Mechanisms and Evolution of Oxidative Sulfur Metabolism in Green Sulfur Bacteria

Green sulfur bacteria (GSB) constitute a closely related group of photoautotrophic and thiotrophic bacteria with limited phenotypic variation. They typically oxidize sulfide and thiosulfate to sulfate with sulfur globules as an intermediate. Based on genome sequence information from 15 strains, the distribution and phylogeny of enzymes involved in their oxidative sulfur metabolism was investigated. At least one homolog of sulfide:quinone oxidoreductase (SQR) is present in all strains. In all sulfur-oxidizing GSB strains except the earliest diverging Chloroherpeton thalassium, the sulfide oxidation product is further oxidized to sulfite by the dissimilatory sulfite reductase (DSR) system. This system consists of components horizontally acquired partly from sulfide-oxidizing and partly from sulfate-reducing bacteria. Depending on the strain, the sulfite is probably oxidized to sulfate by one of two different mechanisms that have different evolutionary origins: adenosine-5′-phosphosulfate reductase or polysulfide reductase-like complex 3. Thiosulfate utilization by the SOX system in GSB has apparently been acquired horizontally from Proteobacteria. SoxCD does not occur in GSB, and its function in sulfate formation in other bacteria has been replaced by the DSR system in GSB. Sequence analyses suggested that the conserved soxJXYZAKBW gene cluster was horizontally acquired by Chlorobium phaeovibrioides DSM 265 from the Chlorobaculum lineage and that this acquisition was mediated by a mobile genetic element. Thus, the last common ancestor of currently known GSB was probably photoautotrophic, hydrogenotrophic, and contained SQR but not DSR or SOX. In addition, the predominance of the Chlorobium–Chlorobaculum–Prosthecochloris lineage among cultured GSB could be due to the horizontally acquired DSR and SOX systems. Finally, based upon structural, biochemical, and phylogenetic analyses, a uniform nomenclature is suggested for sqr genes in prokaryotes

Dominance of a clonal green sulfur bacterial population in a stratified lake

For many years, the chemocline of the meromictic Lake Cadagno, Switzerland, was dominated by purple sulfur bacteria. However, following a major community shift in recent years, green sulfur bacteria (GSB) have come to dominate. We investigated this community by performing microbial diversity surveys using FISH cell counting and population multilocus sequence typing [clone library sequence analysis of the small subunit (SSU) rRNA locus and two loci involved in photosynthesis in GSB: fmoA and csmCA]. All bacterial populations clearly stratified according to water column chemistry. The GSB population peaked in the chemocline (c. 8 × 106 GSB cells mL−1) and constituted about 50% of all cells in the anoxic zones of the water column. At least 99.5% of these GSB cells had SSU rRNA, fmoA, and csmCA sequences essentially identical to that of the previously isolated and genome-sequenced GSB Chlorobium clathratiforme strain BU-1 (DSM 5477). This ribotype was not detected in Lake Cadagno before the bloom of GSB. These observations suggest that the C. clathratiforme population that has stabilized in Lake Cadagno is clonal. We speculate that such a clonal bloom could be caused by environmental disturbance, mutational adaptation, or invasio

A novel approach for navigational guidance of ships using onboard monitoring systems

A novel approach and conceptual ideas are outlined for risk-based navigational guidance of ships using decision support systems in combination with onboard, in-service monitoring systems. The guidance has as the main objective to advise on speed and/or course changes; in particular with focus on ship operations in rough weather. It is strived for to make use of a probabilistic framework considering the mathematical procedures that the guidance relies upon. The paper presents a novel concept which has the possibility to increase the reliability of the provided guidance, although information about on-site sea state parameters not necessarily is in complete agreement with the unknown and true wave parameters, nor may the hydrodynamical models of the vessel give a perfect quantitatively description of the vessel in waves. The paper includes an analysis of full-scale motion measurements and the proposed concept for navigational guidance gives promising results

Greenland Geothermal Heat Flow Database and Map (Version 1)

We compile and analyze all available geothermal heat flow measurements collected in and around Greenland into a new database of 419 sites and generate an accompanying spatial map. This database includes 290 sites previously reported by the International Heat Flow Commission (IHFC), for which we now standardize measurement and metadata quality. This database also includes 129 new sites, which have not been previously reported by the IHFC. These new sites consist of 88 offshore measurements and 41 onshore measurements, of which 24 are subglacial. We employ machine learning to synthesize these in situ measurements into a gridded geothermal heat flow model that is consistent across both continental and marine areas in and around Greenland. This model has a native horizontal resolution of 55ĝ€¯km. In comparison to five existing Greenland geothermal heat flow models, our model has the lowest mean geothermal heat flow for Greenland onshore areas. Our modeled heat flow in central North Greenland is highly sensitive to whether the NGRIP (North GReenland Ice core Project) elevated heat flow anomaly is included in the training dataset. Our model's most distinctive spatial feature is pronounced low geothermal heat flow (<ĝ€¯40ĝ€¯mWĝ€¯m-2) across the North Atlantic Craton of southern Greenland. Crucially, our model does not show an area of elevated heat flow that might be interpreted as remnant from the Icelandic plume track. Finally, we discuss the substantial influence of paleoclimatic and other corrections on geothermal heat flow measurements in Greenland. The in situ measurement database and gridded heat flow model, as well as other supporting materials, are freely available from the GEUS Dataverse (10.22008/FK2/F9P03L; Colgan and Wansing, 2021).publishedVersionPeer reviewe

The north-east Baffin Bay region, offshore Greenland – a new frontier petroleum exploration region

In recent years the Arctic has come into focus for hydrocarbon exploration, and areas offshore both West and East Greenland have been evaluated as promising frontier hydrocarbon provinces. Seven hydrocarbon exploration and exploitation licenses were awarded in 2007–2008 offshore the Disko–Nuussuaq region (Fig. 1), and two more have been awarded in the open-door region offshore south-western Greenland. In 2007, an extensive amount of new seismic and aeromagnetic data was acquired by the TGS-NOPEC Geophysical Company in the north-eastern Baffin Bay region. Geophysical mapping has been initiated by the Geological Survey of Denmark and Greenland (GEUS) in the Melville Bugt region offshore North-West Greenland (Fig. 1) with the purpose of evaluating the hydrocarbon prospectivity. Initial interpretation of seismic and gravity data suggests the presence of deep sedimentary basins separated by structural highs. Geological information on source rock, reservoir rock and seal intervals from surrounding regions suggest that the Melville Bugt region is likely to have a significant petroleum potential. The study is based on public domain magnetic and gravity data, and all proprietary and public 2-D seismic data (Fig. 1) acquired before 2003. Seismic horizons from the ‘seismic basement’ to ‘base Quaternary’ are being interpreted regionally. Based on the seismic interpretation, a structural element map, depth-structure maps and isopach maps will be produced in order to assess the prospectivity of the Melville Bugt region