Delivering new sorghum and finger millet innovations for food security and improving livelihoods in Eastern Africa

ILRI works with partners worldwide to help poor people keep their farm animals alive and productive, increase and sustain their livestock and farm productivity, and find profitable markets for their animal products. ILRI’s headquarters are in Nairobi, Kenya; we have a principal campus in Addis Ababa, Ethiopia, and 14 offices in other regions of Africa and Asia. ILRI is part of the Consultative Group on International Agricultural Research (www.cgiar.org), which works to reduce hunger, poverty and environmental degradation in developing countries by generating and sharing relevant agricultural knowledge, technologies and policies

Response of Leucaena leucocephala (Lam.) De Wit (Leucaena) Provenances to Aluminium in Potted Soil Experiment

Aims: To determine the level of acid or aluminium tolerance provenances in Leucaena (Leucaena leucocephala) a favourite agroforestry tree in Kenya. Study Design: The set up was a 2-factor (provenance-aluminium) experiment in a completely randomized design with three (3) replications and data were subjected to multivariate analysis of variance. Place and Duration of Study: Potted acid soil experiments were carried out at the Maseno ICRAF/KEFRI centre and Chepkoilel campus farm, Moi University, between June 2009 and July 2010. Methodology: Potted acid soil experiments were carried out at the Maseno ICRAF/KEFRI centre (pH 4.8) and Chepkoilel campus farm, Moi University (pH 5.0) to assess the effect of varying aluminium concentrations on growth of three local leucaena provenances: K156 (Gede), K136 (Kibwezi) and KIT2724 (Kitale). Aluminium was applied at 0, 100, 200 and 300 M. The number of leaves per seedling, seedling height, root length, root collar diameter and dry weight were recorded at 60 and 120 days after planting. Results: Generally Aluminium at 100 M significantly (p0.05) enhanced growth of the seedlings at both sites. However, aluminium at 200 M reduced seedling growth. Conclusion: The Leucaena provenance K156 could be used in acid soils because it is tolerant. However, more local provenances should be screened for acid tolerance

Combining Drought and Aluminum Toxicity Tolerance To Improve Sorghum Productivity

Moisture stress and aluminium toxicity in sorghum production can be overcome by breeding for tolerance. This study was set up to determine the response of sorghum (Sorghum bicolor L.) genotypes to post- anthesis drought and aluminium toxicity. Sorghum inbred P1 with stay green drought tolerance was crossed with P2, a standard aluminium tolerant cultivar. The parents, the first filial plants (F1) and KM-4 were tested for drought and aluminium toxicity tolerance in the field and solution culture with 0 and \u3bcMol 148 Al3+ respectively. The drought experiment was laid in RCBD with three replicates and split -plot arrangement. Randomly selected plots were denied irrigation from six weeks after emergence. There were significant (P < 0.05) differences between treatments and accessions. Panicle weight of F1 was outstanding and significantly different from the rest both with and without water. F1 out performed P1, P2 and KM-4 by 95.61, 146.37 and 328.81% without water; and by 155.44, 124. 27 and 82.02% with water, respectively. There were significant (P < 0.05) differences in the sorghum accessions with P2, KM-4 and F1 being tolerant. These results indicate that multiple stress tolerance can increase sorghum productivity.Le stress hydrique et la toxicit\ue9 aluminique dans la production de sorgho peuvent \ueatre surmont\ue9s par am\ue9lioration de la tol\ue9rance. Cette \ue9tude \ue9tait faite dans le but de d\ue9terminer la r\ue9ponse des g\ue9notypes du sorgho (Sorghum bicolor L.) \ue0 la s\ue9cheresse post-anth\ue9sis et la toxicit\ue9 aluminique. Le sorgho de lign\ue9e pure P1 dot\ue9 d'une persistence chlorophyllienne caract\ue9risant sa tol\ue9rance \ue0 la s\ue9cheresse, \ue9tait crois\ue9 avec P2, un cultivar de tol\ue9rance standard \ue0 l'aluminium. Les parents, les premi\ue8res plantes filiales (F1) et KM-4 \ue9taient test\ue9s au champs et avec une solution de culture 0 et 148 Al3+ \u3bcMol respectivement pour tol\ue9rance \ue0 la s\ue9cheresse et \ue0 la toxicit\ue9 aluminique. Le dispositif experimental de l'essai sur la s\ue9cheresse \ue9tait en blocs al\ue9atoires compl\ue9tement randomis\ue9s avec trois r\ue9p\ue9titions et un arrangement en split-plot. Les parcelles al\ue9atoirement s\ue9lectionn\ue9es n'\ue9taitent pas arros\ue9es depuis six semaines apr\ue8s \ue9mergence. Des diff\ue9rences significatives (P < 0.05) \ue9taient observ\ue9es entre traitements et accessions. Le poids des panicules de F1 \ue9tait exceptionnellement diff\ue9rent de celui avec ou sans eau. F1 avait \ue9t\ue9 plus performant que P1, P2 et KM-4 avec 95.61, 146.37 et 328.81 % sans eau; et avec 155.44, 124. 27 et 82.02 % avec eau, respectivement. De diff\ue9rences significatives (P < 0.05) \ue9taient aussi r\ue9v\ue9l\ue9es entre les accessions de sorgho, le parent P2, les accessions KM-4 et les plants F1 \ue9tant tol\ue9rants. Ces r\ue9sultats indiquent que la tol\ue9rance multiple au stress peut augmenter la productivit\ue9 du sorgho

Cell membrane integrity, callose accumulation, and root growth in aluminum-stressed sorghum seedlings

Aluminum stress usually reduces plant root growth due to the accumulation of Al in specific zones of the root apex. The objectives of this study were to determine the localization of Al in the root apex of Sorghum bicolor (L.) Moech. and its effects on membrane integrity, callose accumulation, and root growth in selected cultivars. Seedlings were grown in a nutrient solution containing 0, 27, or 39 μM Al3+ for 24, 48, and 120 h. The Al stress significantly reduced root growth, especially after 48 and 120 h of exposure. A higher Al accumulation, determined by fluorescence microscopy after staining with a Morin dye, occurred in the root extension zone of the sensitive cultivar than in the tolerant cultivar. The membrane damage and callose accumulation were also higher in the sensitive than resistant cultivar. It was concluded that the Al stress significantly reduced root growth through the accumulation of Al in the root extension zone, callose accumulation, and impairment of plasma membrane integrity

Heterosis for yield and its components in sorghum (Sorghum bicolor L. Moench) hybrids in dry lands and sub-humid environments of East Africa

A study was conducted in 2011 and 2012 growing seasons to determine levels of heterosis and identify parents for use in sorghum hybrid production in East Africa. A total of 36 pairs of male sterile lines and 42 restorers were obtained from ICRISAT-Nairobi and used for generating 121 experimental hybrids in a line × tester mating design. The hybrids were then evaluated at Kiboko, Ukiriguru and Miwaleni locations in an alpha lattice design with three replications. Each genotype was grown in a 4 m long row at spacing of 60 cm ×50 cm. Phenotypic data were collected as per IPGRI, (1993) descriptors for sorghum on five randomly selected plants. There were significant differences among locations, crosses and male parents for all the characters studied. Female lines were highly significant for all traits except days to 50% flowering (DAF). Desired heterobeltiosis for DAF varied from -5.23 to -14% indicating of early maturing material that can escape terminal drought in rainfed agriculture, characteristic of East African cultivation system. Lowest (desired) heterobeltiosis for plant height was -53.61% with crosses ICSA15×Tegemeo and ATX623×KARI-MTAMA1most promising for this trait. Grain yield showed average heterosis and heterobeltiosis of up to 81.90% and 77.18% respectively both expressed in ICSA11×S35. The parents KARI MTAMA1, IESV91104DL, S35, BTX623, ICSB12 and ICSB11 produced hybrids that yielded high with medium height and maturity therefore could be included to develop hybrid sorghum for East Africa region

Combining ability of some sorghum lines for dry lands and sub-humid environments of East Africa

Sorghum (Sorghum bicolor L. Moench) is a major food crop grown in dry lands and sub-humid areas of East Africa. A study was conducted between 2010 to 2012 in dry lands (Miwaleni, Kiboko) and sub-humid (Ukiriguru) environments to identify parents for hybrid production. It involved 121 lines from ICRISAT and 121 hybrids developed from 36 male sterile lines and 42 restorer lines in a line × tester crossing. Experiments were planted in an alpha lattice design with three replications. Analysis revealed significant (P < 0.05) differences between parents and between crosses for yield and yield components, indicative of potentiality for exploitation. Line IESV23010 expressed best (-6.5) general combing ability (GCA) for days to 50% flowering (DAF). Highest general combiner for height was -55.4 expressed in ICSR24007 and for yield was 382.8 expressed in IESV92156DL. The crosses SDSA4×ICSR43 and SDSA4×ICSR59059 exhibited high and significant specific combining ability (SCA) for DAF. Lines IESB2 and ICSB44 were suited to sub-humid, whereas BTX623, ICSB15 and ICSB6 to dry lands environments. Testers IESV91104DL, IESV91131DL, ICSR93034 were well suited to dry lands whereas KARI-MTAMA1 and IESV23019 to sub-humid environments. The parents identified could be used to produce hybrids and varieties for the dry lands and sub-humid environments

EFFECT OF RIDGING AND INTERCROPPING ON SORGHUM PRODUCTIVITY IN ARID AND SEMI-ARID LANDS OF EASTERN KENYA

Soil moisture deficit is a key constraint to sorghum ( Sorghum bicolor ) productivity in arid and semi-arid lands globally. The objective of this study was to determine the effect of ridging and sorghum-bean intercropping (additive system) on soil moisture conservation and sorghum productivity. Sorghum (gadam) was grown either as a sole crop or intercropped with two bean ( Phaseolus vulgaris L.) varieties (KATx56 and KAT B1), under two types of ridging (open ridges and tied ridges), and a control without ridges for two years. The study was set up in split plot arrangement, in a randomised complete block design, at the Kenya Agricultural and Livestock Research Organization, Kiboko, in 2019 and 2020. There was no significant interaction between ridging and intercropping. Soil moisture content increased by 11-26% due to ridging; and decreased by -11 and -7% due to sorghum-KAT B1 and Sorghum-KAT X56 intercropping, respectively. Higher moisture content due to ridging was attributed to formation of basin-like structures, which increased water harvesting and infiltration compared to the no ridges where surface run-off was predominant. The highest moisture content was attained on sole bean, followed by sole sorghum and then sorghum/bean intercropping. The decrease in moisture content in intercrops of sorghum/bean relative to their specific sole crops was attributed to higher crop density, which reduced crop spacing, thus triggering competition for available soil moisture. The highest sorghum grain and equivalent yields were obtained in the ridged plots. Intercropping resulted into decrease in sorghum grain yield, but led to increase in sorghum equivalent yield (SEY) and Land Equivalent Ratio (LER). The results show that both ridging and intercropping are suitable for higher water use efficiency and land productivity in ASALs of Kenya.Le d\ue9ficit d\u2019humidit\ue9 du sol est une contrainte majeure \ue0 la productivit\ue9 du sorgho (Sorghum bicolor) dans les terres arides et semi-arides \ue0 l\u2019\ue9chelle mondiale. L\u2019objectif de cette \ue9tude \ue9tait de d\ue9terminer l\u2019effet du billonnage et de la culture intercalaire sorgho-haricot (syst\ue8me additif) sur la conservation de l\u2019humidit\ue9 du sol et la productivit\ue9 du sorgho. Le sorgho (gadam) \ue9tait cultiv\ue9 soit en monoculture, soit en association avec deux vari\ue9t\ue9s de haricot (Phaseolus vulgaris L.) (KATx56 et KAT B1), sous deux types de billons (billons ouverts et billons li\ue9s), et un t\ue9moin sans billons pendant deux ann\ue9es. L\u2019\ue9tude a \ue9t\ue9 mise en place en parcelles divis\ue9es, dans une conception en blocs complets randomis\ue9s, \ue0 l\u2019Organisation de recherche sur l\u2019agriculture et l\u2019\ue9levage du Kenya, Kiboko, en 2019 et 2020. L\u2019\ue9tude n\u2019a montr\ue9 aucune interaction significative entre le billonnage et la culture intercalaire. La teneur en humidit\ue9 du sol a augment\ue9 de 11 \ue0 26 % en raison du billonnage ; et diminu\ue9 de -11 et -7% en raison des cultures intercalaires sorgho-KAT B1 et Sorgho-KAT X56, respectivement. La teneur en humidit\ue9 plus \ue9lev\ue9e due aux cr\ueates a \ue9t\ue9 attribu\ue9e \ue0 la formation de structures de type bassin, ce qui a augment\ue9 la collecte et l\u2019infiltration de l\u2019eau par rapport \ue0 l\u2019absence de cr\ueates o\uf9 le ruissellement de surface \ue9tait pr\ue9dominant. La teneur en humidit\ue9 la plus \ue9lev\ue9e \ue9tait sur le haricot unique, suivi du sorgho unique, puis de la culture intercalaire sorgho/haricot. La diminution de la teneur en humidit\ue9 dans les cultures intercalaires de sorgho/haricot par rapport \ue0 leurs cultures uniques sp\ue9cifiques a \ue9t\ue9 attribu\ue9e \ue0 une densit\ue9 de culture plus \ue9lev\ue9e, qui a r\ue9duit l\u2019espacement des cultures, d\ue9clenchant une comp\ue9tition pour l\u2019humidit\ue9 disponible du sol. Les rendements les plus \ue9lev\ue9s en grain de sorgho et en \ue9quivalent ont \ue9t\ue9 obtenus dans les parcelles butt\ue9es. La culture intercalaire a entra\ueen\ue9 une diminution du rendement en grains de sorgho, mais a entra\ueen\ue9 une augmentation du rendement \ue9quivalent en sorgho (SEY) et du rapport d\u2019\ue9quivalent en terres (LER). Les r\ue9sultats montrent que le billonnage et la culture intercalaire conviennent \ue0 une plus grande efficacit\ue9 de l\u2019utilisation de l\u2019eau et \ue0 la productivit\ue9 des terres dans les TASA du Kenya

Phylogenetic relationship among Kenyan sorghum germplasms based on aluminium tolerance

Eighty nine (89) sorghum lines sourced from various parts of Kenya were used to determine phylogenetic relationships based on 10 DNA fragments at AltSB loci with SbMATE, ORF9 and MITE primers. Nine lines of varying aluminium tolerance levels were selected to compare their SbMATE gene expression via the real-time PCR quantification of SbMATE gene expression. The sorghum line MSCR O2 expressed a thousandfold more SbMATEgene activity than the sensitive lines (MSCRM49, MSCRN84 and MSCRN61) under Al treatment. Analysis was done by agarose gel electrophoresis stained with ethidium bromide. The objective of this study was to assess the level of phylogenetic relationships among the Kenyan sorghum germplasms at a known Al tolerance locus. Hierarchical cluster analysis joined at 70% simple matching coefficient using average linkage similarity level produced nine groups in which 67 lines fell in three major clusters of 39, 15 and 13 lines each. The three Al tolerant lines MSCRO2, MSCRC1 and MSCRN60 were clustered together. Lines MSCRO2, MSCRC1 and MSCRN60, screened to be Al tolerant were genetically related at 70% average linkage similarity level and therefore recommend their further development as a food security measure in Kenya.Keywords: Aluminium (Al) toxicity, Sorghum bicolor, Sorghum bicolor multi-drug, toxic extrusion compound (SbMATE) gene expressionAfrican Journal of Biotechnology Vol. 12(22), pp. 3528-353