Barriers to Counseling Among Human Service Professionals: The Development and Validation of the Fit, Stigma, & Value Scale

This study sought to confirm rates of attendance in counseling of human service professionals and validate a 32-item questionnaire designed to identify barriers to counseling seeking behavior among this population. Results indicated that a large percentage of human service professionals attend counseling, with males and females attending at similar rates and non-Caucasians attending at lower rates. A multivariate analysis of variance and descriptive statistics identified the most common barriers to attendance in counseling and examined demographic differences in participants’ sensitivity towards barriers to attendance in counseling. A Principal Factor Analysis (PFA) revealed three subscales (fit, value, and stigma), which we called the Fit, Stigma, & Value (FSV) Scale. How the instrument can be used with students in human service programs, and with human service professionals, to reduce barriers to attendance in counseling and ultimately ameliorate personal problems, reduce vicarious traumatization, and limit countertransference are discussed

A Study of the Unionidae of Tinkers Creek, Ohio

We present data on freshwater mussel (Mollusca: Bivalvia: Unionidae) distributions for Tinkers Creek, a small Ohio stream that previously had not been surveyed for its unionid fauna. The distribution of these mussels was mapped against the changing habitat of the stream, with special attention paid to two zones of human manipulation, a wastewater treatment plant and a stretch of the river where the bed was relocated to enable development. At least five unionid species live in Tinkers Creek. These are Pyganodon grandis, Lampsilis radiata luteola, Fusconaiajlava, Lasmigona costata, and Lasmigona compressa. Fresh shells suggested the presence of three others: Strophitus undulatus, Toxolasma parvus, and Alasmidonta marginata. The region of Tinkers Creek where the mussels are most common extends through Twinsburg, Ohio, and upstream to a waterfall just below the confluence with Pond Brook. Relocation of the river channel eliminated mussels from a 0.5 km stretch in eastern Twinsburg. Furthermore, the impact of effluent from a wastewater treatment plant was minor, at most. Mussel diversity diminished from five to three species commonly found below this facility. A change in river habitat to faster flow, however, provided an alternate explanation for this faunal change. The most striking difference was the replacement of Lasmigona costata, a species usually found in medium and large rivers, by L. compressa, a species common in small streams and headwaters

A Study of the Unionidae of Tinkers Creek, Ohio

We present data on freshwater mussel (Mollusca: Bivalvia: Unionidae) distributions for Tinkers Creek, a small Ohio stream that previously had not been surveyed for its unionid fauna. The distribution of these mussels was mapped against the changing habitat of the stream, with special attention paid to two zones of human manipulation, a wastewater treatment plant and a stretch of the river where the bed was relocated to enable development. At least five unionid species live in Tinkers Creek. These are Pyganodon grandis, Lampsilis radiata luteola, Fusconaiajlava, Lasmigona costata, and Lasmigona compressa. Fresh shells suggested the presence of three others: Strophitus undulatus, Toxolasma parvus, and Alasmidonta marginata. The region of Tinkers Creek where the mussels are most common extends through Twinsburg, Ohio, and upstream to a waterfall just below the confluence with Pond Brook. Relocation of the river channel eliminated mussels from a 0.5 km stretch in eastern Twinsburg. Furthermore, the impact of effluent from a wastewater treatment plant was minor, at most. Mussel diversity diminished from five to three species commonly found below this facility. A change in river habitat to faster flow, however, provided an alternate explanation for this faunal change. The most striking difference was the replacement of Lasmigona costata, a species usually found in medium and large rivers, by L. compressa, a species common in small streams and headwaters

Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3.

The crystal structure of a conserved domain of nonstructural protein 3 (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has been solved by single-wavelength anomalous dispersion to 1.4 A resolution. The structure of this "X" domain, seen in many single-stranded RNA viruses, reveals a three-layered alpha/beta/alpha core with a macro-H2A-like fold. The putative active site is a solvent-exposed cleft that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is similar to yeast YBR022W (also known as Poa1P), a known phosphatase that acts on ADP-ribose-1''-phosphate (Appr-1''-p). The SARS nsP3 domain readily removes the 1'' phosphate group from Appr-1''-p in in vitro assays, confirming its phosphatase activity. Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1''-p

Clinical Utility of Serum Autoantibodies Detected by Protein Microarray in Melanoma

Better prognostic and predictive markers in melanoma are needed to select patients for therapy. We utilized a dual-lectin affinity chromatography and a natural protein microarray-based analysis to select a subproteome of target glycoproteins to profile serum antibodies against melanoma associated antigens that may predict nodal positivity. We identified 5 melanoma-associated antigens using this microarray coupled to mass spectrometry; GRP75, GRP94, ASAH1, CTSD and LDHB. We evaluated their predictive value for nodal status adjusting for age, gender, Breslow thickness, mitotic rate and ulceration using standard logistic regression. After adjustment, ASAH1, CTSD and LDHB were significantly negatively associated with nodal status (P = 0.0008) and GRP94 was significantly positively associated (P = 0.014). Our best multivariate model for nodal positivity included Breslow thickness, presence of serum anti-ASAH1, anti-LDHB or anti-CTSD, and presence of serum anti-GRP94, with an area under the ROC curve of 0.869. If validated, these results show promise for selecting clinically node negative patients for SLN biopsy. In addition, there is strong potential for glycoprotein microarray to screen serum autoantibodies that may identify patients at high risk of distant metastases or those likely or unlikely to respond to treatment, and these proteins may serve as targets for intervention

Rapid Fabrication of Flat Plate Cavity Phosphor Thermography Test Models for Shuttle Return-to-Flight Aero-Heating

Methods, materials and equipment are documented for fabricating flat plate test models at NASA Langley Research Center for Shuttle return-to-flight aeroheating experiments simulating open and closed cavity interactions in Langley s hypersonic 20-Inch Mach 6 air wind tunnel. Approximately 96 silica ceramic flat plate cavity phosphor thermography test models have been fabricated using these methods. On one model, an additional slot is machined through the back of the plate and into the cavity and vented into an evacuated plenum chamber to simulate a further opening in the cavity. After sintering ceramic to 2150 F, and mounting support hardware, a ceramic-based two-color thermographic phosphor coating is applied for global temperature and heat transfer measurements, with fiducial markings for image registration

Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment

BACKGROUND: Interactions between immune and tumor cells are critical to determining cancer progression and response. In addition, preclinical prediction of immune-related drug efficacy is limited by interspecies differences between human and mouse, as well as inter-person germline and somatic variation. To address these gaps, we developed an autologous system that models the tumor microenvironment (TME) from individual patients with solid tumors. METHOD: With patient-derived bone marrow hematopoietic stem and progenitor cells (HSPCs), we engrafted a patient\u27s hematopoietic system in MISTRG6 mice, followed by transfer of patient-derived xenograft (PDX) tissue, providing a fully genetically matched model to recapitulate the individual\u27s TME. We used this system to prospectively study tumor-immune interactions in patients with solid tumor. RESULTS: Autologous PDX mice generated innate and adaptive immune populations; these cells populated the TME; and tumors from autologously engrafted mice grew larger than tumors from non-engrafted littermate controls. Single-cell transcriptomics revealed a prominent vascular endothelial growth factor A (VEGFA) signature in TME myeloid cells, and inhibition of human VEGF-A abrogated enhanced growth. CONCLUSIONS: Humanization of the interleukin 6 locus in MISTRG6 mice enhances HSPC engraftment, making it feasible to model tumor-immune interactions in an autologous manner from a bedside bone marrow aspirate. The TME from these autologous tumors display hallmarks of the human TME including innate and adaptive immune activation and provide a platform for preclinical drug testing