Influence of the Temperature and the Genotype of the HSP90AA1 Gene over Sperm Chromatin Stability in Manchega Rams

The present study addresses the effect of heat stress on males' reproduction ability. For that, we have evaluated the sperm DNA fragmentation (DFI) by SCSA of ejaculates incubated at 37°C during 0, 24 and 48 hours after its collection, as a way to mimic the temperature circumstances to which spermatozoa will be subject to in the ewe uterus. The effects of temperature and temperature-humidity index (THI) from day 60 prior collection to the date of semen collection on DFI were examined. To better understand the causes determining the sensitivity of spermatozoa to heat, this study was conducted in 60 males with alternative genotypes for the SNP G/C−660 of the HSP90AA1 promoter, which encode for the Hsp90α protein. The Hsp90α protein predominates in the brain and testis, and its role in spermatogenesis has been described in several species. Ridge regression analyses showed that days 29 to 35 and 7 to 14 before sperm collection (bsc) were the most critical regarding the effect of heat stress over DFI values. Mixed model analyses revealed that DFI increases over a threshold of 30°C for maximum temperature and 22 for THI at days 29 to 35 and 7 to 14 bsc only in animals carrying the GG−660 genotype. The period 29–35 bsc coincide with the meiosis I process for which the effect of the Hsp90α has been described in mice. The period 7–14 bsc may correspond with later stages of the meiosis II and early stages of epididymal maturation in which the replacement of histones by protamines occurs. Because of GG−660 genotype has been associated to lower levels of HSP90AA1 expression, suboptimal amounts of HSP90AA1 mRNA in GG−660 animals under heat stress conditions make spermatozoa DNA more susceptible to be fragmented. Thus, selecting against the GG−660 genotype could decrease the DNA fragmentation and spermatozoa thermal susceptibility in the heat season, and its putative subsequent fertility gainsPublishe

Influence of Sex and Maximum Strength on Reactive Strength Index-Modified

Reactive strength index-modified (RSImod) is a measure of lower body explosiveness calculated by dividing jump height by time to takeoff. RSImod is different between stronger and weaker athletes and between males and females. The purpose of this study was to evaluate differences in RSImod between males and females while controlling for maximal strength and lower body explosiveness. Forty-three female and fifty-eight male Division-I athletes performed countermovement jumps on a force plate during unloaded (0kg) and loaded (20kg) conditions. We used an ANCOVA to test whether RSImod is different between sexes conditioning on relative maximum strength (PFa) and average RFD 0-200ms (RFD200) measured during the isometric midthigh pull (IMTP). Differences of 0.087 (95% CI: 0.040 - 0.134; p = 0.0005) and 0.075 (95% CI: 0.040 - 0.109, p \u3c 0.0001) were observed for RSImod between sexes in unloaded and loaded conditions, respectively. A male with PFa of 186 (grand mean of the sample) and RFD200 of 6602 N/s (grand mean of the sample) is predicted to have 28% greater RSImod than a female of similar PFa and RFD200. Maximum strength development should be a primary aim of training in female athletes, in addition to other trainable factors, such as stiffness and RFD