefully selected for the study as part of three samples: breakdancers (n=30), swimmers (n=30) and shotokan style karatekas (n=30). All the subjects had been considerably successful at international sport competitions. The age of the subjects ranged between 20 and 29 years. The research method chosen was the NEO-FFI Personality Inventory. The basic statistical methods used were a one-way analysis of variance and post-hoc tests. The level of significance was set as the probability of p<0.05. The statistical analysis was performed using the Statistica 13.1 program. Results: Statistically significant differences were revealed in neuroticism among all of the groups studied, in extraversion – between dancers and karatekas, in openness to experience – between dancers and karatekas and between swimmers and karatekas, and in agreeableness – between dancers and karatekas. No statistically significant differences were revealed with regard to conscientiousness. Conclusions: Sport technique is a determinant of athletes’ personality. In addition, sport technique, depending on the degree of stabilisation and variability, is a differentiator of athletes’ personality. Therefore, athletes practising different sport disciplines are characterised by different personalities

Use of Saccharomyces cerevisiae and Zymomonas mobilis for bioethanol production from sugar beet pulp and raw juice

Biofuels have received great attention as an alternative energy source mainly due to limited oil reserves. Bioethanol can be produced from wide range of raw materials like starch, sucrose and cellulosic based sources. Sugar beet and raw juice, as its intermediate product, constitute very profitable substrates for bioethanol production, considering content of easy available fermentable sugars. In this study, sugar beet pulp and raw juice were fermented with Saccharomyces cerevisiae distillery yeasts and bacterium Zymomonas mobilis. Different medium dilution rate as well as yeasts preparations (Fermiol, Safdistil C-70) were investigated. Fermentation was run for 72 h at 30°C. Quality of obtained raw distillates was evaluated using GC method. S. cerevisiae distillery yeasts turned out to be more favourable microorganism than bacterium Z. mobilis for sucrose material fermentation. The ethanol yield obtained from sugar beet pulp and raw juice was 84 and 95% of theoretical yield, respectively. Fermentation of sugar beet raw juice obtained by pressing without enzymatic treatment yielded higher ethanol efficiency as compared to raw juice pressed with enzyme. Dilution ratio 1:1 for fermentation medium appeared to be profitable for effective fermentation process.Keywords: Sugar beet roots, raw juice, fermentation, bioethanol, Saccharomyces cerevisiae, Zymomonas mobilisAfrican Journal of BiotechnologyVol. 12(18), pp. 2464-247

Histidine-Rich C-Terminal Tail of Mycobacterial GroEL1 and Its Copper Complex─The Impact of Point Mutations

The mycobacterial histidine-rich GroEL1 protein differs significantly compared to the well-known methionine/glycine-rich GroEL chaperonin. It was predicted that mycobacterial GroEL1 can play a significant role in the metal homeostasis of Mycobacteria but not, as its analogue, in protein folding. In this paper, we present the properties of the GroEL1 His-rich C-terminus as a ligand for Cu(II) ions. We studied the stoichiometry, stability, and spectroscopic features of copper complexes of the eight model peptides: L1-Ac-DHDHHHGHAH, L2- Ac-DKPAKAEDHDHHHGHAH, and six mutants of L2 in the pH range of 2-11. We revealed the impact of adjacent residues to the His-rich fragment on the complex stability: the presence of Lys and Asp residues significantly increases the stability of the system. The impact of His mutations was also examined: surprisingly, the exchange of each single His to the Gln residue did not disrupt the ability of the ligand to provide three binding sites for Cu(II) ions. Despite the most possible preference of the Cu(II) ion for the His9-His13 residues (Ac-DKPAKAEDHDHHH-) of the model peptide, especially the His11 residue, the study shows that there is not only one possible binding mode for Cu(II). The significance of this phenomenon is very important for the GroEL1 function -if the single mutation occurs naturally, the protein would be still able to interact with the metal ion

(2E)-2-Hydroxy­imino-N′-[(E)-2-pyridyl­methyl­ene]propanohydrazide

In the title compound, C9H10N4O2, the pyridine ring is twisted by 16.5 (1)° from the mean plane defined by the remaining non-H atoms. An intra­molecular N—H⋯N inter­action is present. In the crystal, inter­molecular O—H⋯N and N—H⋯O hydrogen bonds link mol­ecules into layers parallel to the bc plane. The crystal packing exhibits π–π inter­actions indicated by the short distance of 3.649 (1) Å between the centroids of the pyridine rings of neighbouring mol­ecules

(2RS)-3-Hydr­oxy-2-methyl-2-(2-pyrid­yl)imidazolidine-4-one

The title structure, C9H11N3O2, is a racemate. The chiral centre is situated at the N—C—N C atom of the imidazolidine ring. The inter­planar angle between the mean planes of the pyridine and imidazolidine rings is 89.41 (5)°. The methyl group is in a trans position with respect to the pyridine N atom. In the crystal, the mol­ecules are arranged in zigzag layers parallel to the b axis. The mol­ecules within the layers are inter­connected by strong O—H⋯N and weak N—H⋯O hydrogen bonds; the former take place between OH groups and amine N atoms and the latter between the amine N atom and the carbonyl O atom. In addition, C—H⋯O inter­actions are also present

Potassium 2-(N-hydroxy­carbamo­yl)acetate monohydrate

The crystal structure of the title compound, K+·C3H4NO4 −·H2O, consists of potassium cations, monoanions of 2-carboxy­acetohydroxamic acid [namely 2-(N-hydroxy­carbamo­yl)acetate] and solvent water mol­ecules. The elements of the structure are united in a three-dimensional network by numerous K⋯O coordinate bonds and O—H⋯O and N—H⋯O hydrogen bonds. The coordination sphere of the K+ ions may be described as a distorted double capped octa­hedron. Bond lengths and angles are similar to those in related compounds

Diaquabis­[3-(hydroxy­imino)­butanoato]nickel(II)

In the neutral, mononuclear title complex, [Ni(C4H6NO3)2(H2O)2], the Ni atom lies on a crystallographic inversion centre within a distorted octa­hedral N2O4 environment. Two trans-disposed anions of 3-hydroxy­imino­butanoic acid occupy four equatorial sites, coordinated by the deprotonated carboxyl­ate and protonated oxime groups and forming six-membered chelate rings, while the two axial positions are occupied by the water O atoms. The O atom of the oxime group forms an intra­molecular hydrogen bond with the coordinated carboxyl­ate O atom. The complex mol­ecules are linked into chains along b by hydrogen bonds between the water O atom and the carboxyl­ate O of a neighbouring mol­ecule. The chains are linked by further hydrogen bonds into a layer structure

Dibromidobis(3,5-dimethyl-1H-pyrazole-κN 2)cobalt(II)

In the mononuclear title complex, [CoBr2(C5H8N2)2], the CoII atom is coordinated by two N atoms from two monodentate 3,5-dimethyl­pyrazole ligands and two Br atoms in a highly distorted tetra­hedral geometry. In the crystal, the complex mol­ecules are linked by inter­molecular N—H⋯Br hydrogen bonds into chains along [101]. An intra­molecular N—H⋯Br hydrogen bond is also present