50,566 research outputs found
Human sperm accumulation near surfaces: a simulation study
A hybrid boundary integral/slender body algorithm for modelling flagellar cell motility is presented. The algorithm uses the boundary element method to represent the âwedge-shapedâ head of the human sperm cell and a slender body theory representation of the flagellum. The head morphology is specified carefully due to its significant effect on the force and torque balance and hence movement of the free-swimming cell. The technique is used to investigate the mechanisms for the accumulation of human spermatozoa near surfaces. Sperm swimming in an infinite fluid, and near a plane boundary, with prescribed planar and three-dimensional flagellar waveforms are simulated. Both planar and âelliptical helicoidâ beating cells are predicted to accumulate at distances of approximately 8.5â22 ÎŒm from surfaces, for flagellar beating with angular wavenumber of 3Ï to 4Ï. Planar beating cells with wavenumber of approximately 2.4Ï or greater are predicted to accumulate at a finite distance, while cells with wavenumber of approximately 2Ï or less are predicted to escape from the surface, likely due to the breakdown of the stable swimming configuration. In the stable swimming trajectory the cell has a small angle of inclination away from the surface, no greater than approximately 0.5°. The trapping effect need not depend on specialized non-planar components of the flagellar beat but rather is a consequence of force and torque balance and the physical effect of the image systems in a no-slip plane boundary. The effect is relatively weak, so that a cell initially one body length from the surface and inclined at an angle of 4°â6° towards the surface will not be trapped but will rather be deflected from the surface. Cells performing rolling motility, where the flagellum sweeps out a âconical envelopeâ, are predicted to align with the surface provided that they approach with sufficiently steep angle. However simulation of cells swimming against a surface in such a configuration is not possible in the present framework. Simulated human sperm cells performing a planar beat with inclination between the beat plane and the plane-of-flattening of the head were not predicted to glide along surfaces, as has been observed in mouse sperm. Instead, cells initially with the head approximately 1.5â3 ÎŒm from the surface were predicted to turn away and escape. The simulation model was also used to examine rolling motility due to elliptical helicoid flagellar beating. The head was found to rotate by approximately 240° over one beat cycle and due to the time-varying torques associated with the flagellar beat was found to exhibit âloopingâ as has been observed in cells swimming against coverslips
Fluid-Induced Propulsion of Rigid Particles in Wormlike Micellar Solutions
In the absence of inertia, a reciprocal swimmer achieves no net motion in a
viscous Newtonian fluid. Here, we investigate the ability of a reciprocally
actuated particle to translate through a complex fluid that possesses a network
using tracking methods and birefringence imaging. A geometrically polar
particle, a rod with a bead on one end, is reciprocally rotated using magnetic
fields. The particle is immersed in a wormlike micellar (WLM) solution that is
known to be susceptible to the formation of shear bands and other localized
structures due to shear-induced remodeling of its microstructure. Results show
that the nonlinearities present in this WLM solution break time-reversal
symmetry under certain conditions, and enable propulsion of an artificial
"swimmer." We find three regimes dependent on the Deborah number (De): net
motion towards the bead-end of the particle at low De, net motion towards the
rod-end of the particle at intermediate De, and no appreciable propulsion at
high De. At low De, where the particle time-scale is longer then the fluid
relaxation time, we believe that propulsion is caused by an imbalance in the
fluid first normal stress differences between the two ends of the particle
(bead and rod). At De~1, however, we observe the emergence of a region of
network anisotropy near the rod using birefringence imaging. This anisotropy
suggests alignment of the micellar network, which is "locked in" due to the
shorter time-scale of the particle relative to the fluid
Instant restore after a media failure
Media failures usually leave database systems unavailable for several hours
until recovery is complete, especially in applications with large devices and
high transaction volume. Previous work introduced a technique called
single-pass restore, which increases restore bandwidth and thus substantially
decreases time to repair. Instant restore goes further as it permits read/write
access to any data on a device undergoing restore--even data not yet
restored--by restoring individual data segments on demand. Thus, the restore
process is guided primarily by the needs of applications, and the observed mean
time to repair is effectively reduced from several hours to a few seconds.
This paper presents an implementation and evaluation of instant restore. The
technique is incrementally implemented on a system starting with the
traditional ARIES design for logging and recovery. Experiments show that the
transaction latency perceived after a media failure can be cut down to less
than a second and that the overhead imposed by the technique on normal
processing is minimal. The net effect is that a few "nines" of availability are
added to the system using simple and low-overhead software techniques
Recommended from our members
VLSI design of the tiny RISC microprocessor
This report describes the Tiny RISC microprocessor designed at UC Irvine. Tiny RISC is a 16-bit microprocessor and has a RISC-style architecture. The chip was fabricated by MOSIS [1] in a 2ÎŒm n-well CMOS technology. The processor has a cycle time of 70 ns
Thermal design of the space shuttle external tank
The shuttle external tank thermal design presents many challenges in meeting the stringent requirements established by the structures, main propulsion systems, and Orbiter elements. The selected thermal protection design had to meet these requirements, and ease of application, suitability for mass production considering low weight, cost, and high reliability. This development led to a spray-on-foam (SOFI) which covers the entire tank. The need and design for a SOFI material with a dual role of cryogenic insulation and ablator, and the development of the SOFI over SLA concept for high heating areas are discussed. Further issuses of minimum surface ice/frost, no debris, and the development of the TPS spray process considering the required quality and process control are examined
Requirements for in vivo IFN-Îł induction by live microfilariae of the parasitic nematode, Brugia malayi
The magnetic fields of forming solar-like stars
Magnetic fields play a crucial role at all stages of the formation of low
mass stars and planetary systems. In the final stages, in particular, they
control the kinematics of in-falling gas from circumstellar discs, and the
launching and collimation of spectacular outflows. The magnetic coupling with
the disc is thought to influence the rotational evolution of the star, while
magnetised stellar winds control the braking of more evolved stars and may
influence the migration of planets. Magnetic reconnection events trigger
energetic flares which irradiate circumstellar discs with high energy particles
that influence the disc chemistry and set the initial conditions for planet
formation. However, it is only in the past few years that the current
generation of optical spectropolarimeters have allowed the magnetic fields of
forming solar-like stars to be probed in unprecedented detail. In order to do
justice to the recent extensive observational programs new theoretical models
are being developed that incorporate magnetic fields with an observed degree of
complexity. In this review we draw together disparate results from the
classical electromagnetism, molecular physics/chemistry, and the geophysics
literature, and demonstrate how they can be adapted to construct models of the
large scale magnetospheres of stars and planets. We conclude by examining how
the incorporation of multipolar magnetic fields into new theoretical models
will drive future progress in the field through the elucidation of several
observational conundrums.Comment: 55 pages, review article accepted for publication in Reports on
Progress in Physics. Astro-ph version includes additional appendice
Functional specialization of the yeast Rho1 GTP exchange factors
Rho GTPases are regulated in complex spatiotemporal patterns that may be dependent, in part at least, on the multiplicity of their GTP exchange factors (GEFs). Here, we examine the extent of and basis for functional specialization of the Rom2 and Tus1 GEFs that activate the yeast Rho1 GTPase, the ortholog of mammalian RhoA. First, we find that these GEFs selectively activate different Rho1-effector branches. Second, the synthetic genetic networks around ROM2 and TUS1 confirm very different global in vivo roles for these GEFs. Third, the GEFs are not functionally interchangeable: Tus1 cannot replace the essential role of Rom2, even when overexpressed. Fourth, we find that Rom2 and Tus1 localize differently: Rom2 to the growing bud surface and to the bud neck at cytokinesis; Tus1 only to the bud neck but in a distinct pattern. Finally, we find that these GEFs are dependent on different protein co-factors: Rom2 function and localization is largely dependent on Ack1, a SEL1 domain containing protein; Tus1 function and localization is largely dependent on the Tus1-interacting protein Ypl066w (which we name Rgl1). We have revealed a surprising level of diversity among the Rho1 GEFs that contributes another level of complexity to the spatiotemporal control of Rho1
Modelling bacterial behaviour close to a no-slip plane boundary: the influence of bacterial geometry
We describe a boundary-element method used to model the hydrodynamics of a bacterium propelled by a single helical flagellum. Using this model, we optimize the power efficiency of swimming with respect to cell body and flagellum geometrical parameters, and find that optima for swimming in unbounded fluid and near a no-slip plane boundary are nearly indistinguishable. We also consider the novel optimization objective of torque efficiency and find a very different optimal shape. Excluding effects such as Brownian motion and electrostatic interactions, it is demonstrated that hydrodynamic forces may trap the bacterium in a stable, circular orbit near the boundary, leading to the empirically observable surface accumulation of bacteria. Furthermore, the details and even the existence of this stable orbit depend on geometrical parameters of the bacterium, as described in this article. These results shed some light on the phenomenon of surface accumulation of micro-organisms and offer hydrodynamic explanations as to why some bacteria may accumulate more readily than others based on morphology
- âŠ