Optimizing dual energy cone beam CT protocols for preclinical imaging and radiation research

Objective: The aim of this work was to investigate whether quantitative dual-energy CT (DECT) imaging is feasible for small animal irradiators with an integrated cone-beam CT (CBCT) system. Methods: The optimal imaging protocols were determined by analyzing different energy combinations and dose levels. The influence of beam hardening effects and the performance of a beam hardening correction (BHC) were investigated. In addition, two systems from different manufacturers were compared in terms of errors in the extracted effective atomic numbers (Z(eff)) and relative electron densities (rho(e)) for phantom inserts with known elemental compositions and relative electron densities. Results: The optimal energy combination was determined to be 50 and 90kVp. For this combination, Z(eff) and r rho(e) can be extracted with a mean error of 0.11 and 0.010, respectively, at a dose level of 60cGy. Conclusion: Quantitative DECT imaging is feasible for small animal irradiators with an integrated CBCT system. To obtain the best results, optimizing the imaging protocols is required. Well-separated X-ray spectra and a sufficient dose level should be used to minimize the error and noise for Z(eff) and rho(e). When no BHC is applied in the image reconstruction, the size of the calibration phantom should match the size of the imaged object to limit the influence of beam hardening effects. No significant differences in Z(eff) and rho(e) errors are observed between the two systems from different manufacturers. Advances in knowledge: This is the first study that investigates quantitative DECT imaging for small animal irradiators with an integrated CBCT system

Protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage

Electroporation is a technique to introduce DNA constructs into cells using electric current. Here, we present a protocol to electroporate DNA plasmids into Ciona robusta embryos at the 1-cell stage. We describe steps for setting up and conducting electroporation. We then detail procedures for collecting, fixing, and mounting embryos and counting expression. This protocol can be used to study the expression of enhancers via reporter assays, manipulating cells using genes or modified genes such as dominant negatives, and genome editing. For complete details on the use and execution of this protocol, please refer to Song, et al.1

The UQAM Mummy – The Use of Non-Destructive Imaging to Reconstruct an Ancient Osteobiography and to Document Modern Malfeasance

An Egyptian mummy and her coffin dating to the 26th Dynasty were donated to the École de Beaux Arts in Montreal in 1927. This mummy has been in the collection of the Université du Québec à Montréal since 1967. Inscriptions on the elaborate coffin identify the individual as Hetep-Bastet. In 1969, the mummy was attacked by a protester, who caused extensive damage. The mummy was scanned once over a decade ago. However, computed tomography (CT) technology has advanced a great deal since that time, and some conclusions reached were somewhat suspect (e.g. that she suffered from a large dental abscess caused by “drinking too much beer”). Thus, when Hetep-Bastet was transported to Gatineau in the fall of 2008 to be part of the “Tombs for Eternity” exhibit at the Canadian Museum of Civilization, we took the opportunity to rescan her. The specific goals of our study were: to assess the damage done by the protester in 1969 to investigate the specific details of how she was mummified as part of an ongoing study of variability in mummification practice to gather osteological and paleopathological data in order to reconstruct her osteobiography to segment the skull from the CT data in order to create a facial reconstruction to examine her coffin as part of an ongoing study of the use of CT scans to characterize different materials associated with Egyptian mummies Damag

The ROM / UWO Mummy Project: A Microcosm of Progress in Mummy Research

The beginnings of the Royal Ontario Museum can be traced back to the excavations and collections of Charles Trick Currelly, a staff member of the Egyptian Exploration Fund in the early 1900s. Currelly excavated with Sir Flinders Petrie at Abydos and with Edouard Naville at Deir el Bahari. With the assistance of Robert Mond and others, Currelly amassed a rich and diverse collection that became the basis for the ROM, which opened its doors in 1914. Part of that collection included several Egyptian mummies (Currelly 1971) . The Egyptologicalholdings at the ROM include eight mummies: one dating to the Predynastic Period, five from the Pharaonic Period, one from the Roman Period and one without context. Two of these, Nakht and Djedmaatesankh, have been well studied by Peter Lewin and associates, while three more are the subjects of the current investigation. The objectives of this poster are to review the work and accomplishments of the previous research, to describe the preliminary results of the current research project and to outline directions for future work

Prognostic factors of local control and disease free survival in centrally located non-small cell lung cancer treated with stereotactic body radiation therapy

Background: Stereotactic body radiation therapy (SBRT) results in high local control (LC) rates in patients with non-small cell lung cancer (NSCLC). For central lung tumors, risk-adapted fractionation schedules are used and underdosage to the Planned Target Volume (PTV) is often accepted to respect the dose constraints of the organs at risk in order to avoid high rates of toxicity. The purpose of this study was to analyze the effect of PTV underdosage and other possible prognostic factors on localand disease control after SBRT in patients with central lung tumors. Material and Methods: Patients with centrally located NSCLC treated with SBRT were included. The doses were converted into biologically equivalent dose using a/b-value of 10 Gy (BED10). Underdosage to the PTV was defined as the (percentage of) PTV receiving less than 100 Gy BED10; (%)PTV < 100 BED10. Potential prognostic factors for LC and Disease Free Survival (DFS) were evaluated using Cox regression analysis. Results: Two hundred and twenty patients received 12 fractions of SBRT. LC-rates were 88% at 2 years and 81% at 3 years. Twenty-seven patients developed a local recurrence. Both the PTV < 100 BED10 and %PTV < 100 BED10 were not prognostic for LC. Tumor size and forced expiratory volume in 1 second (FEV1) were independently prognostic for LC. Disease progression was reported in 75 patients with DFS-rates of 66% at 2 years and 56% at 3 years. Disea

The role for high flow nasal cannula as a respiratory support strategy in adults: a clinical practice guideline

Purpose: High flow nasal cannula (HFNC) is a relatively recent respiratory support technique which delivers high flow, heated and humidified controlled concentration of oxygen via the nasal route. Recently, its use has increased for a variety of clinical indications. To guide clinical practice, we developed evidence-based recommendations regarding use of HFNC in various clinical settings. Methods: We formed a guideline panel composed of clinicians, methodologists and experts in respiratory medicine. Using GRADE, the panel developed recommendations for four actionable questions. Results: The guideline panel made a strong recommendation for HFNC in hypoxemic respiratory failure compared to conventional oxygen therapy (COT) (moderate certainty), a conditional recommendation for HFNC following extubation (moderate certainty), no recommendation regarding HFNC in the peri-intubation period (moderate certainty), and a conditional recommendation for postoperative HFNC in high risk and/or obese patients following cardiac or thoracic surgery (moderate certainty). Conclusions: This clinical practice guideline synthesizes current best-evidence into four recommendations for HFNC use in patients with hypoxemic respiratory failure, following extubation, in the peri-intubation period, and postoperatively for bedside clinicians