Stress and Dyadic Coping in Personal Projects of Couples – A Pattern-Oriented Analysis

Relational accounts of goal striving have barely considered dyadic coping as an element of the process, nor has dyadic coping research utilized the unique advantages of the goal construct (e.g., in form of personal project assessment) so far. Therefore, the primary aim of the present study was to explore stress and dyadic coping experiences associated with the personal projects of partners in a close relationship. Moreover, we approached data analysis in a pattern-oriented way, instead of using variable-centered linear models. We used cross-sectional data from 270 married and cohabiting Hungarian heterosexual couples (mean age 40.1 ± 11.2 and 37.8 ± 10.9 years for male and female partners, respectively). Partners individually completed an adapted version of the Personal Project Assessment procedure. First, they named an important but stressful personal project. Respondents appraised their experiences with the chosen personal project along several predefined aspects. These included: (1) stress experiences; (2) dyadic coping, using the adapted Dyadic Coping Inventory; (3) positive emotions; and (4) sense of community. The Relationship Assessment Scale was also assessed. Cluster analysis of both partners’ stress experiences, positive and negative dyadic coping strategies in their own personal projects revealed six relationship-level clusters. Cluster solutions represented typical variations of the stress and dyadic coping patterns of the couples, and could be arranged in a three- (lower, medium, and higher stress) by-two (positively vs. negatively balanced dyadic coping pattern) array. Further analyses indicated the general trend that couples with lower (vs. higher) stress together with more positively (vs. negatively) balanced dyadic coping may have experienced better functioning in projects (more positive emotions and higher sense of community) and higher relationship satisfaction. Results confirm that the partners’ pursuit of their personal projects is embedded in their relationship, and their functioning in these projects may partly depend on dyadic coping with the stress that arises during the accomplishment of the project. By using a pattern-oriented approach to dyadic data, we were able to distill stress and coping patterns that capture the specific types of couples’ relationships and indicate the non-linear and multidimensional nature of stress and dyadic coping processes

Cytoprotective dibenzoylmethane derivatives protect cells from oxidative stress-induced necrotic cell death

Screening of a small in-house library of 1863 compounds identified 29 compounds that protected Jurkat cells from hydrogen peroxide-induced cytotoxicity. From the cytoprotective compounds eleven proved to possess antioxidant activity (ABTS radical scavenger effect) and two were found to inhibit poly(ADP-ribosyl)ation (PARylation), a cytotoxic pathway operating in severely injured cells. Four cytoprotective dibenzoylmethane (DBM) derivatives were investigated in more detail as they did not scavenge hydrogen peroxide nor did they inhibit PARylation. These compounds protected cells from necrotic cell death while caspase activation, a parameter of apoptotic cell death was not affected. Hydrogen peroxide activated extracellular signal regulated kinase (ERK1/2) and p38 MAP kinases but not c-Jun N-terminal kinase (JNK). The cytoprotective DBMs suppressed the activation of Erk1/2 but not that of p38. Cytoprotection was confirmed in another cell type (A549 lung epithelial cells), indicating that the cytoprotective effect is not cell type specific. In conclusion we identified DBM analogs as a novel class of cytoprotective compounds inhibiting ERK1/2 kinase and protecting from necrotic cell death by a mechanism independent of poly(ADP-ribose) polymerase inhibition