Fabrication of novel strontium-coated bioactive ceramic-glass (C2S(2P6) C2S) 3D-porous scaffold for the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells

This study fabricated novel multilayer 3D-scaffolds by coating bioactive Calcium silicate (Ca2SiO4)/glass phase (calcium ultraphosphate, Ca2P6O17)/Ca2SiO4 (C2S(2P6)C2S) 3D scaffolds with strontium (Sr) for osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs). Hence, for the first time, C2S(2P6)C2S/ Sr 3D-scaffolds were fabricated by sol-gel method and their characterization (X-Ray diffraction analysis (XRD), scanning electron microscopy (SEM) and Mercury Porosimetry), effect in MSCs proliferation (cytotoxicity and mRNA expression) and osteogenic differentiation (staining and mRNA expression) were evaluated. The porosity and SEM data showed that the porosity (31.66% for C2S(2P6)C2S and 32.14% for C2S(2P6)C2S/Sr), pore size (<300 μm) and microstructure were not altered between C2S(2P6)C2S and C2S(2P6)C2S/Sr 3D-scaffolds, respectively. MSCs proliferation rate was increased by C2S(2P6)C2S/Sr 3D-scaffold via upregulating c-Fos and TGF-β1 mRNA expression. Alizarin Red (calcium), von-kossa (calcium-phosphate) and alkaline phosphatase (ALP) staining were higher in differentiated MSCs cultured on C2S(2P6)C2S/Sr 3D scaffold than in control. The osteogenic stimulatory effect of C2S(2P6)C2S/Sr 3D scaffold could be justified by increasing osteogenic stimu- latory genes such as collagen type-I, Runx2, osteocalcin and ALP expression in differentiated MSCs. Further, SEM images proved that the C2S(2P6)C2S/Sr 3D scaffold-cultured cells had unique morphology similar to biological tissues. Accordingly, this is the first report evidencing the MSC proliferative and osteogenic stimulatory ability of strontium-coated C2S(2P6)C2S 3D-scaffold, which greatly impacts future strategic therapies in dentistry and bone regeneration

Dental Pathology Booklet

Oral pathology is the discipline of Dentistry that deals with everything related to the etiopathogenesis, pathophysiology, clinical manifestations, diagnosis and prognosis of enamel, dentine and pulp affections. In this handbook we review the most frequent pathological entities at a dental level, such as trauma, consumptive processes and cariology. Focusing our efforts to provide dental students with a useful, simple teaching tool with an eminently clinical approach.La patología dental es aquella disciplina de la odontología que se ocupa de todo lo relacionado con la etiopatogenia, fisiopatología, clínica, diagnóstico y pronóstico de las afecciones del esmalte, de la dentina y de la pulpa. En este prontuario repasamos todos todas las entidades patológicas más frecuentes a nivel dental , tales como traumatismos, procesos consuntivos y cariología etc. Centrando nuestros esfuerzos en dotar a los alumnos de odontología de una herramienta didáctica útil, sencilla y con un enfoque eminentemente clínicoOdontologí

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

© 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licenseBackground: Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide. Methods: A multimethods analysis was performed as part of the GlobalSurg 3 study—a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital. Findings: Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3·85 [95% CI 2·58–5·75]; p<0·0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63·0% vs 82·7%; OR 0·35 [0·23–0·53]; p<0·0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer. Interpretation: Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised. Funding: National Institute for Health and Care Research

Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

© 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 licenseBackground: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit