689 research outputs found
Association of Fruit and Vegetable Intake with Cardiorespiratory Fitness in Adolescents
An association has been established between total dietary energy intake and cardiorespiratory fitness (CRF) in adolescents. However, there is little research examining specific dietary components, such as fruit and vegetable intake. PURPOSE: The primary purpose of this research is to determine if an association exists between fruit and vegetable (F/V) intake and CRF in adolescents. A secondary purpose of this study is to determine if an association exists between F/V intake and body mass index (BMI). METHODS: A sample of 424 adolescents (234 males and 190 females), age 10-18 years, completed the Dietary Behavior section of the Youth Risk Behavior Survey (YRBS) and the FITNESSGRAM 20-meter Pacer test (PACER). Height and weight were also measured to determine BMI. This section of the YRBS assesses F/V intake based on intake frequency over a one-week period. Peak oxygen consumption (VO2peak) was estimated from the PACER results and categorized based on the FITNESSGRAM aerobic standards, placing individuals into one of three categories: Healthy Fitness Zone (HFZ), Needs Improvement (NI), and Needs Improvement – Health Risk (NI-HR). Body composition was estimated using Body Mass Index (BMI), which was calculated from participants’ height and weight, and categorized based on the FITNESSGRAM BMI standards, placing individuals into one of four categories: Very Lean (VL), HFZ, NI, and NI-HR. Mean differences in total F/V intake for participants in each of the CRF and BMI categories were assessed using a one-way ANOVA. RESULTS: The mean total F/V (F/V) intake values (times per week) showed slight differences between each of the categories. For male participants the F/V intake values in the HFZ, NI, and NI-HR categories were 19.9 (SD 15.2), 15.8 (SD 19.2), and 19.1 (SD 13.8) respectively. The mean F/V intake for female participants in the HFZ, NI, and NI-HR categories were 20.9 (SD 16.2), 20.3 (SD 19.6), and 15.9 (SD 9.6) respectively. However, none of these differences were statistically significant (all p\u3e0.05). Average F/V intakes were also individually analyzed, but with similar results (all p\u3e0.05). Similar results were found for mean F/V intake between each of the BMI categories. F/V intake for male participants in the VL, HFZ, NI, and NI-HR categories were 19.6 (SD 13.1), 18.9 (SD 16.1), 20.3 (SD 14.6), and 17.5 (SD 18.1) respectively. The mean F/V intake for females in the VL, HFZ, NI, and NI-HR categories were 25.6 (SD 16.1), 19.6 (SD 15.2), 24.5 (SD 22.1), and 15.4 (SD 11.6) respectively. Likewise, none of these differences were significant (all p\u3e.05). CONCLUSION: F/V intake does not have a significant association with CRF or BMI values in adolescents
Analysis of homotypic and heterotypic serum immune responses to rotavirus proteins following primary rotavirus infection by using the radioimmunoprecipitation technique
This New Ocean: A History of Project Mercury
When Congress created the National Aeronautics and Space Administration (NASA) in 1958, it charged NASA with the responsibility "to contribute materially to . . . the expansion of human knowledge of phenomena in the atmosphere and space" and "provide for the widest practicable and appropriate dissemination of information concerning its activities and the results thereof." NASA wisely interpreted this mandate to include responsibility for documenting the epochal progress of which it is the focus. The result has been the development of a historical program by NASA as unprecedented as the task of extending man's mobility beyond his planet. This volume is not only NASA's accounting of its obligation to disseminate information to our current generation of Americans. It also fulfills, as do all of NASA's future-oriented scientific-technological activities, the further obligation to document the present as the heritage of the future. The wide-ranging NASA history program includes chronicles of day-to-day space activities; specialized studies of particular fields within space science and technology; accounts of NASA's efforts in organization and management, where its innovations, while less known to the public than its more spectacular space shots, have also been of great significance; narratives of the growth and expansion of the space centers throughout the country, which represent in microcosm many aspects of NASA's total effort; program histories, tracing the successes- and failures- of the various projects that mark man's progress into the Space Age; and a history of NASA itself, incorporating in general terms the major problems and challenges, and the responses thereto, of our entire civilian space effort. The volume presented here is a program history, the first in a series telling of NASA's pioneering steps into the Space Age. It deals with the first American manned-spaceflight program: Project Mercury. Although some academicians might protest that this is "official" history, it is official only in the fact that it has been prepared and published with the support and cooperation of NASA. It is not "official" history in the sense of presenting a point of view supposedly that of NASA officialdom-if anyone could determine what the "point of view" of such a complex organism might be. Certainly, the authors were allowed to pursue their task with the fullest freedom and in accordance with the highest scholarly standards of the history profession
An Integrated Content and Metadata based Retrieval System for Art
In this paper we describe aspects of the Artiste project to develop a distributed content and metadata based analysis, retrieval and navigation system for a number of major European Museums. In particular, after a brief overview of the complete system, we describe the design and evaluation of some of the image analysis algorithms developed to meet the specific requirements of the users from the museums. These include a method for retrievals based on sub images, retrievals based on very low quality images and retrieval using craquelure type
London governance and the politics of neighbourhood planning: a case for investigation
The Localism Act 2011 has successfully devolved planning powers to over 2,500 English communities, involving 14 million people, with over 700 ‘made’ neighbourhood plans legitimised by referendum. In London, however, there are less than one-tenth of the made plans than in the rest of England. Institutional resistance and policy choices may be implicated. Two national studies of neighbourhood planning are reviewed. The role of the local authority is found to be a crucial factor in determining progress, and issues of social deprivation and unequal access are highlighted. Theorisation is considered by reference to a range of academic studies of localism and neighbourhood planning. Distinctions made between ‘representative’ and ‘community’ localism, and objections to anti-political effects, are noted. There has been remarkably little research into borough governance and neighbourhood planning in the capital. Based upon evidence of anomalous and differentiated governance practice, a study in London is called for
‘If independence goes, the planning system goes’: New Political Governance and the English Planning Inspectorate
Radical restructuring of 'arms-length' government bodies following the 2010 UK national election signalled a change in relations between government and the civil service. This was seen as a major shift in modes of governance from 'new public management' to a more politicised mode of 'new political governance'. This paper presents an analysis of the impacts of these shifts on the English Planning Inspectorate, an executive agency central to the land-use planning system. It identifies measures by ministers to increase control over the Inspectorate that represent a shift in governance culture and a shift in the planning system itself
Evidence of false-positive results in a commercially available rotavirus assay in the vaccine era, Australia, 2011 to 2012
Concerns were raised about specificity of the VIKIA Rota-Adeno immunochromatographic kit. Only 28-37% of samples positive with the VIKIA kit could be confirmed using two real-time RT-PCR assays and three ELISA kits. On re-analysis of a subset of the positive samples, 86% remained positive with the VIKIA kit, however, 90% remained negative in the other assays. In a highly vaccinated population we found a high number of false-positive rotavirus tests with a widely-used commercial kit
The point prevalence of respiratory syncytial virus in hospital and community-based studies in children from Northern Australia:studies in a 'high-risk' population
Introduction: Respiratory syncytial virus (RSV) is the leading viral cause of acute lower respiratory infections globally, accounting for high morbidity and mortality burden among children aged less than 5 years. As candidate RSV vaccine trials in pregnant women and infants are underway a greater understanding of RSV epidemiology is now needed, especially in paediatric populations with high rates of acute and chronic respiratory disease. The objective was to identify RSV prevalence in children living in northern Australia, a region with a high respiratory disease burden. Methods: Data were sourced from 11 prospective studies (four hospital and seven community-based) of infants and children with acute and chronic respiratory illnesses, as well as otitis media, conducted between 1996 and 2017 inclusive. The data from northern Australian children in these trials were extracted and, where available and consented, their nasopharyngeal swabs (biobanked at -80°C) were tested by polymerase chain reaction assays for RSV-A and B, 16 other viruses and atypical respiratory bacterial pathogens. Results: Overall, 1127 children were included. Their median age was 1.8 years (interquartile range 0.5-4.9); 58% were male and 90% Indigenous, with 81% from remote communities. After human rhinoviruses (HRV), RSV was the second most prevalent virus (15%, 95% confidence interval (CI) 13-18). RSV prevalence was greatest amongst children aged less than 2 years hospitalised with bronchiolitis (47%, 95%CI 41.4-52.4), with more than two-thirds with RSV aged less than 6 months. In contrast, the prevalence of RSV was only 1-3.5% in other age groups and settings. In onethird of RSV cases, another respiratory virus was also detected. Individual viruses other than RSV and HRV were uncommon (0-9%). Conclusion: Combined data from 11 hospital and communitybased studies of children aged less than 18 years who lived in communities with a high burden of acute and chronic respiratory illness showed that RSV was second only to HRV as the most prevalent virus detected across all settings. RSV was the most frequently detected virus in infants hospitalised with bronchiolitis, including those aged less than 6 months. In contrast, RSV was uncommonly detected in children in community settings. In northern Australia, effective maternal and infant RSV vaccines could substantially reduce RSV bronchiolitis-related hospitalisations, including admissions of Indigenous infants from remote communities.</p
Comparison of test specificities of commercial antigen-based assays and in-house PCR methods for detection of rotavirus in stool specimens
Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one commercial ICT, all the assays were generally reliable for rotavirus detection
Integrating microarray analysis and the soybean genome to understand the soybeans iron deficiency response
<p>Abstract</p> <p>Background</p> <p>Soybeans grown in the upper Midwestern United States often suffer from iron deficiency chlorosis, which results in yield loss at the end of the season. To better understand the effect of iron availability on soybean yield, we identified genes in two near isogenic lines with changes in expression patterns when plants were grown in iron sufficient and iron deficient conditions.</p> <p>Results</p> <p>Transcriptional profiles of soybean (<it>Glycine max</it>, L. Merr) near isogenic lines Clark (PI548553, iron efficient) and IsoClark (PI547430, iron inefficient) grown under Fe-sufficient and Fe-limited conditions were analyzed and compared using the Affymetrix<sup>® </sup>GeneChip<sup>® </sup>Soybean Genome Array. There were 835 candidate genes in the Clark (PI548553) genotype and 200 candidate genes in the IsoClark (PI547430) genotype putatively involved in soybean's iron stress response. Of these candidate genes, fifty-eight genes in the Clark genotype were identified with a genetic location within known iron efficiency QTL and 21 in the IsoClark genotype. The arrays also identified 170 single feature polymorphisms (SFPs) specific to either Clark or IsoClark. A sliding window analysis of the microarray data and the 7X genome assembly coupled with an iterative model of the data showed the candidate genes are clustered in the genome. An analysis of 5' untranslated regions in the promoter of candidate genes identified 11 conserved motifs in 248 differentially expressed genes, all from the Clark genotype, representing 129 clusters identified earlier, confirming the cluster analysis results.</p> <p>Conclusion</p> <p>These analyses have identified the first genes with expression patterns that are affected by iron stress and are located within QTL specific to iron deficiency stress. The genetic location and promoter motif analysis results support the hypothesis that the differentially expressed genes are co-regulated. The combined results of all analyses lead us to postulate iron inefficiency in soybean is a result of a mutation in a transcription factor(s), which controls the expression of genes required in inducing an iron stress response.</p
- …