Understanding the relationship between human rights abuse, state dysfunction and postcolonial sovereignty in Africa

This article explores the interrelationship between the phenomena of state dysfunction, human rights abuse and postcolonial states in the African context.  The incidence and extent of state dysfunction and human rights abuse are evaluated empirically, which reveals that dysfunctional states in Africa are generally guilty of neglecting human rights.  I attempting to understand this apparent correlation, the politico-juridical construct of negative sovereignty, as formulated by Robert Jackson, is analysed with specific reference to postcolonial African states.  The analysis suggests, paradoxically, dysfunctional states may utilise the same normative precepts that served as justifications for decolonisation (such as self-determination and non-intervention) to obfuscate or obstruct the scrutiny of human rights domestically. From these insights it is posited that functional states, both in institutional and political terms, may serve as the most effective bulwark of human rights in Africa, and that the phenomenon of state dysfunction as it relates to domestic human rights violations warrants more consideration.https://doi.org/10.19108/KOERS.82.1.230

Die universiteitswese in Suid-Afrika: ’n Bestekopname van huidige tendense en die vooruitsig vir Afrikaans

Die doel van hierdie artikel is die identifisering en analitiese omskrywing van die mees prominente politieke tendense wat tans die Suid-Afrikaanse universiteitswese beïnvloed, met spesifieke verwysing na Afrikaanse universiteite en hoër onderwys. Die bydrae neem as vertrekpunt die teoretiese beginsel van transformasie, en die spesifieke en eiesoortige ideologiese toepassing van dié konstruk in Suid-Afrika sedert 1994.  Die ingrypende invloed van hierdie ideologiese transformasiebeskouing op openbare instellings in Suid-Afrika bespreek, insluitend die institusionele en sosio-politiese impak op universiteite.  Teen hierdie teoretiese agtergrond word sleutelkwessies bespreek wat betrekking het op die Suid-Afrikaanse universiteitswese en die plek en rol van Afrikaans in besonder, insluitend in ?n bestekopname van die posisie van Afrikaans in die hoër onderwyssektor; ?n evaluasie van die transformasie van die hoër onderwyssektor; die rasionele argumente en gronde vir die behoud en bevordering van Afrikaans in die sektor; en die reaksie vanuit die Afrikaanssprekende gemeenskap teenoor die marginalisering van die taal by universiteite ?n Evaluerende toekomsperspektief word gebied waarin die behoud van Afrikaans oorweeg word, met inbegrip van aktivisme, geregtelike strategieë en die uitbouing van Afrikaans deur middel van ?n privaat hoër onderwysinstelling. https://doi.org/10.19108/KOERS.81.3.225

Hypertriglyceridaemia and the risk of pancreatitis six months post lopinavir/ritonavir initiation

Background: Hypertriglyceridaemia (HTG) is an important risk factor for pancreatitis and cardiovascular disease (CVD), depending on severity. Hypertriglyceridaemia is common in human immunodeficiency virus (HIV) infection and is also a common complication of lopinavir/ritonavir (LPV/r).Objectives: To evaluate the risk of pancreatitis associated with HTG in patients six months post initiation of LPV/r-based therapy in a regional public hospital.Methods: Triglyceride (TG), serum amylase (s-amylase) and CD4+ count values were retrospectively investigated six months post LPV/r-based initiation. Age, gender, previous antiretroviral regimen and period since HIV diagnosis were also recorded.Results: The final sample consisted of 194 patients, 50 males and 144 females; mean (± standard deviation [s.d.]) age was 39.52 (± 9.98) years, and the mean (± s.d.) period since HIV diagnosis was 91.32 (± 25.18) months. Normal TG levels (< 1.70 mmol/L) were detected in only 55% of patients and the rest presented with some degree of HTG. The mean (± s.d.) TG for the entire sample was elevated at 1.94 (± 1.30) mmol/L with the mean (± s.d.) of the males at 2.36 (± 1.74) – statistically higher compared to the females at 1.79 (± 1.08) mmol/L (p = 0.034). No cases of pancreatitis were recorded and the time since HIV diagnosis did not indicate any statistically significant differences in the means of the TG, serum amylase or CD4 count values.Conclusion: Triglyceride levels were not substantially elevated to induce pancreatitis at six months post initiation of LPV/r, but were elevated above the accepted upper normal limit of 1.70 mmol/L which may have implications for cardiovascular risk

Towards a typology of government interventionism in municipalities

Although significant progress has been made since democratisation in 1994, much still needs to be done before all local, district and metropolitan municipalities in South Africa are fully functional, sustainable, and developmental. In response to general municipal dysfunctionalism with inadequate service delivery levels and rising levels of public protest, the South African Government has a statutory and moral obligation to intervene in the affairs of municipalities. The nature, scope, and intensions of such interventions are, however, not always clear. It is evident though that Government increasingly views interventionism as a viable approach. Embracing such an interventionist paradigm in government requires scholars to more closely scrutinise municipal interventions, not as loose-standing and isolated occurrences, but as part of an emergent strategy in South African governance. The purpose of this article is to make a contextual and conceptual contribution to the analysis of interventionism by developing a theoretical construct in the form of a typology. This typology could stimulate further scholarly perspectives into the phenomenon of government interventionism in South African municipalities

COMT val158met Polymorphism and Neural Pain Processing

A functional polymorphism (val158met) of the gene coding for Catechol-O-methyltransferase (COM) has been demonstrated to be related to processing of emotional stimuli. Also, this polymorphism has been found to be associated with pain regulation in healthy subjects. Therefore, we investigated a possible influence of this polymorphism on pain processing in healthy persons as well as in subjects with markedly reduced pain sensitivity in the context of Borderline Personality Disorder (BPD). Fifty females (25 patients with BPD and 25 healthy control participants) were included in this study. Genotype had a significant – though moderate - effect on pain sensitivity, but only in healthies. The number of val alleles was correlated with the BOLD response in several pain-processing brain regions, including dorsolateral prefrontal cortex, posterior parietal cortex, lateral globus pallidus, anterior and posterior insula. Within the subgroup of healthy participants, the number of val alleles was positively correlated with the BOLD response in posterior parietal, posterior cingulate, and dorsolateral prefrontal cortex. BPD patients revealed a positive correlation between the number of val alleles and BOLD signal in anterior and posterior insula. Thus, our data show that the val158met polymorphism in the COMT gene contributes significantly to inter-individual differences in neural pain processing: in healthy people, this polymorphism was more related to cognitive aspects of pain processing, whereas BPD patients with reduced pain sensitivity showed an association with activity in brain regions related to affective pain processing

Oxytocin neurones: intrinsic mechanisms governing the regularity of spiking activity

Oxytocin neurones of the rat supraoptic nucleus are osmoresponsive and, with all other things being equal, they fire at a mean rate that is proportional to the plasma sodium concentration. However, individual spike times are governed by highly stochastic events, namely the random occurrences of excitatory synaptic inputs, the probability of which is increased by increasing extracellular osmotic pressure. Accordingly, interspike intervals (ISIs) are very irregular. In the present study, we show, by statistical analyses of firing patterns in oxytocin neurones, that the mean firing rate as measured in bins of a few seconds is more regular than expected from the variability of ISIs. This is consistent with an intrinsic activity‐dependent negative‐feedback mechanism. To test this, we compared observed neuronal firing patterns with firing patterns generated by a leaky integrate‐and‐fire model neurone, modified to exhibit activity‐dependent mechanisms known to be present in oxytocin neurones. The presence of a prolonged afterhyperpolarisation (AHP) was critical for the ability to mimic the observed regularisation of mean firing rate, although we also had to add a depolarising afterpotential (DAP; sometimes called an afterdepolarisation) to the model to match the observed ISI distributions. We tested this model by comparing its behaviour with the behaviour of oxytocin neurones exposed to apamin, a blocker of the medium AHP. Good fits indicate that the medium AHP actively contributes to the firing patterns of oxytocin neurones during non‐bursting activity, and that oxytocin neurones generally express a DAP, even though this is usually masked by superposition of a larger AHP

Dehydration-induced modulation of kappa-opioid inhibition of vasopressin neurone activity

Dehydration increases vasopressin (antidiuretic hormone) secretion from the posterior pituitary gland to reduce water loss in the urine. Vasopressin secretion is determined by action potential firing in vasopressin neurones, which can exhibit continuous, phasic (alternating periods of activity and silence), or irregular activity. Autocrine κ-opioid inhibition contributes to the generation of activity patterning of vasopressin neurones under basal conditions and so we used in vivo extracellular single unit recording to test the hypothesis that changes in autocrine κ-opioid inhibition drive changes in activity patterning of vasopressin neurones during dehydration. Dehydration increased the firing rate of rat vasopressin neurones displaying continuous activity (from 7.1 ± 0.5 to 9.0 ± 0.6 spikes s(−1)) and phasic activity (from 4.2 ± 0.7 to 7.8 ± 0.9 spikes s(−1)), but not those displaying irregular activity. The dehydration-induced increase in phasic activity was via an increase in intraburst firing rate. The selective κ-opioid receptor antagonist nor-binaltorphimine increased the firing rate of phasic neurones in non-dehydrated rats (from 3.4 ± 0.8 to 5.3 ± 0.6 spikes s(−1)) and dehydrated rats (from 6.4 ± 0.5 to 9.1 ± 1.2 spikes s(−1)), indicating that κ-opioid feedback inhibition of phasic bursts is maintained during dehydration. In a separate series of experiments, prodynorphin mRNA expression was increased in vasopressin neurones of hyperosmotic rats, compared to hypo-osmotic rats. Hence, it appears that dynorphin expression in vasopressin neurones undergoes dynamic changes in proportion to the required secretion of vasopressin so that, even under stimulated conditions, autocrine feedback inhibition of vasopressin neurones prevents over-excitation

Evidence for recombinant GRP78, CALR, PDIA3 and GPI as mediators of genetic instability in human CD34+ cells

Soluble factors released from irradiated human mesenchymal stromal cells (MSC) may induce genetic instability in human CD34+ cells, potentially mediating hematologic disorders. Recently, we identified four key proteins in the secretome of X-ray-irradiated MSC, among them three endoplasmic reticulum proteins, the 78 kDa glucose-related protein (GRP78), calreticulin (CALR), and protein disulfide-isomerase A3 (PDIA3), as well as the glycolytic enzyme glucose-6-phosphate isomerase (GPI). Here, we demonstrate that exposition of CD34+ cells to recombinant GRP78, CALR, PDIA3 and GPI induces substantial genetic instability. Increased numbers of γH2AX foci (p < 0.0001), centrosome anomalies (p = 0.1000) and aberrant metaphases (p = 0.0022) were detected in CD34+ cells upon incubation with these factors. Specifically, γH2AX foci were found to be induced 4–5-fold in response to any individual of the four factors, and centrosome anomalies by 3–4 fold compared to control medium, which contained none of the recombinant proteins. Aberrant metaphases, not seen in the context of control medium, were detected to a similar extent than centrosome anomalies across the four factors. Notably, the strongest effects were observed when all four factors were collectively provided. In summary, our data suggest that specific components of the secretome from irradiated MSC act as mediators of genetic instability in CD34+ cells, thereby possibly contributing to the pathogenesis of radiation-induced hematologic disorders beyond direct radiation-evoked DNA strand breaks

Contact heat evoked potentials using simultaneous EEG and fMRI and their correlation with evoked pain

BACKGROUND: The Contact Heat Evoked Potential Stimulator (CHEPS) utilises rapidly delivered heat pulses with adjustable peak temperatures to stimulate the differential warm/heat thresholds of receptors expressed by Adelta and C fibres. The resulting evoked potentials can be recorded and measured, providing a useful clinical tool for the study of thermal and nociceptive pathways. Concurrent recording of contact heat evoked potentials using electroencephalogram (EEG) and functional magnetic resonance imaging (fMRI) has not previously been reported with CHEPS. Developing simultaneous EEG and fMRI with CHEPS is highly desirable, as it provides an opportunity to exploit the high temporal resolution of EEG and the high spatial resolution of fMRI to study the reaction of the human brain to thermal and nociceptive stimuli. METHODS: In this study we have recorded evoked potentials stimulated by 51° C contact heat pulses from CHEPS using EEG, under normal conditions (baseline), and during continuous and simultaneous acquisition of fMRI images in ten healthy volunteers, during two sessions. The pain evoked by CHEPS was recorded on a Visual Analogue Scale (VAS). RESULTS: Analysis of EEG data revealed that the latencies and amplitudes of evoked potentials recorded during continuous fMRI did not differ significantly from baseline recordings. fMRI results were consistent with previous thermal pain studies, and showed Blood Oxygen Level Dependent (BOLD) changes in the insula, post-central gyrus, supplementary motor area (SMA), middle cingulate cortex and pre-central gyrus. There was a significant positive correlation between the evoked potential amplitude (EEG) and the psychophysical perception of pain on the VAS. CONCLUSION: The results of this study demonstrate the feasibility of recording contact heat evoked potentials with EEG during continuous and simultaneous fMRI. The combined use of the two methods can lead to identification of distinct patterns of brain activity indicative of pain and pro-nociceptive sensitisation in healthy subjects and chronic pain patients. Further studies are required for the technique to progress as a useful tool in clinical trials of novel analgesics