Addressing the challenges of computer literacy among young Haitian adults

The objective of the research study was to identify and address challenges of capacity building required to facilitate technological competency among school students, young adults and teachers in Haiti through, a two-phase research plan. The first phase utilizes a citizen centric framework for Information and Communication Technologies for Education (ICTE) capacity development that integrates stakeholder needs, prototype development, capacity building, training, and evaluation. They include a sustainable computing platform, Computer on a Stick (COS), and teacher workstation with Internet in a Box (IIAB). The ICTE solutions are installed at six different sites in the impoverished Central Hinche province. The citizen-centric approach presented in this paper may be utilized to develop ICT solution in other countries that face similar barriers of infrastructure and financial resources. The study is planned in two phases. The research outline framework provides a systematic approach to identify immediate and obvious needs of the users, gather feedback for improvement, and incorporate incremental improvements for the engineering of artifacts. In phase 1 of the study, we adapt the citizen-centric approach for developing locally relevant ICTE capacity. The ICTE artifacts developed in this phase leverages low-cost open source solutions to facilitate the acquisition of basic technology skills and computer literary. There were three different computer solutions that we created for the Haitian community based on their needs. They are described individually as follows: Internet in a Box (IIAB): is an open source project that consolidates approximately a terabyte of world’s free information in an inexpensive device that can be connected to workstations using local area network (LAN) or using wireless. IIAB provides access to essential internet based free information and operates without any internet connection, domain name services, or internet server configurations. The IIAB content includes more than five hundreds hours of instructional videos on topics such as science, math, art, history, and literature from the Khan academy, Wikipedia in 37 languages include Creole, MIT-Haiti STEM classroom initiative content, a library of 40,000 e- books from the Project Gutenberg archives (Gutenberg, 2014), detailed world map down to street level, and a repository of open source educational software. Dafturn Ofris: Due to the shortage of readily available qualified technology support professionals, the computers have to be configured to prevent intentional or unintentional damage to the operating system and software environment. To ensure the stability of the operating system and file system, the laptops are configured with Dafturn Ofris open source software prior to their deployment in the computer labs. This configuration allows student to log on and use the laptop without impacting the computer configuration and application settings. Restarting the computer simply returns the system to its original state. Computer on Stick (COS): COS is a complete computer system that boots from the USB port on a computer. The Edubuntu operating system with persistent storage capability is implemented on the COS. It is a mature computing environment that will also enable the users to store their personal files, applications, books, music and movies on the lightweight low profile USB thumb drive-based computer operating system. Internet in a Box (IIAB): is an open source project that consolidates approximately a terabyte of world’s free information in an inexpensive device that can be connected to workstations using local area network (LAN) or using wireless. IIAB provides access to essential internet based free information and operates without any internet connection, domain name services, or internet server configurations. The IIAB content includes more than five hundreds hours of instructional videos on topics such as science, math, art, history, and literature from the Khan academy, Wikipedia in 37 languages include Creole, MIT-Haiti STEM classroom initiative content, a library of 40,000 e- books from the Project Gutenberg archives (Gutenberg, 2014), detailed world map down to street level, and a repository of open source educational software. The target audience for this research is the students, teachers, young adults, and school administrators. Laptops and COS’s were built for the target children and set up in six sites in Central Hinche. Students will learn basic computer skills from the teachers after proper training. Once in Haiti, we saw that access to technology and digital information content remains a desire for a vast majority of the Haitian youth. Although ICT may serve as an enhancer of capabilities, and producer of opportunities, the country will remain stagnant unless the doors are opened for the Haitian youth to access technology and information. What we have given the children in these sites is temporary, until better training solutions and access to technology can be provided to Haitian youth.https://scholarscompass.vcu.edu/uresposters/1125/thumbnail.jp

E-learning at University of the Arts London

This report is a systematic exploration of staff relationships with e-learning. It presents a renewed evidence base from which e-learning provision and related support can be planned particularly in a rapidly changing HE terrain and an institutional context where e-learning and academic structures are emerging from large change programmes. The research is based on 25 interviews with programme directors (PD) evenly distributed across the 4 colleges, with representatives from all discipline groups, and levels of study. The interviewees provided rich insights into attitudes to, practices in and aspirations for e-learning, but in some instances, were also limited by the newness of the PD role. While some PDs had an intimate understanding of their programme areas, others, understandably, given the newness of posts, were in the process of familiarising themselves with the work of their teams

Translational regulation contributes to the elevated CO2 response in two Solanum species.

Understanding the impact of elevated CO2 (eCO2 ) in global agriculture is important given climate change projections. Breeding climate-resilient crops depends on genetic variation within naturally varying populations. The effect of genetic variation in response to eCO2 is poorly understood, especially in crop species. We describe the different ways in which Solanum lycopersicum and its wild relative S. pennellii respond to eCO2 , from cell anatomy, to the transcriptome, and metabolome. We further validate the importance of translational regulation as a potential mechanism for plants to adaptively respond to rising levels of atmospheric CO2

Inhibiting the oncogenic translation program is an effective therapeutic strategy in multiple myeloma

Published in final edited form as: Sci Transl Med. 2017 May 10; 9(389). https://doi.org/10.1126/scitranslmed.aal2668.Multiple myeloma (MM) is a frequently incurable hematological cancer in which overactivity of MYC plays a central role, notably through up-regulation of ribosome biogenesis and translation. To better understand the oncogenic program driven by MYC and investigate its potential as a therapeutic target, we screened a chemically diverse small-molecule library for anti-MM activity. The most potent hits identified were rocaglate scaffold inhibitors of translation initiation. Expression profiling of MM cells revealed reversion of the oncogenic MYC-driven transcriptional program by CMLD010509, the most promising rocaglate. Proteome-wide reversion correlated with selective depletion of short-lived proteins that are key to MM growth and survival, most notably MYC, MDM2, CCND1, MAF, and MCL-1. The efficacy of CMLD010509 in mouse models of MM confirmed the therapeutic relevance of these findings in vivo and supports the feasibility of targeting the oncogenic MYC-driven translation program in MM with rocaglates

Cost-Efficient and Easy to Perform PCR-Based Assay to Identify Met Exon 14 Skipping in Formalin-Fixed Paraffin-Embedded (FFPE) Non-Small Cell Lung Cancer (NSCLC) Samples

MET is a receptor tyrosine kinase (RTK) that plays important roles in carcinogenesis. Despite being frequently overexpressed in cancer, clinical responses to targeting this receptor have been limited. Recently novel splicing mutations involving the loss of exon 14 (called METex14 skipping) have emerged as potential biomarkers to predict for responsiveness to targeted therapies with Met inhibitors in non-small cell lung cancer (NSCLC). Currently, the diverse genomic alterations responsible for METex14 skipping pose a challenge for routine clinical diagnostic testing. In this report, we examine three different methodologies to detect METex14 and assess their potential utility for use as a diagnostic assay for both the identification of METex14 and intra-tumoural distribution in NSCLC

Altered Expression of ACOX2 In Non-Small Cell Lung Cancer

Peroxisomes are organelles that play essential roles in many metabolic processes, but also play roles in innate immunity, signal transduction, aging and cancer. One of the main functions of peroxisomes is the processing of very-long chain fatty acids into metabolites that can be directed to the mitochondria. One key family of enzymes in this process are the peroxisomal acyl-CoA oxidases (ACOX1, ACOX2 and ACOX3), the expression of which has been shown to be dysregulated in some cancers. Very little is however known about the expression of this family of oxidases in non-small cell lung cancer (NSCLC). ACOX2 has however been suggested to be elevated at the mRNA level in over 10% of NSCLC, and in the present study using both standard and bioinformatics approaches we show that expression of ACOX2 is significantly altered in NSCLC. ACOX2 mRNA expression is linked to a number of mutated genes, and associations between ACOX2 expression and tumour mutational burden and immune cell infiltration were explored. Links between ACOX2 expression and candidate therapies for oncogenic driver mutations such as KRAS were also identified. Furthermore, levels of acyl-CoA oxidases and other associated peroxisomal genes were explored to identify further links between the peroxisomal pathway and NSCLC. The results of this biomarker driven study suggest that ACOX2 may have potential clinical utility in the diagnosis, prognosis and stratification of patients into various therapeutically targetable options

An Analysis of JADE2 in Non-Small Cell Lung Cancer (NSCLC)

The JADE family comprises three members encoded by individual genes and roles for these proteins have been identified in chromatin remodeling, cell cycle progression, cell regeneration and the DNA damage response. JADE family members, and in particular JADE2 have not been studied in any great detail in cancer. Using a series of standard biological and bioinformatics approaches we investigated JADE2 expression in surgically resected non-small cell lung cancer (NSCLC) for both mRNA and protein to examine for correlations between JADE2 expression and overall survival. Additional correlations were identified using bioinformatic analyses on multiple online datasets. Our analysis demonstrates that JADE2 expression is significantly altered in NSCLC. High expression of JADE2 is associated with a better 5-year overall survival. Links between JADE2 mRNA expression and a number of mutated genes were identified, and associations between JADE2 expression and tumor mutational burden and immune cell infiltration were explored. Potential new drugs that can target JADE2 were identified. The results of this biomarker-driven study suggest that JADE2 may have potential clinical utility in the diagnosis, prognosis and stratification of patients into various therapeutically targetable options

Eosinophils Are Important for Protection, Immunoregulation and Pathology during Infection with Nematode Microfilariae

Eosinophil responses typify both allergic and parasitic helminth disease. In helminthic disease, the role of eosinophils can be both protective in immune responses and destructive in pathological responses. To investigate whether eosinophils are involved in both protection and pathology during filarial nematode infection, we explored the role of eosinophils and their granule proteins, eosinophil peroxidase (EPO) and major basic protein-1 (MBP-1), during infection with Brugia malayi microfilariae. Using eosinophil-deficient mice (PHIL), we further clarify the role of eosinophils in clearance of microfilariae during primary, but not challenge infection in vivo. Deletion of EPO or MBP-1 alone was insufficient to abrogate parasite clearance suggesting that either these molecules are redundant or eosinophils act indirectly in parasite clearance via augmentation of other protective responses. Absence of eosinophils increased mast cell recruitment, but not other cell types, into the broncho-alveolar lavage fluid during challenge infection. In addition absence of eosinophils or EPO alone, augmented parasite-induced IgE responses, as measured by ELISA, demonstrating that eosinophils are involved in regulation of IgE. Whole body plethysmography indicated that nematode-induced changes in airway physiology were reduced in challenge infection in the absence of eosinophils and also during primary infection in the absence of EPO alone. However lack of eosinophils or MBP-1 actually increased goblet cell mucus production. We did not find any major differences in cytokine responses in the absence of eosinophils, EPO or MBP-1. These results reveal that eosinophils actively participate in regulation of IgE and goblet cell mucus production via granule secretion during nematode-induced pathology and highlight their importance both as effector cells, as damage-inducing cells and as supervisory cells that shape both innate and adaptive immunity