A Simultaneous Solution to the ^6Li and ^7Li Big Bang Nucleosynthesis Problems from a Long-Lived Negatively-Charged Leptonic Particle

The $^6$Li abundance observed in metal poor halo stars exhibits a plateau similar to that for $^7$Li suggesting a primordial origin. However, the observed abundance of $^6$Li is a factor of $10^3$ larger and that of $^7$Li is a factor of 3 lower than the abundances predicted in the standard big bang when the baryon-to-photon ratio is fixed by WMAP. Here we show that both of these abundance anomalies can be explained by the existence of a long-lived massive, negatively-charged leptonic particle during nucleosynthesis. Such particles would capture onto the synthesized nuclei thereby reducing the reaction Coulomb barriers and opening new transfer reaction possibilities, and catalyzing a second round of big bang nucleosynthesis. This novel solution to both of the Li problems can be achieved with or without the additional effects of stellar destruction.Comment: 6 pages, 2 figures, to be published in Physical Review

Feeding dairy cattle for proper body condition score (1993)

Body condition score is a critical measure of a dairy feeding system's effectiveness. Adequate body fat reserves promote milk production, reproductive efficiency and herd longevity. Excessively fat cows or overly thin cows run much greater risks of metabolic problems, lower milk yield, poor conception rates and dystocia (difficulty calving). Failure to attain proper body condition or rapid changes in body condition score during early lactation may indicate problems in herd health or feeding management. This publication will provide guidance for feeding dairy cattle for proper body condition score.Reviewed October 1993 -- Extension website

The dose-response relationship between training load and aerobic fitness in academy rugby union players

© 2018 Human Kinetics, Inc. Purpose: To identify the dose-response relationship between measures of training load (TL) and changes in aerobic fitness in academy rugby union players. Method: Training data from 10 academy rugby union players were collected during a 6-wk in-season period. Participants completed a lactate-threshold test that was used to assess VO 2 max, velocity at VO 2 max, velocity at 2 mmol/L (lactate threshold), and velocity at 4 mmol/L (onset of lactate accumulation; vOBLA) as measures of aerobic fitness. Internal-TL measures calculated were Banister training impulse (bTRIMP), Edwards TRIMP, Lucia TRIMP, individualized TRIMP (iTRIMP), and session RPE (sRPE). External-TL measures calculated were total distance, PlayerLoad™, high-speed distance > 15 km/h, very-high-speed distance > 18 km/h, and individualized high-speed distance based on each player’s vOBLA. Results: A second-order-regression (quadratic) analysis found that bTRIMP (R 2 = .78, P = .005) explained 78% of the variance and iTRIMP (R 2 = .55, P = .063) explained 55% of the variance in changes in VO 2 max. All other HR-based internal-TL measures and sRPE explained less than 40% of variance with fitness changes. External TL explained less than 42% of variance with fitness changes. Conclusions: In rugby players, bTRIMP and iTRIMP display a curvilinear dose-response relationship with changes in maximal aerobic fitness

Stepwise pumping approach to improve free phase light hydrocarbon recovery from unconfined aquifers

A stepwise, time-varying pumping approach is developed to improve free phase oil recovery of light non-aqueous phase liquids {LNAPL) from a homogeneous, unconfined aquifer. Stepwise pumping is used to contain the floating oil plume and obtain efficient free oil recovery. The pumping approach is developed using detailed simulations, multiple linear regression and graphical plots. The approach uses ARMOS©, an areal two-dimensional multiphase flow, finite-element simulation model. Systematic simulations of f~ oil area changes to pumping rates are analyzed. Pumping rates are determined that achieve LNAPL plume containment at different times (i.e. 90, 180 and 360 days) for a planning period of 360 days. These pumping rates are used in reverse order as a stepwise (monotonically increasing) pumping strategy. This stepwise pumping strategy is analyzed further by performing additional simulations at different pumping rates for the last pumping period. The final stepwise pumping strategy is varied by factors of -25% and +30% to evaluate sensitivity in the free oil recovery process. Stepwise pumping is compared to steady pumping rates to determine the best free oil recovery strategy. Stepwise pumping is shown to improve oil recovery by increasing recovered oil volume (11%) and decreasing residual oil (15%) when compared with traditional steady pumping strategies. The best stepwise pumping strategy recovers more free oil by reducing the amount of residual oil left in the system due to pumping drawdown. This stepwise pumping approach can be used to enhance free oil recovery and provide for cost-effective design and management of LNAPL cleanup

Modeling technique for optimal recovery of immiscible light hydrocarbons as free product from contaminated aquifer

Contamination sites associated with light non-aqueous phase liquids {LNAPL) are numerous and represent difficult cleanup problems. Remediation methods for cleanup of LNAPL fluids in subsurface systems are continuously evolving with the development of various technologies for pump.-and~treat, soil venting, and in-situ bioremediation. Evaluating the effectiveness of remediation techniques as well as attempting to improve their efficiency has been a focus of many researchers, These efforts have included the development of computer simulation models to predict and analyze the fluid movement, entrapment, and mobilization of three~phase systems in porous media. The capability of computer models that not only simulate but optimize remediation processes are in great need. Simulation/optimization (S/0) models allow engineers to optimize processes and to make cost-effective design and management decisions when evaluating remediation strategies. An innovative approach is presented to optimize pumping to immobilize and recover the free product of a floating contaminant consisting of LNAPL\u27s. This determines the best pumping strategy to capture and remove the free oil product not left behind as residual oil. The approach combines detailed simulation, statistical analysis, and operations research optimization. This modeling technique derives regression equations describing system response to unit pumping stimuli. The statistical approach develops regression equations that represent free oil area within the system versus pumping. These equations are derived for multiple time steps and used in the S/0 model to determine the optimized pumping rates required to minimize free oil area and entrapped oil while maximizing free oil recovery and immobilizing the plume via a pump and oil recovery system. When compared with standard simulation techniques, this approach can save substantial computational time and improve remediation strategy design. Utilized is a modified version of US/REMAX, a linear or nonlinear simulation/optimization (S/0) model. US/REMAX can be used to analyze and optimize a variety of groundwater management problems. The modified version incorporates system responses generated externally using ARMOS plus regression analysis. ARMOS is a Z~D areal multiphase flow model. It has the capability of simulating spill, leakage, redistribution, or recovery of LNAPL materials. Application to a representative field problem illustrates the S/O model utility for problem analysis and remediation design. Potential applications of such an S/0 model are numerous providing optimized strategies for recovery of LNAPL spills

Tattoo ink nanoparticles in skin tissue and fibroblasts

YesTattooing has long been practised in various societies all around the world and is becoming increasingly common and widespread in the West. Tattoo ink suspensions unquestionably contain pigments composed of nanoparticles, i.e., particles of sub-100 nm dimensions. It is widely acknowledged that nanoparticles have higher levels of chemical activity than their larger particle equivalents. However, assessment of the toxicity of tattoo inks has been the subject of little research and ink manufacturers are not obliged to disclose the exact composition of their products. This study examines tattoo ink particles in two fundamental skin components at the nanometre level. We use atomic force microscopy and light microscopy to examine cryosections of tattooed skin, exploring the collagen fibril networks in the dermis that contain ink nanoparticles. Further, we culture fibroblasts in diluted tattoo ink to explore both the immediate impact of ink pigment on cell viability and also to observe the interaction between particles and the cells

Whole-genome sequencing for national surveillance of Shiga toxin–producing Escherichia coli O157

Background. National surveillance of gastrointestinal pathogens, such as Shiga toxin–producing Escherichia coli O157 (STEC O157), is key to rapidly identifying linked cases in the distributed food network to facilitate public health interventions. In this study, we used whole-genome sequencing (WGS) as a tool to inform national surveillance of STEC O157 in terms of identifying linked cases and clusters and guiding epidemiological investigation. Methods. We retrospectively analyzed 334 isolates randomly sampled from 1002 strains of STEC O157 received by the Gastrointestinal Bacteria Reference Unit at Public Health England, Colindale, in 2012. The genetic distance between each isolate, as estimated by WGS, was calculated and phylogenetic methods were used to place strains in an evolutionary context. Results. Estimates of linked clusters representing STEC O157 outbreaks in England and Wales increased by 2-fold when WGS was used instead of traditional typing techniques. The previously unidentified clusters were often widely geographically distributed and small in size. Phylogenetic analysis facilitated identification of temporally distinct cases sharing common exposures and delineating those that shared epidemiological and temporal links. Comparison with multi locus variable number tandem repeat analysis (MLVA) showed that although MLVA is as sensitive as WGS, WGS provides a more timely resolution to outbreak clustering. Conclusions. WGS has come of age as a molecular typing tool to inform national surveillance of STEC O157; it can be used in real time to provide the highest strain-level resolution for outbreak investigation. WGS allows linked cases to be identified with unprecedented specificity and sensitivity that will facilitate targeted and appropriate public health investigations

Management of Children With Chronic Wet Cough and Protracted Bacterial Bronchitis CHEST Guideline and Expert Panel Report

BACKGROUND: Wet or productive cough is common in children with chronic cough. We formulated recommendations based on systematic reviews related to the management of chronic wet cough in children (aged METHODS: We used the CHEST expert cough panel\u27s protocol for systematic reviews and the American College of Chest Physicians (CHEST) methodologic guidelines and GRADE framework (the Grading of Recommendations Assessment, Development and Evaluation). Data from the systematic reviews in conjunction with patients\u27 values and preferences and the clinical context were used to form recommendations. Delphi methodology was used to obtain consensus for the recommendations/suggestions made. RESULTS: Combining data from the systematic reviews, we found high-quality evidence in children aged 4 weeks\u27 duration) wet/productive cough that using appropriate antibiotics improves cough resolution, and further investigations (eg, flexible bronchoscopy, chest CT scans, immunity tests) should be undertaken when specific cough pointers (eg, digital clubbing) are present. When the wet cough does not improve following 4 weeks of antibiotic treatment, there is moderate-quality evidence that further investigations should be considered to look for an underlying disease. New recommendations include the recognition of the clinical diagnostic entity of protracted bacterial bronchitis. CONCLUSIONS: Compared with the 2006 Cough Guidelines, there is now high-quality evidence for some, but not all, aspects of the management of chronic wet cough in specialist settings. However, further studies (particularly in primary health) are required

Single passage in mouse organs enhances the survival and spread of Salmonella enterica.

Intravenous inoculation of Salmonella enterica serovar Typhimurium into mice is a prime experimental model of invasive salmonellosis. The use of wild-type isogenic tagged strains (WITS) in this system has revealed that bacteria undergo independent bottlenecks in the liver and spleen before establishing a systemic infection. We recently showed that those bacteria that survived the bottleneck exhibited enhanced growth when transferred to naive mice. In this study, we set out to disentangle the components of this in vivo adaptation by inoculating mice with WITS grown either in vitro or in vivo. We developed an original method to estimate the replication and killing rates of bacteria from experimental data, which involved solving the probability-generating function of a non-homogeneous birth-death-immigration process. This revealed a low initial mortality in bacteria obtained from a donor animal. Next, an analysis of WITS distributions in the livers and spleens of recipient animals indicated that in vivo-passaged bacteria started spreading between organs earlier than in vitro-grown bacteria. These results further our understanding of the influence of passage in a host on the fitness and virulence of Salmonella enterica and represent an advance in the power of investigation on the patterns and mechanisms of host-pathogen interactions.This work was funded by a Medical Research Council (MRC) grant (G0801161) awarded to AJG, PM and DJM. RD was supported by BBSRC grant BB/I002189/1 awarded to PM. OR is supported by a University Research Fellowship from the Royal Society.This is the final version of the article. It was first available from Royal Society Publishing via http://dx.doi.org/10.1098/rsif.2015.070