Diversity of phytochemical and antioxidant characteristics of black mulberry (morus nigra l.) fruits from Turkey

Black mulberry (Morus nigra L.) fruits are known due to their delicious, sweet and slightly acid flavor and high anthocyanin content. In the present study, the diversity of phytochemical, sensory, and antioxidant characteristics of the fruits of 20 black mulberry genotypes, from the Artvin region of Turkey, were evaluated. As important phytochemical assessments in fruits, we chromatographically (HPLC/DAD) determined glucose (7.22 to 11.10 g/100 g fresh weight (fw)) and fructose content (6.32 and 9.94 g/100 g fw), as well as predominant organic acid in black mulberry genotypes fruits—malic acid (6.02–11.44 g/100 g fw), followed by citric acid. Titrative determination was used for ascorbic acid, finding contents of 17.41–28.33 mg/100 g fw. There was found a great diversity of sensory (taste, juiciness, and aroma) characteristics, indicating a richness of the fruit germplasm. Antioxidant parameters, such as total phenolic (TP) and anthocyanin (TA) content, were assessed spectrometrically; antioxidant activity (AA) was assessed by DPPH and FRAP assays; individual flavonoids and phenolic acids were determined chromatographically (HPLC/DAD). Antioxidant characteristics of the fruits, defined by TP and TA content, ranged from 1951 to 2733 μg GAE/g fw and 508–712 μg C3GE/g fw, respectively. The most abundant compounds of flavonoids and phenolic acids groups were determined to be rutin (47.10–97.20 mg/100 g fw) and chlorogenic acid (51.3–90.8 mg/100 g fw). AA results, measured by the DPPH method as EC50 value, ranged between 16.10 and 25.45 μg/mL; a FRAP assay revealed values of AA between 9.80 and 13.22 μmol TE/g fw. Significant differences in phytochemical and antioxidant qualities were observed among the analyzed M. nigra genotypes. Regarding the best values of phytochemical and antioxidant characteristics, three genotypes of M. nigra were selected to be recommended for fruit production. The results thus highlight the potential for the exploitation of local black mulberry genotypes through crop selection and breeding program.Tomas Bata University in Zlin, Faculty of Technology [IGA FT 2022/004]; Ataturk University, Faculty of Agriculture, Erzurum; Ataturk University, Faculty of Health Sciences, Erzurum; Constantine the Philosopher University in Nitra, Faculty of Central European Studies, NitraIGA FT 2022/004; Atatürk Üniversites

Sensory Evaluation, Biochemical, Bioactive and Antioxidant Properties in Fruits of Wild Blackthorn (<i>Prunus spinosa</i> L.) Genotypes from Northeastern Türkiye

Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small farmers constituting a real safety valve for the sustainability of the processing plum value chain. Fruits of those plum genotypes differ in their biometric, processing and functional quality attributes. In this study, fruits of eight wild grown blackthorn (Prunus spinosa) genotypes were sampled from the Ispir district of the Erzurum province and subjected to sensory, morphological, biochemical and antioxidant characterization. Aroma, taste and juiciness were used as the criteria for sensory analysis, and a trained panel of ten experts established and evaluated the sensory characteristics of the fruits of the blackthorn. Fruit weight, fruit skin and flesh color as L*, a* and b* values were the main morphological parameters. For biochemical and bioactive analysis, organic acids, SSC (Soluble Solid Content), vitamin C, total anthocyanins, total phenolic content and total antioxidant capacity were determined. Antioxidant capacity was determined by FRAP (ferric reducing antioxidant power) assay. The results indicated significant differences among genotypes for most of the traits. The fruit weight was found between 2.78–3.67 g. The skin L*, a* and b* values were 13.11–16.12, 2.56–3.85 and 2.01–3.44, respectively. The flesh L*, a* and b* values were in the ranges of 17.45–20.37, 4.88–6.73 and 4.12–5.66, respectively. The SSC content ranged from 18.66% to 21.07%. The total phenolic content (TPC), total anthocyanin content (TAC) and ferric reducing antioxidant power (FRAP) were between 372–504 mg GAE/100 g; 53–72 mg cy-3 g eq./100 g and 107–134 mmol Fe (II) eq./g, respectively. The dominant organic acid was malic acid for all genotypes and varied from 1.04 g/100 g to 1.52 g/100 g fresh weight base. The data showed that the analyzed blackthorns, particularly PS-5, PS-3 and PS-2 had bigger fruits indicate their suitability for fresh and dried consumption, PS-1 and PS-3 had higher juiciness, indicating their suitability for processing, and PS-4 and PS-6 had higher human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants

Characterization of Oleaster-Leafed Pear (<i>Pyrus elaeagrifolia</i> Pall. subsp. <i>elaeagrifolia</i>) Fruits in Turkey

Oleaster-leafed pear (Pyrus elaeagrifolia Pall. subsp. elaeagrifolia) fruits are used for food and dietary supplements in Turkey, and seedlings are used as rootstock for pear cultivars. Information on the effect of genotypes on oleaster-leafed pear fruit characteristics is needed in order to optimize production of quality food and dietary supplements. The characteristics of oleaster-leafed pear fruits relative to genetic background were evaluated from 16 wild grown oleaster-leafed pear genotypes at eastern Turkey. Genotype influenced ripening dates, fruit weight, fruit length/width ratio, fruit pedicel length, fruit flesh texture, fruit firmness, the number of seeds per fruit, soluble solid content, titratable acidity, total phenolic content, total flavonoid content and antioxidant activity. Analysis of the data obtained from 16 oleaster-leafed pear genotypes demonstrated a highly significant influence of genotype on fruit characteristics. The genotypes G12, G13 and G9 had the highest fruit weight (19.22, 18.54 and 18.30 g) and G9 the highest total phenolic content (122 mg gallic acid equivalent/100 g fresh fruit). The genotypes G3, G5, G11 and G13 had the slightly sandy fruit flesh texture and those genotypes may be good selections for processing and producing health oleaster-leafed pear products

Nano-vesicular formulation of propolis and cytotoxic effects in a 3D spheroid model of lung cancer

Ulucan, Fulden/0000-0001-5567-0261; Yesil-Celiktas, Ozlem/0000-0003-4509-2212; saglam metiner, pelin/0000-0002-5726-1928WOS: 000526841800001PubMed: 32239766BACKGROUND Propolis exhibits therapeutic properties due to the presence of phenolic acids, esters, and flavonoids. the scope of this study was to develop a nano-vesicular formulation and establish a three-dimensional (3D) spheroid model in which lung cancer is recapitulated. RESULTS Niosome vesicles doped with galangin-rich propolis extract were synthesized by the ether injection method using a cholesterol : surfactant mass ratio of 1 : 3 at 40 degrees C for 1 h. Formulated niosomes were administered to 3D lung cancer spheroid model and the cytotoxicity was compared with that of a two-dimensional (2D) setting. the galangin content was determined as 86 mu g mg(-1) propolis extract by ultra-performance liquid chromatography (UPLC). the particle size of loaded niosome was 151 +/- 2.84 nm with a polydispersity index (PDI) of about 0.232, and an encapsulation efficiency of 70% was achieved. CONCLUSION the decrease in cell viability and the scattering in the 3D spheroids of A549 lung cancer cells treated with propolis-loaded niosomes were notable, indicating a profound cytotoxic effect and suggesting that they can be utilized as an effective nano-vesicle. (c) 2020 Society of Chemical Industr

Morphological and biochemical diversity among wild-grown carob trees (Ceratonia siliqua L.)

Tree, leaf, pod and seed morphology, as well as pod biochemistry of 36 wild-grown carob genotypes sampled from rural areas in Marmaris district located at western Turkey, were investigated. Leaf and pod dimensions, pod and seed weight, seed ratio, pod and seed colour and shape and surface traits were investigated. Soluble solid content (SSC), titratable acidity, vitamin C and protein and dietary fibre contents were also detected. Results showed significant differences for all quantitative traits, although differences are more pronounced for some pod (weight, width, length and thickness) and seed characteristics (weight, dimensions and ratio). Pod and seed colour, shape and surface qualitative threats were found to be quite variable among genotypes. The majority of genotypes had an open tree growth habit. Leaf length and width were found to be between 8.04 cm (M19) and 11.60 cm (M12) and 8.40 cm (M2) and 12.04 cm (M12) among genotypes. Pod weight ranged from 8.3 g (M35) to 29.5 g (M3) in the wild genotypes. The average pod dimensions (width, length and thickness) were between 14.27 and 23.38 mm, 12.54 and 21.67 cm and 4.80 and 8.37 mm, respectively. The SSC ranged from 49.36 to 69.36% in the pods of wild carob genotypes. The results of this study indicate a good genetic resource potential of Turkish wild carob populations for future breeding programmes

Morphological and Biochemical Characterization of Diverse Strawberry Tree (Arbutus unedo L.) Genotypes from Northern Turkey

Wild edible fruits have been used centuries by local people mainly for nutrition purposes and they have attracted the attention of researchers in the last decade throughout the world. Among wild edible fruits, the strawberry tree (Arbutus unedo) is widely distributed mainly in Mediterranean countries. However, the lack of information about strawberry tree genetic resources in Mediterranean countries is a major cause of its low exploitation by breeders. For a practical use and exploitation of the strawberry tree native germplasm, phenological, morphological, and biochemical characterization of the plant is essential. In this study, some important phenological, morphological, and biochemical characteristics of sixteen local strawberry tree genotypes were determined. Significant variations (p &lt; 0.05) were observed in most of the investigated phenological, morphological, and biochemical traits of the genotypes. The genotypes were harvested between 2&ndash;21 November. The fruit weight and the number of fruits per cluster of the genotypes varied from 3.89 to 6.86 g and 3.61 to 7.94, respectively. Vitamin C and total dietary fiber contents of genotypes were found quite variable, ranging from 58 to 93 mg/100 g and 7.04 to 12.36 g/100 g, respectively. The fruits of the strawberry tree include mainly fructose and glucose rather than sucrose. Local strawberry tree genotypes are found to be promising for further valorization through horticultural characteristics for breeding. Some genotypes had significantly higher fruit weight, vitamin C concentrations, total dietary fiber, and total phenolic concentration and may be good selections for producing health products

Investigations of volatile organic compounds in berries of different Actinidia kolomikta (Rupr. & Maxim.) Maxim. accessions

The composition of volatile organic compounds contributes to fruit fl avour and is an important element of fruit quality. Berries of sixteen cultivars and female clones of Actinidia kolomikta were studied by GC-MS with headspace solid phase microextraction method. In total, 89 compounds were separated and identifi ed. These compounds were classifi ed as terpenes, esters, alcohols, aldehydes, anhydrides, diazoles, hydrocarbons, and ketones. Among volatile organic compounds, esters were the most abundant. Signifi cant differences in the diversity of volatile organic compounds were found among A. kolomikta cultivars and clones. Based on these results, we selected potential accessions for the breeding of new cultivars. Summarising the results of this study, the accessions of A. kolomikta were grouped according to the main fl avour compoundsInstrumentinės analizės APCVilniaus universitetasVytauto Didžiojo universiteta

Improvement of postharvest quality and bioactive compounds content of persimmon fruits after hydrocolloid-based edible coating application

Persimmon fruits are often affected by large postharvest losses due to rapid ripening and the early onset of senescence. To reduce such losses in fresh fruits, the application of hydrocolloid-based edible coatings was conducted. Therefore, a plant hydrocolloid-based gum, tragacanth gum (TCG), was applied to persimmon fruits at 0.5%, 1%, and 1.5% TCG concentrations, and stored at 20 ± 2 °C and 80–85% relative humidity for 20 days (analysis at 0, 4th, 8th, 12th, 16th, and 20th day). As a result of TCG application on persimmon fruits, there were greatly suppressed respiration rates, ethylene production, weight loss, decay incidence, and H2O2 and malondialdehyde content. In addition, TCG-coated persimmon fruits had higher concentrations of bioactive compounds including phenols, flavonoids, carotenoids, ascorbic acid, and soluble tannin. Higher enzymatic antioxidant activities and lower softening enzyme activities were also recorded for TCG-coated persimmon fruits. Uncoated persimmon fruits quickly lost fruit quality attributes like color, firmness, taste, and aroma during storage compared to coated ones. Based on our findings, the use of TCG, especially at the concentration of 1% TCG, can be recommended to be applied as the edible coating to maintain the nutritional, biochemical, and commercial quality of persimmon fruits during ambient storage. © 2022 by the authors.Tomas Bata University in Zlin, TBU: IGA/FT/2022/004internal grant of Tomas Bata University in Zlin [IGA/FT/2022/004

Table_5_Comparative genomic analysis and phylogeny of NAC25 gene from cultivated and wild Coffea species.DOCX

Coffee is a high value agricultural commodity grown in about 80 countries. Sustainable coffee cultivation is hampered by multiple biotic and abiotic stress conditions predominantly driven by climate change. The NAC proteins are plants specific transcription factors associated with various physiological functions in plants which include cell division, secondary wall formation, formation of shoot apical meristem, leaf senescence, flowering embryo and seed development. Besides, they are also involved in biotic and abiotic stress regulation. Due to their ubiquitous influence, studies on NAC transcription factors have gained momentum in different crop plant species. In the present study, NAC25 like transcription factor was isolated and characterized from two cultivated coffee species, Coffea arabica and Coffea canephora and five Indian wild coffee species for the first time. The full-length NAC25 gene varied from 2,456 bp in Coffea jenkinsii to 2,493 bp in C. arabica. In all the seven coffee species, sequencing of the NAC25 gene revealed 3 exons and 2 introns. The NAC25 gene is characterized by a highly conserved 377 bp NAM domain (N-terminus) and a highly variable C terminus region. The sequence analysis revealed an average of one SNP per every 40.92 bp in the coding region and 37.7 bp in the intronic region. Further, the non-synonymous SNPs are 8-11 fold higher compared to synonymous SNPs in the non-coding and coding region of the NAC25 gene, respectively. The expression of NAC25 gene was studied in six different tissue types in C. canephora and higher expression levels were observed in leaf and flower tissues. Further, the relative expression of NAC25 in comparison with the GAPDH gene revealed four folds and eight folds increase in expression levels in green fruit and ripen fruit, respectively. The evolutionary relationship revealed the independent evolution of the NAC25 gene in coffee.</p