Polymerase-directed synthesis of C5-ethynyl locked nucleic acids

Modified nucleic acids have considerable potential in nanobiotechnology for the development of nanomedicines and new materials. Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far and we herein for the first time report the enzymatic incorporation of LNA-U and C5-ethynyl LNA-U nucleotides into oligonucleotides. Phusion High Fidelity and KOD DNA polymerases efficiently incorporated LNA-U and C5-ethynyl LNA-U nucleotides into a DNA strand and T7 RNA polymerase successfully accepted the LNA-U nucleoside 5′-triphosphate as substrate for RNA transcripts

Dépistage immunochimique des antidépresseurs tricycliques (encore à l'ordre du jour ?)

Les antidépresseurs tricycliques restent des molécules largement prescrites et sont responsables d intoxications graves potentiellement létales. Leur dépistage rapide fait partie du bilan toxicologique d urgence de première intention. Les données des fabricants sur les méthodes immunologiques, pourtant d un usage très courant, ne sont pas satisfaisantes et encouragent à les utiliser avec prudence. Nous avons étudié la sensibilité et la spécificité du kit Emit® Tox Serum Tricyclic Antidepressants Assay fabriqué par Dade-Behring. Si la sensibilité aux antidépresseurs tricycliques vrais semble satisfaisante pour la plupart des molécules, ce n est pas le cas de la spécificité. De nombreuses molécules (phénothiazines, antihistaminiques, psychotropes, neuroleptiques ou vasodilatateurs) produisent des faux positifs. En complément d un dialogue efficace analyste-clinicien, une méthode spécifique est indispensable pour confirmer le dépistage.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Mental Health Similarities and Interdependence Between Healthy Elderly Spouses: A Systematic Review and Research Directions

Several studies report steady associations between mental health outcomes of elderly spouses. Nevertheless, the specific role of marital relationships in the non-independence of mental health status is not clear given the diversity of theoretical approaches, the various methodologies used and the lack of studies focusing on people aged 60 and over. Based on the PRISMA methodology, a systematic search of PubMed, PsycINFO, and PsycARTICLES was conducted. A total of 21 articles were included. The quality of the studies was evaluated using the National Institute for Health and Clinical Excellence tool. Additional criteria were added to ensure that the associations observed are related to the marital relationship. The main finding of this systematic review is a lack of evidence for the specific role of marital relationships. Based on the current findings, we propose methodological recommendations to develop research designs more suited to address the role of marital relationships in mental health similarities and interdependence in older couples. In particular, we recommend including covariates related to marital relationships, comparisons with dyads of pseudo-couples and a development of studies in daily life. Finally, this review underscores the importance of a dyadic approach to better understand the elderly’s mental health in clinical practice

Neuroimaging findings and follow-up in two cases of severe ethylene glycol intoxication with full recovery

International audienc