D6.1 Market analysis and technology database report

Within the EDEN-ISS project, a lot of technologies were implemented into the Future Expoloration Greenhouse (FEG) for the analogue mission on Antarctica. Most were existing technologies that had been developed within previous “space related” projects and some were derived from existing hightech greenhouse production technology. This document analyses the potential for spin-offs to other applications, particularly of the technologies that were either new or modifications of existing technologies, that is: the E-nose for the microbial detection; the water-cooled LED luminaries for plant lighting; the online, continuous control of the spectrum of the luminaries and the plant health monitoring system. Whereas the potential for application of the modified E-nose is particularly in hospitals and related places, the potential for the other three systems is particularly in high-tech, fresh vegetable production, such as high-tech greenhouses or Vertical Farms. Indeed, given the size of such markets, the potential for each system is certainly high. This document also gives a preview of the improvements/adaptations of each system, which would improve the penetration in the potential market

Introducing EDEN ISS - A European project on advancing plant cultivation technologies and operations

Plant cultivation in large-scale closed environments is challenging and several key technologies necessary for space-based plant production are not yet space-qualified or remain in early stages of development. The EDEN ISS project foresees development and demonstration of higher plant cultivation technologies, suitable for future deployment on the International Space Station and from a long-term perspective, within Moon and Mars habitats. The EDEN ISS consortium will design and test essential plant cultivation technologies using an International Standard Payload Rack form factor cultivation system for potential testing on-board the International Space Station. Furthermore, a Future Exploration Greenhouse will be designed with respect to future planetary bio-regenerative life support system deployments. The technologies will be tested in a laboratory environment as well as at the highly-isolated German Antarctic Neumayer Station III. A small and mobile container-sized test facility will be built in order to provide realistic mass flow relationships. In addition to technology development and validation, food safety and plant handling procedures will be developed. This paper describes the goals and objectives of EDEN ISS and the different project phases and milestones. Furthermore, the project consortium will be introduced and the role of each partner within the project is explained

The Relationship of Metabolic Syndrome with Stress, Coronary Heart Disease and Pulmonary Function - An Occupational Cohort-Based Study

<div><p>Background and Aims</p><p>Higher levels of stress impact the prevalence of metabolic syndrome (MetS) and coronary heart disease. The association between MetS, impaired pulmonary function and low level of physical activity is still pending assessment in the subjects exposed to stress. The study aimed to examine whether higher levels of stress might be related to MetS and the plaque presence, as well as whether MetS might affect pulmonary function.</p><p>Design and Methods</p><p>The study embraced 235 police officers (mean age 40.97 years) from the south of Poland. The anthropometrics and biochemical variables were measured; MetS was diagnosed using the International Diabetes Federation criteria. Computed tomography coronary angiography of coronary arteries, exercise ECG, measurements of brachial flow-mediated dilation, and carotid artery intima-media thickness were completed. In order to measure the self-perception of stress, 10-item Perceived Stress Scale (PSS-10) was applied. Pulmonary function and physical activity levels were also addressed. Multivariate logistic regression analyses were applied to determine the relationships between: 1/ incidence of coronary plaque and MetS per se, MetS components and the number of classical cardiovascular risk factors, 2/ perceived stress and MetS, 3/ MetS and pulmonary function parameters.</p><p>Results</p><p>Coronary artery atherosclerosis was less associated with MetS (OR = 2.62, 95%CI 1.24–5.52; p = 0.011) than with a co-existence of classical cardiovascular risk factors (OR = 5.67, 95% CI 1.07–29.85, p = 0.03; for 3 risk factors and OR = 9.05; 95% CI 1.24–66.23, p = 0.02; for 6 risk factors, respectively). Perceived stress increased MetS prevalence (OR = 1.07, 95% CI 1.03–1.13; p = 0.03), and impacted coronary plaque prevalence (OR = 1.05, 95% CI 1.001–1.10; p = 0.04). Leisure-time physical activity reduced the chances of developing MetS (OR = 0.98 95% CI 0.96–0.99; p = 0.02). MetS subjects had significantly lower values of certain pulmonary function parameters.</p><p>Conclusions</p><p>Exposure to job-specific stress among police officers increased the prevalence of MetS and impacted coronary plaque presence. MetS subjects had worse pulmonary function parameters. Early-stage, comprehensive therapeutic intervention may reduce overall risk of cardiovascular events and prevent pulmonary function impairment in this specific occupational population.</p></div

Th2-type cytokine-induced mucus metaplasia decreases susceptibility of human bronchial epithelium to rhinovirus infection

Human rhinoviruses (RVs) are a major cause of exacerbations in asthma and other chronic airway diseases. A characteristic feature of asthmatic epithelium is goblet cell metaplasia and mucus hypersecretion. Bronchial epithelium is also an important source of lipid mediators, including pro- and antiinflammatory eicosanoids. By using air-liquid interface cultures of airway epithelium from patients with asthma and nonasthmatic control subjects, we compared RV16 replication-induced changes in mRNA expression of asthma candidate genes and eicosanoid production in the epithelium with or without IL-13-induced mucus metaplasia. Mucus metaplastic epithelium was characterized by a 20-fold less effective replication of RV16 and blunted changes in gene expression; this effect was seen to the same extent in patients with asthma and control subjects. We identified ciliary cells as the main target for RV16 by immunofluorescence imaging and demonstrated that the numbers of ciliary cells decreased in RV16-infected epithelium. RV16 infection of mucociliary epithelium resulted in overexpression of genes associated with bronchial remodeling (e.g., MUC5AC, FGF2, and HBEGF), induction of cyclooxygenase-2, and increased secretion of prostaglandins. These responses were similar in both studied groups. These data indicate that structural changes associated with mucus metaplasia renders airway epithelium less susceptible to RV infection. Thus, exacerbations of the lung disease caused by RV may result from severe impairment in mucociliary clearance or activation of immune defense rather than from preferential infection of mucus metaplastic epithelium. Repeated rhinoviral infections of compromised epithelium may contribute to the remodeling of the airways

Use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens.

Rapid and accurate detection and identification of viruses causing respiratory tract infections is important for patient care and disease control. Despite the fact that several assays are available, identification of an etiological agent is not possible in ~30% of patients suffering from respiratory tract diseases. Therefore, the aim of the current study was to develop a diagnostic set for the detection of respiratory viruses with sensitivity as low as 1-10 copies per reaction. Evaluation of the assay using a training clinical sample set showed that viral nucleic acids were identified in ~76% of cases. To improve assay performance and facilitate the identification of novel species or emerging strains, cultures of fully differentiated human airway epithelium were used to pre-amplify infectious viruses. This additional step resulted in the detection of pathogens in all samples tested. Based on these results it can be hypothesized that the lack of an etiological agent in some clinical samples, both reported previously and observed in the present study, may result not only from the presence of unknown viral species, but also from imperfections in the detection methods used

Clinical characteristics of the study subjects.

<p>* Data are expressed as mean (SD); Framingham Risk Score, 10-year risk of developing coronary heart disease; W/H ratio, waist/hip; CHD, coronary heart disease; PCI, percutaneous coronary intervention; BMI, body mass index; ACEI, Angiotensin converting enzyme inhibitors; ARB, Angiotensin II receptor blockers; COPD, chronic obstructive pulmonary disease. Obesity was defined as BMI>30.0 kg/m² and overweight as BMI >25.0 kg/m².</p><p>Clinical characteristics of the study subjects.</p

Results of logistic regression analysis: odds ratios for coronary plaque prevalence according to MetS components adjusted for age, sex, smoking and perceived stress score.

<p>Data are expressed as ORs and corresponding 95% CIs.</p><p>OR, odd ratio; CI, confidence interval; PSS, perceived stress score.</p><p>* ≥ 94/80 cm (men/women). Hypertension was defined as blood pressure systolic/diastolic ≥130/≥85 mm Hg or treatment of previously diagnosed hypertension; hypertriglyceridemia was defined as total triglycerides ≥1.7 mmol/L (150.0 mg/dL) or specific treatment for this lipid abnormality, low HDL-cholesterol was defined when cholesterol in this lipoprotein fraction was <1.03 mmol/L (40.0 mg/dL) in men and <1.29 mmol/L (50.0 mg/dL) in women; elevated fasting blood glucose (FBG) was defined when fasting glucose ≥5.6 mmol/L (100.0 mg/dL) or previously diagnosed type 2 diabetes.</p><p>Results of logistic regression analysis: odds ratios for coronary plaque prevalence according to MetS components adjusted for age, sex, smoking and perceived stress score.</p