Indoor Calibration using Segment Chains

International audienceIn this paper, we present a new method for line segments matching for indoor reconstruction. Instead of matching individual seg- ments via a descriptor like most methods do, we match segment chains that have a distinctive topology using a dynamic programing formulation. Our method relies solely on the geometric layout of the segment chain and not on photometric or color profiles. Our tests showed that the presented method is robust and manages to produce calibration information even under a drastic change of viewpoint

Deterioro del aceite durante el almacenamiento de los hígados de merluza en comparación con el del aceite extraído de ellos

Hake liver (Merluccis hubbsi) is a by-product of the fillet industry and is used as a source of oil . In the present paper the oil content of livers stored at 4ºC and -20ºC is studied compared to that of the oil extracted from livers and stored under the same conditions. Oil contained in livers stored at -20ºC was oxidized in two weeks; the oil extracted from livers and stored under the same conditions was preserved for several months. The problem is that once the oil becomes deteriorated in livers it is not possible to stabilize it by adding antioxidants. Thus it is necessary to store livers at temperatures lower than -20ºC, to extract the oil as soon as possible, to add the correct antioxidants and to store it under refrigeration, in order to obtain a high quality oil.Los hígados de merluza (Merluccius hubbsi) son un subproducto de la industria fileteadora y se utilizan como fuente de aceite. En este trabajo se estudia el aceite contenido en dichos hígados durante su almacenamiento a 4ºC y a -20ºC frente a la del aceite extraído de ellos y guardado en iguales condiciones. El aceite contenido en los hígados almacenados a -20ºC, se oxida en un par de semanas; el aceite extraído de ellos y almacenado en iguales condiciones se conserva durante varios meses. El problema principal radica en que, una vez que el aceite se deteriora en los hígados, no es posible estabilizarlo por el agregado de antioxidantes. Para obtener un aceite de buena calidad es imprescindible que los hígados se almacenen a una temperatura menor de -20ºC, que su aceite se extraiga lo antes posible, que se le adicione de antioxidantes adecuados y que se le almacene refrigerad

Caracterización de grasas para caldos deshidratados

Fats represent a major part of the weight of dehydrated soup cubes. As these products are stored at room temperature, the fatty components should show no partial melting during the Summer months. Six fatty raw materials, mixtures of these fats already prepared by the manufacturer and lipidic extracts from the finished cubes (both hen and vegetables soups) are analyzed in this work, trying to relate origin (fatty acid composition) with stability against temperature changes (DSC thermogram). Some of the studied fats are found acceptable according to the expected shelf life of the product, although others should be modified before usage, either by fractioning or by hydrogenation.Las grasas constituyen un alto porcentaje del peso de los caldos deshidratados envasados bajo forma de cubitos. Como estos alimentos se almacenan a temperatura ambiente, sus componentes grasos no deben fundir parcialmente durante el verano. En este trabajo se analizan seis muestras de materias primas grasas, mezclas de ellas ya preparadas por la empresa elaboradora y extractos lipidióos del producto terminado (sopas de gallina y de verduras). Se busca relacionar la naturaleza de las grasas (composición en ácidos grasos) con su estabilidad frente a variaciones de la temperatura (termogramas por DSC). Se concluye que, si bien algunas de las grasas estudiadas son aceptables desde el punto de vista de la vida útil esperada del producto, otras se deberían modificar por hidrogenación o fraccionamiento antes de su uso

MRI-targeted or standard biopsy for prostate-cancer diagnosis

Background Multiparametric magnetic resonance imaging (MRI), with or without targeted biopsy, is an alternative to standard transrectal ultrasonography-guided biopsy for prostate-cancer detection in men with a raised prostate-specific antigen level who have not undergone biopsy. However, comparative evidence is limited. Methods In a multicenter, randomized, noninferiority trial, we assigned men with a clinical suspicion of prostate cancer who had not undergone biopsy previously to undergo MRI, with or without targeted biopsy, or standard transrectal ultrasonography-guided biopsy. Men in the MRI-targeted biopsy group underwent a targeted biopsy (without standard biopsy cores) if the MRI was suggestive of prostate cancer; men whose MRI results were not suggestive of prostate cancer were not offered biopsy. Standard biopsy was a 10-to-12-core, transrectal ultrasonography-guided biopsy. The primary outcome was the proportion of men who received a diagnosis of clinically significant cancer. Secondary outcomes included the proportion of men who received a diagnosis of clinically insignificant cancer. Results A total of 500 men underwent randomization. In the MRI-targeted biopsy group, 71 of 252 men (28%) had MRI results that were not suggestive of prostate cancer, so they did not undergo biopsy. Clinically significant cancer was detected in 95 men (38%) in the MRI-targeted biopsy group, as compared with 64 of 248 (26%) in the standard-biopsy group (adjusted difference, 12 percentage points; 95% confidence interval [CI], 4 to 20; P=0.005). MRI, with or without targeted biopsy, was noninferior to standard biopsy, and the 95% confidence interval indicated the superiority of this strategy over standard biopsy. Fewer men in the MRI-targeted biopsy group than in the standard-biopsy group received a diagnosis of clinically insignificant cancer (adjusted difference, -13 percentage points; 95% CI, -19 to -7; P<0.001). Conclusions The use of risk assessment with MRI before biopsy and MRI-targeted biopsy was superior to standard transrectal ultrasonography-guided biopsy in men at clinical risk for prostate cancer who had not undergone biopsy previously. (Funded by the National Institute for Health Research and the European Association of Urology Research Foundation; PRECISION ClinicalTrials.gov number, NCT02380027 .)

Filling an Emulsion Drop with Motile Bacteria

We have measured the spatial distribution of motile Escherichia coli inside spherical water droplets emulsified in oil. At low cell concentrations, the cell density peaks at the water-oil interface; at increasing concentration, the bulk of each droplet fills up uniformly while the surface peak remains. Simulations and theory show that the bulk density results from a `traffic' of cells leaving the surface layer, increasingly due to cell-cell scattering as the surface coverage rises above $\sim 10\%$. Our findings show similarities with the physics of a rarefied gas in a spherical cavity with attractive walls.Comment: 5 pages, 4 figures, Supporting Information (5 pages, 5 figures

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks

RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which occurs at inactivated replication forks by the annealing of blocked leading and lagging strand ends. RuvAB was recently proposed to bind replication forks and directly catalyze their conversion into HJs. We report here the isolation and characterization of two separation-of-function ruvA mutants that resolve HJs, based on their capacity to promote conjugational recombination and recombinational repair of UV and mitomycin C lesions, but have lost the capacity to reverse forks. In vivo and in vitro evidence indicate that the ruvA mutations affect DNA binding and the stimulation of RuvB helicase activity. This work shows that RuvA's actions at forks and at HJs can be genetically separated, and that RuvA mutants compromised for fork reversal remain fully capable of homologous recombination

Replication Fork Reactivation in a dnaC2 Mutant at Non-Permissive Temperature in Escherichia coli

Replicative helicases unwind double-stranded DNA in front of the polymerase and ensure the processivity of DNA synthesis. In Escherichia coli, the helicase loader DnaC as well as factors involved in the formation of the open complex during the initiation of replication and primosomal proteins during the reactivation of arrested replication forks are required to recruit and deposit the replicative helicase onto single-stranded DNA prior to the formation of the replisome. dnaC2 is a thermosensitive allele of the gene specifying the helicase loader; at non-permissive temperature replication cannot initiate, but most ongoing rounds of replication continues through to completion (18% of dnaC2 cells fail to complete replication at non-permissive temperature). An assumption, which may be drawn from this observation, is that only a few replication forks are arrested under normal growth conditions. This assumption, however, is at odds with the severe and deleterious phenotypes associated with a null mutant of priA, the gene encoding a helicase implicated in the reactivation of arrested replication forks. We developed an assay that involves an abrupt inactivation of rounds of synchronized replication in a large population of cells, in order to evaluate the ability of dnaC2 cells to reactivate arrested replication forks at non-permissive temperature. We compared the rate at which arrested replication forks accumulated in dnaC2 priA+ and dnaC2 priA2 cells and observed that this rate was lower in dnaC2 priA+ cells. We conclude that while replication cannot initiate in a dnaC2 mutant at non-permissive temperature, a class of arrested replication forks (PriA-dependent and DnaC-independent) are reactivated within these cells