Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

An efficient access to the synthesis of novel 12-phenylbenzo[6,7]oxepino[3,4-b]quinolin-13(6H)-one derivatives

An efficient access to the tetracyclic-fused quinoline systems, 12-phenylbenzo[6,7]oxepino[3,4-b]quinolin-13(6H)-one derivatives 4a–l, is described, involving the intramolecular Friedel–Crafts acylation reaction of 2-(phenoxymethyl)-4-phenylquinoline-3-carboxylic acid derivatives 3a–l aided by the treatment with PPA (polyphosphoric acid) or Eaton’s reagent. The required starting compound (2) was obtained by Friedländer reaction of 2-aminobenzophenone (1) with 4-chloroethylacetoacetate by using CAN (cerium ammonium nitrate, 10 mol %) as catalyst at room temperature. The substrates 3a–l were prepared through one-pot reaction of ethyl 2-(chloromethyl)-4-phenylquinoline-3-carboxylate (2) and substituted phenols. Our developed strategy, involving a three-step route, offers easy access to tetracyclic-fused quinoline systems in short reaction times, and the products are obtained in moderate to good yields

China’s Spatial Economic Network and Its Influencing Factors

With the deepening of reform and opening-up, China’s economy has been further developed, but there is still a problem of uneven development. It is of great significance to completely construct China’s economic spatial correlation network, to clarify the role and status of each province in the whole network, and to study the influencing factors of the national spatial economic network. In this paper, we employ the network analysis method to analyze China’s economic development in the past 20 years. Based on the modified gravity model, we construct China’s spatial economic network and explore the network structure from three aspects: the whole network structure feature, characteristics of individual provinces in the network, and block model analysis. The results show that (1) China’s spatial economic network has strong internal cohesion, and the hierarchy of the network is becoming less and less obvious. However, the network density is low, and the overall network relationship still needs to be strengthened. (2) The different levels in economic development illustrate the obvious economic unbalance among provinces. (3) The block model analysis results demonstrated that coastal areas are more attractive to other provinces and are playing an important role in driving China’s economy. Finally, we employ Quadratic Assignment Procedure (QAP) regression analysis to analyze the influential factors on spatial economic network. Numerical results show that the geographic proximity and the differences in six factors (industrial structure, level of economic development, degree of opening to the outside world, medical level, size of labor market, and infrastructure) have significant impact on the spatial economic network. Moreover, the influence of these factors on the economic relation among provinces has been gradually strengthened in recent years