Invasion biology in non-free-living species: interactions between abiotic (climatic) and biotic (host availability) factors in geographical space in crayfish commensals (Ostracoda, Entocytheridae)

In invasion processes, both abiotic and biotic factors are considered essential, but the latter are usually disregarded when modeling the potential spread of exotic species. In the framework of set theory, interactions between biotic (B), abiotic (A), and movement-related (M) factors in the geographical space can be hypothesized with BAM diagrams and tested using ecological niche models (ENMs) to estimate A and B areas. The main aim of our survey was to evaluate the interactions between abiotic (climatic) and biotic (host availability) factors in geographical space for exotic symbionts (i.e., non-free-living species), using ENM techniques combined with a BAM framework and using exotic Entocytheridae (Ostracoda) found in Europe as model organisms. We carried out an extensive survey to evaluate the distribution of entocytherids hosted by crayfish in Europe by checking 94 European localities and 12 crayfish species. Both exotic entocytherid species found, Ankylocythere sinuosa and Uncinocythere occidentalis, were widely distributed in W Europe living on the exotic crayfish species Procambarus clarkii and Pacifastacus leniusculus, respectively. No entocytherids were observed in the remaining crayfish species. The suitable area for A. sinuosa was mainly restricted by its own limitations to minimum temperatures in W and N Europe and precipitation seasonality in circum-Mediterranean areas. Uncinocythere occidentalis was mostly restricted by host availability in circum-Mediterranean regions due to limitations of P. leniusculus to higher precipitation seasonality and maximum temperatures. The combination of ENMs with set theory allows studying the invasive biology of symbionts and provides clues about biogeographic barriers due to abiotic or biotic factors limiting the expansion of the symbiont in different regions of the invasive range. The relative importance of abiotic and biotic factors on geographical space can then be assessed and applied in conservation plans. This approach can also be implemented in other systems where the target species is closely interacting with other taxa

The Danubian cryptic invader Theodoxus fluviatilis (Gastropoda: Neritidae) in the River Rhine: a potential indicator for metal pollution?

Metal pollution poses a major threat to aquatic systems especially in anthropogenic influenced areas, in as much as metals are persistent in the environment. The freshwater snail Theodoxus fluviatilis has often been used as an indicator species for the ecological status in river monitoring. In the River Rhine, the native Northern-European form of T. fluviatilis is nowadays extinct, whilst the Danubian form is spreading along the river. The aim of our study was to investigate if the cryptic invader is affected by metal exposure present in the River Rhine and to discuss its potential as an indicator for metal pollution. Several environmental abiotic (14 water environmental variables plus five common metal concentrations in water and biofilm) and biotic parameters (biofilm mass) were measured across 23 sites along the River Rhine. Five population and six histopathological parameters were evaluated on snails collected at all 23 sites. Aqueous chromium concentration was positively correlated to the damage of male reproductive organs of T. fluviatilis, and higher ammonium concentration was correlated to a decrease in snail size and an increase in the proportion of juveniles. None of the analysed snail parameters was negatively correlated to concentrations of other metals measured, like copper and zinc. Therefore, based on the parameters evaluated, our results indicate that the Danubian form of T. fluviatilis is only restrictedly suitable as an indicator for metal pollution in the River Rhine system. Further field and laboratory investigations including other stressors are necessary to evaluate the indicator potential of the cryptic invader holistically

Hemisus marmoratus (Peters, 1854) (Anura: Hemisotidae), Fortpflanzungsstrategien eines Savannenfrosches

Volume: 45Start Page: 191End Page: 20

The Danubian cryptic invader Theodoxus fluviatilis (Gastropoda: Neritidae) in the River Rhine: a potential indicator for metal pollution?

Metal pollution poses a major threat to aquatic systems especially in anthropogenic influenced areas, in as much as metals are persistent in the environment. The freshwater snail Theodoxus fluviatilis has often been used as an indicator species for the ecological status in river monitoring. In the River Rhine, the native Northern-European form of T. fluviatilis is nowadays extinct, whilst the Danubian form is spreading along the river. The aim of our study was to investigate if the cryptic invader is affected by metal exposure present in the River Rhine and to discuss its potential as an indicator for metal pollution. Several environmental abiotic (14 water environmental variables plus five common metal concentrations in water and biofilm) and biotic parameters (biofilm mass) were measured across 23 sites along the River Rhine. Five population and six histopathological parameters were evaluated on snails collected at all 23 sites. Aqueous chromium concentration was positively correlated to the damage of male reproductive organs of T. fluviatilis, and higher ammonium concentration was correlated to a decrease in snail size and an increase in the proportion of juveniles. None of the analysed snail parameters was negatively correlated to concentrations of other metals measured, like copper and zinc. Therefore, based on the parameters evaluated, our results indicate that the Danubian form of T. fluviatilis is only restrictedly suitable as an indicator for metal pollution in the River Rhine system. Further field and laboratory investigations including other stressors are necessary to evaluate the indicator potential of the cryptic invader holistically

In Vitro Study of the Interaction of Innate Immune Cells with Liquid Silicone Rubber Coated with Zwitterionic Methyl Methacrylate and Thermoplastic Polyurethanes

The biocompatibility of medical devices, such as implants and prostheses, is strongly determined by the host’s immune response to the implanted material. Monocytes and macrophages are main actors of the so-called foreign body reaction. The innate immune system macrophages (M) can be broadly classified into the pro-inflammatory M1-type and the anti-inflammatory, pro-healing M2-type. While a transient inflammatory initial state can be helpful during an infection, persistent inflammation interferes with proper healing and subsequent regeneration. The functional orientation of the immune response, mirrored by monocyte polarization, during interaction with different biomaterials has not yet been sufficiently explored. In implant manufacturing, thermoplastic polyurethane (TPU) represents the state-of-the-art material. The constantly growing areas of application and the associated necessary adaptations make the optimization of these materials indispensable. In the present study, modified liquid silicone rubber (LSR) were compared with two of the most commonly used TPUs, in terms of monocyte adhesion and M1/M2 polarization in vitro. Human monocytes isolated from venous blood were evaluated for their ability to adhere to various biomaterials, their gene expression profile, and their cytokine release. Based on the results, the different polymers exhibit different potential to bias monocytes with respect to early pro-inflammatory cytokine production and gene transcription. Furthermore, none of our test materials showed a clear trend towards M1 or M2 polarization. However, we were able to evaluate the inflammatory potential of the materials, with the classic TPUs appearing to be the most unreactive compared to the silicone-based materials

Invasion biology in non-free-living species: interactions between abiotic (climatic) and biotic (host availability) factors in geographical space in crayfish commensals (Ostracoda, Entocytheridae)

In invasion processes, both abiotic and biotic factors are considered essential, but the latter are usually disregarded when modeling the potential spread of exotic species. In the framework of set theory, interactions between biotic (B), abiotic (A), and movement-related (M) factors in the geographical space can be hypothesized with BAM diagrams and tested using ecological niche models (ENMs) to estimate A and B areas. The main aim of our survey was to evaluate the interactions between abiotic (climatic) and biotic (host availability) factors in geographical space for exotic symbionts (i.e., non-free-living species), using ENM techniques combined with a BAM framework and using exotic Entocytheridae (Ostracoda) found in Europe as model organisms. We carried out an extensive survey to evaluate the distribution of entocytherids hosted by crayfish in Europe by checking 94 European localities and 12 crayfish species. Both exotic entocytherid species found, Ankylocythere sinuosa and Uncinocythere occidentalis, were widely distributed in W Europe living on the exotic crayfish species Procambarus clarkii and Pacifastacus leniusculus, respectively. No entocytherids were observed in the remaining crayfish species. The suitable area for A.sinuosa was mainly restricted by its own limitations to minimum temperatures in W and N Europe and precipitation seasonality in circum-Mediterranean areas. Uncinocythere occidentalis was mostly restricted by host availability in circum-Mediterranean regions due to limitations of P.leniusculus to higher precipitation seasonality and maximum temperatures. The combination of ENMs with set theory allows studying the invasive biology of symbionts and provides clues about biogeographic barriers due to abiotic or biotic factors limiting the expansion of the symbiont in different regions of the invasive range. The relative importance of abiotic and biotic factors on geographical space can then be assessed and applied in conservation plans. This approach can also be implemented in other systems where the target species is closely interacting with other taxa