Influência dos cones de guta-percha na obturação de canais laterais artificiais

The aim of this study was to investigate, in vitro, the percentage of filling of simulated lateral canals in teeth obturated with TP medium and standardized gutta-percha points. Twenty human mandibular canines were prepared with LA Axxess (SybronEndo) and K³ Endo rotary system (SybronEndo) up to a #50 file, according to the Free Tip Preparation Technique. During instrumentation, the root canals were alternately irrigated with 1% sodium hypochlorite and 17% EDTA. Six artificial lateral canals were prepared at the apical third of each tooth. Then, the teeth were assigned to two groups (n=10): Group 1 - filled with TP medium master gutta-percha points (Dentsply, Mailleffer); Group 2 - filled with standardized master gutta-percha points (Dentsply, Mailleffer). Root canal filling was complemented with AH Plus sealer (Dentsply, Mailleffer) and accessory gutta-percha points (Dentsply, Mailleffer), according to the classic technique. The teeth were radiographed and the images obtained were digitized. Linear measurements of the percentage of filling of the artificial lateral canals in each group were accomplished on the Image Tool 2.02 software. Statistical analysis of the data using Mann-Whitney U non-parametric test evidenced significant difference (pO objetivo deste estudo foi avaliar in vitro o percentual de preenchimento de canais laterais artificiais em dentes obturados com cones de guta-percha TP medium e estandardizados. Vinte caninos inferiores humanos foram preparados com os instrumentos LA Axxess (SybronEndo) e o sistema rotatório K³ Endo (SybronEndo) até a lima #50, de acordo com a técnica Free Tip Preparation. Durante a instrumentação, os canais radiculares foram alternadamente irrigados com solução de hipoclorito de sódio a 1% e EDTA a 17%. Seis canais laterais artificiais foram confeccionados no terço apical de cada dente. Os dentes foram então divididos em dois grupos (n=10): Grupo 1 - obturados com cones de guta-percha principais TP médium (Dentsply, Mailleffer), Grupo 2 - obturados com cones de guta-percha principais estandardizados (Dentsply, Mailleffer). A obturação do canal radicular foi complementada com o cimento obturador AH Plus (Dentsply, Mailleffer) e cones de guta-percha acessórios (Dentsply, Mailleffer), de acordo com a técnica clássica. Os dentes foram radiografados e as imagens digitalizadas. Realizou-se a mensuração do percentual de obturação dos canais laterais em cada grupo por meio do software Image Tool 2.02. A análise estatística dos dados por meio do teste não paramétrico Mann-Whitney U evidenciou diferença significante (

Study on the effects of some irrigating solutions on dentine microhardness and smear layer removal

Este trabalho estudou os efeitos de algumas soluções irrigadoras sobre a microdureza dentinária e capacidade de remoção da smear layer. As soluções testadas foram: soro fisiológico, hipoclorito de sódio a 2,5%, EDTA a 15% em pH 7,3, EDTA a 15% em pH 9,0, ácido etileno diamino di-succínico (EDDS), Smear Clear® e BioPure MTAD®. Para o estudo de microdureza, 70 fragmentos de dentina medindo 5x5 mm foram obtidos do terço médio de hemissecções radiculares de incisivos bovinos, sendo separados aleatoriamente em sete grupos. A microdureza Knoop inicial da dentina foi aferida por meio de microdurômetro acionado com uma carga de 25 g por 15 s. As amostras eram então tratadas pela imersão nas soluções experimentais por um minuto, após o qual tinham a sua microdureza mensurada. Após mais quatro minutos de imersão nas soluções testadas, os valores de microdureza eram novamente registrados. Os valores de microdureza iniciais e após cada imersão nas soluções testadas foram então submetidos à análise estatística (two-way ANOVA). Para a avaliação da capacidade de remoção da smear layer, 35 raízes de incisivos bovinos foram separadas aleatoriamente em sete grupos e instrumentadas pela técnica step-back. Cada grupo recebeu um tipo de solução irrigadora experimental, ficando estas em contato com as paredes dentinárias por 5 minutos. Os espécimes foram cisalhados e observados ao microscópio eletrônico de varredura com um aumento de 350 vezes para avaliação da remoção da smear layer. Fotomicrografias do terço médio do canal radicular foram obtidas e, por meio de uma grade de integração, foi contabilizado o percentual de áreas contendo smear layer. Os resultados da análise da microdureza dentinária indicaram que, com exceção do soro fisiológico, todas as soluções testadas causaram uma redução significativa desta propriedade. Hipoclorito de sódio a 2,5%, EDDS e Smear Clear® apresentaram resultados semelhantes, causando diminuição da microdureza menos acentuada do que o BioPure MTAD® ou as soluções de EDTA em pH 7,3 ou 9,0. Para a análise de remoção da smear layer, as soluções de EDTA em pH 7,3 ou 9,0, o Smear Clear® e o BioPure MTAD® mostraram resultados semelhantes. O EDDS mostrou semelhanças estatísticas tanto com o hipoclorito de sódio como com as outras soluções quelantes testadas, ocupando uma posição intermediária na capacidade de remoção da smear layer. Pode-se concluir que o EDTA em pHs 7,3 ou 9,0 são estatisticamente semelhantes, bem como o BioPure MTAD®. Enquanto o Smear Clear® é capaz de remover a smear layer sem diminuir a microdureza dentinária, o EDDS reduz moderadamente a microdureza dentinária, porém é deficiente na limpeza das paredes do canal radicular.This study evaluated the effects of some irrigating solutions on dentine microhardness and smear layer removal. The tested solutions were: saline, 2.5% sodium hypochlorite, 15% EDTA in pH 7.3, 15% EDTA in pH 9.0, ethylene diamine dissuccinic acid (EDDS); Smear Clear® and BioPure MTAD®. For the microhardness study, 70 5x5 mm dentine stubs obtained from the middle third of root hemisections were randomly divided in seven groups. The initial Knoop Hardness Values of the samples were assessed by a microhardness tester with a 25 g load, for 15 s. The samples were then immersed on tested solutions for one minute, after which the KHN values were again measured. One more evaluation of microhardness was performed after four more minutes of treatment with the tested solutions. Results were then submitted to statistical analysis (two-way ANOVA). For the smear layer removal study, 35 bovine incisor roots were randomly assigned to seven groups, according to the irrigating solution, and instrumented following the step-back technique. The irrigating solution was left for 5 minutes in the canals after instrumentation, and then washed with distilled water. Samples were then split and observed under the SEM with 350x magnification. Photomicrographs were obtained from the middle third of the root canal and evaluated for the percentage of remaining smear layer. Results of the microhardness tests showed that all solutions yeld to a hardness reduction of dentine, except saline solution. Sodium hypochlorite, EDDS and Smear Clear presented similar results, with mild reduction of microhardness, while MTAD or EDTA solutions presented severe microhardness alterations. Regarding smear layer removal, EDTA solutions, Smear Clear® and BioPure MTAD® presented similar results, with clean root canal walls and no visible débris. EDDS was more effective than saline solution or sodium hypochlorite, but less effective than the other chelating solutions. It can be concluded that EDTA at pH 7.3 or 9.0 are statistically similar, as well as BioPure MTAD®. While Smear Clear® is able to remove smear layer without reducing dentine microhardness, EDDS does reduce it moderately, but without properly cleaning the root canal walls

Evaluation of Low Intensity Laser Therapy In Myofascial Pain Syndrome

Limited studies have demonstrated that low intensity laser therapy (LILT) may have a therapeutic effect on the treatment of myofascial pain syndrome (MPS). Sixty (60) patients with MPS and having one active trigger point in the anterior masseter and anterior temporal muscles were selected and assigned randomly to six groups (n=10): Groups I to III were treated with GaAIAS (780 nm) laser, applied in continuous mode and in a meticulous way, twice a week, for four weeks. Energy was set to 25 J/cm(2), 60 J/cm2 and 105 J/cm2, respectively. Groups IV to VI were treated with placebo applications, simulating the same parameters as the treated groups. Pain scores were assessed just before, then immediately after the fourth application, immediately after the eighth application, at 15 days and one month following treatment. A significant pain reduction was observed over time (p<0.001). The analgesic effect of the LILT was similar to the placebo groups. Using the parameters described in this experiment, LILT was effective in reducing pain experienced by patients with myofascial pain syndrome. Thus, it was not possible to establish a treatment protocol. Analyzing the analgesic effect of LILT suggests it as a possible treatment of MPS and may help to establish a clinical protocol for this therapeutic modality

Effect of different bleaching systems on the ultrastructure of bovine dentin

The aim of this study was to evaluate in vitro the effect of different in-office bleaching systems on the surface morphology of bovine dentin. Thirty tooth fragments measuring 4 x 4mm, containing enamel and dentin, were obtained from the crowns of extracted bovine incisors. Samples were subjected to simulated intracoronal bleaching techniques using conventional (Opalescence Endo (R) and Whiteness Super Endo (R)) and light-activated systems (Opalescence Xtra (R) and Whiteness HP Maxx (R)). Controls were treated with either sodium perborate mixed with 10% hydrogen peroxide or no bleaching agent. The samples were observed under SEM and the recorded images were evaluated for topographic alterations. The ultrastructural alterations of dentin observed in this study varied greatly between groups according to the products used. Higher pH products (Whiteness HP Maxx (R) and Opalescence Xtra (R)) associated with in-office techniques yielded better maintenance of dentin ultrastructure. Apparently, both low pH and hydrogen peroxide oxidation play a role in altering the ultrastructure of dentin during internal dental bleaching. The use of alkaline products with reduced time of application (in-office techniques) may decrease such morphological alterations

Evaluation of the temperature of different refrigerant sprays used as a pulpal test

The temperature of different refrigerant sprays (Endo-Ice, Endo-Frost, Coolermatic and Sprayon Contact and Tuner Cleaner) used as pulpal tests were evaluated in vitro. A thermocouple placed inside the pulp chamber of a maxillary central incisor was used to register the temperature changes when the refrigerant sprays were applied with a cotton swab, for 10 s. Results indicate that Endo-Ice and Endo-Frost presented the lowest temperatures among the refrigerant sprays tested. Temperatures measured inside the pulp chamber, however, were statistically similar in all groups

Influence of gutta-percha points on the filling of simulated lateral canals

The aim of this study was to investigate, in vitro, the percentage of filling of simulated lateral canals in teeth obturated with TP medium and standardized gutta-percha points. Twenty human mandibular canines were prepared with LA Axxess (SybronEndo) and K³ Endo rotary system (SybronEndo) up to a #50 file, according to the Free Tip Preparation Technique. During instrumentation, the root canals were alternately irrigated with 1% sodium hypochlorite and 17% EDTA. Six artificial lateral canals were prepared at the apical third of each tooth. Then, the teeth were assigned to two groups (n=10): Group 1 - filled with TP medium master gutta-percha points (Dentsply, Mailleffer); Group 2 - filled with standardized master gutta-percha points (Dentsply, Mailleffer). Root canal filling was complemented with AH Plus sealer (Dentsply, Mailleffer) and accessory gutta-percha points (Dentsply, Mailleffer), according to the classic technique. The teeth were radiographed and the images obtained were digitized. Linear measurements of the percentage of filling of the artificial lateral canals in each group were accomplished on the Image Tool 2.02 software. Statistical analysis of the data using Mann-Whitney U non-parametric test evidenced significant difference (p<0.01) between the experimental groups. The group obturated with TP medium points yielded higher percentage of filling of the lateral canals. It may be concluded that the use of master gutta-percha points with larger taper resulted in better filling of the simulated lateral canals, as compared to the use of standardized master gutta-percha points

Influence of different kinds of rosins and hydrogenated resins on the setting time of Grossman cements Influência de diferentes tipos de breus e resinas hidrogenadas sobre o tempo de endurecimento dos cimentos do tipo Grossman

In this study, the effect on the setting time by the addition of different kinds of rosin and hydrogenated resin on the Grossman cement powder was evaluated. The experiments were carried out following the American Dental Association’s specification number 57 for root canal sealers. For this analysis, different Grossman cement powders were prepared using different rosins (X, WW and WG) and hydrogenated resins (Staybelite and Staybelite ester 10). The study of the physicochemical properties of the Grossman cements obtained the different kinds of rosins and hydrogenated resins interference on the cement’s setting time. The hydrogenated resin, having a higher pH, increased the setting time of the cement when compared to the X, WW and WG rosins.<br>No presente estudo, analisou-se o efeito da adição de diferentes tipos de breus e resinas hidrogenadas ao pó do cimento de GROSSMAN sobre o tempo de endurecimento. Os experimentos foram realizados de acordo com a Especificação 57 para materiais obturadores de canais radiculares da American Dental Association (ADA). Para análise, foram aviados pós do cimento de GROSSMAN com diferentes tipos de breu (X, WW e WG) e resinas hidrogenadas (Stabylite e Stabylite éster 10). Os estudos das propriedades físico-químicas dos cimentos tipo GROSSMAN obtidos de diferentes tipos de breus e resinas hidrogenadas interferem no tempo de endurecimento do cimento. A resina hidrogenada, obtida do processo de hidrogenação tem o pH mais alto, provocando um aumento do tempo de endurecimento do cimento em relação aos breus tipo X, WW e WG, que têm pH mais ácido