TELEX HEBDOMADAIRE NR 95 DU 17.09.82 DESTINE A L'ENSEMBLE DES DELEGATIONS EXTERIEURES ET BUREAUX DE PRESS ET D'INFORMATION INDEPENDANTS DANS LES PAYS TIERS = WEEKLY MEMO NO. 95 FOR 17.09.82 TO FOREIGN DELEGATIONS AND PRESS BUREAUS OF THIRD COUNTRIES

<p>High-performance liquid chromatography (HPLC) results of (A) commercial surfactin sample, and (B) our extract surfactin of <i>B</i>. <i>subtilis</i> HH2 in LB medium. There were three main peaks (Peak A-C) of the extract and the surfactin standard in the same location.</p

Characterization of 16 specimens based on multi-locus sequences of <i>bg</i>, <i>tpi</i> and <i>gdh</i> genes.

<p>Characterization of 16 specimens based on multi-locus sequences of <i>bg</i>, <i>tpi</i> and <i>gdh</i> genes.</p

Anti-NDV activity of 9-oxo10,11-dehydroageraphorone extracted from <i>Eupatorium adenophorum</i> Spreng <i>in vitro</i>

<p>The purpose of this study was to investigate the anti-Newcastle disease virus (NDV) activities of 9-oxo-10,11-dehydroageraphorone (euptox A) from <i>Eupatorium adenophorum</i> Spreng (<i>E. adenophorum</i>) <i>in vitro</i>. NDV infection of chicken embryo fibroblasts (CEFs) was performed. Cytotoxicities and antiviral activities of euptox A was assessed by the MTT method. The interaction of NDV with cell membrane protein was detected by virus overlay protein binding assay (VOPBA). The expression levels of NDV genes in CEFs was tested by RTFQ PCR. The results showed that the maximal safe concentrations of euptox A to CEFs was 10 μg/mL. Euptox A could directly neutralise NDV, inhibit the infectivity of NDV to CEFs and block intracellular NDV treat NDV infection. And euptox A brings competitiveness inhibition for NDV binding to its receptors and then prevent NDV infection. These results indicated that euptox A possessed anti-NDV activity has potential use as components of a natural antiviral drug.</p

Sodium selenite inhibits deoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture.zip

oxidative stress biomarker response

Molecular characterization and multi-locus genotypes of <i>Enterocytozoon bieneusi</i> from captive red kangaroos (<i>Macropus Rfus</i>) in Jiangsu province, China

<div><p><i>Enterocytozoon bieneusi</i> is the most common pathogen of microsporidian species infecting humans worldwide. Although <i>E</i>. <i>bieneusi</i> has been found in a variety of animal hosts, information on the presence of <i>E</i>. <i>bieneusi</i> in captive kangaroos in China is limited. The present study was aimed at determining the occurrence and genetic diversity of <i>E</i>. <i>bieneusi</i> in captive kangaroos. A total of 61 fecal specimens (38 from red kangaroos and 23 from grey kangaroos) were collected from Nanjing Hongshan Forest Zoo and Hongshan Kangaroo Breeding Research Base, Jiangsu province, China. Using the nested PCR amplification ITS gene of rRNA of <i>E</i>. <i>bieneusi</i>, totally 23.0% (14/61) of tested samples were PCR-positive with three genotypes (i.e. one known genotype, CHK1, and two novel genotypes, CSK1 and CSK2). Multi-locus sequence typing using three microsatellites (MS1, MS3, and MS7) and one minisatellite (MS4) revealed one, five, two, and one types at these four loci, respectively. In phylogenetic analysis, the two genotypes, CHK1 and CSK1, were clustered into a new group of unknown zoonotic potential, and the novel genotype CSK2 was clustered into a separate clade with PtEb and PtEbIX. To date, this is the first report on the presence of <i>E</i>. <i>bieneusi</i> in captive red kangaroos in Jiangsu province, China. Furthermore, a high degree of genetic diversity was observed in the <i>E</i>. <i>bieneusi</i> genotype and seven MLGs (MLG1-7) were found in red kangaroos. Our findings suggest that infected kangaroo may act as potential reservoirs of <i>E</i>. <i>bieneusi</i> and be source to transmit infections to other animal.</p></div

Phylogenetic relationship among the ITS loci of <i>E</i>.<i>bieneusi</i> isolates.

<p>Phylogenetic relationship of the genotypes of <i>E</i>. <i>bieneusi</i> identified in this study and known genotypes previously published in GenBank as inferred by a neighbor-joining analysis of ITS sequences based on genetic distances calculated by the Kimura 2-parameter model. A similar topology tree was also performed by maximum parsimony analysis, with the exception that the CSK2 genotype grouped together with genotypes PtEb and PtEbIX, with 99% bootstrap value. The numbers on the branches are percent bootstrapping values from 1000 replicates, with more than 50% shown in the tree. Each sequence is identified by its accession number, genotype designation, and host origin. Genotypes marked with black circles and open circle are novel and known genotypes identified in this study, respectively.</p

Phylogenetic relationship of <i>Giardia duodenalis</i> assemblage B MLGs inferred by the neighbor-joining analysis of concatenated <i>bg</i>, <i>tpi</i> and <i>gdh</i> sequences.

<p>Bootstrap values greater than 50% from 1000 replicates are shown. Concatenated sequences from this study are marked by filed roundness.</p

Characterization of 16 specimens based on multi-locus sequences of <i>bg</i>, <i>tpi</i> and <i>gdh</i> genes.

<p>Characterization of 16 specimens based on multi-locus sequences of <i>bg</i>, <i>tpi</i> and <i>gdh</i> genes.</p

Prevalence and distribution of <i>E</i>. <i>bieneusi</i> genotypes in Nanjing Hongshan Forest Zoo and Hongshan Kangaroo Breeding Research Base.

<p>Prevalence and distribution of <i>E</i>. <i>bieneusi</i> genotypes in Nanjing Hongshan Forest Zoo and Hongshan Kangaroo Breeding Research Base.</p