318 research outputs found
Production of positive controls for calcivirus-specific PCR using recombinant baculiovirus technology
Recent advances in our knowledge of the genetic structure of human caliciviruses (HuCVs) and small round-structured viruses (SRSVs) have led to the development of polymerase chain reaction (PCR)-based molecular tests specific for these viruses. These methods have been developed to detect a number of human pathogenic viruses in environmental samples including water, sewage and shellfish. HuCVs and SRSVs are not culturable, and no animal model is currently available. Therefore there is no convenient method of preparing viruses for study or for reagent production. One problem facing those attempting to use PCR-based methods for the detection of HuCVs and SRSVs is the lack of a suitable positive control substrate. This is particularly important when screening complex samples in which the levels of inhibitors present may significantly interfere with amplificiation. Regions within the RNA polymerase regions of two genetically distinct human caliciviruses have been amplified and used to produce recombinant baculoviruses which express RNA corresponding to the calicivirus polymerase. This RNA is being investigated as a positive control substrate for PCR testing, using current diagnostic primer sets. Recombinant baculovirus technology will enable efficient and cost-effective production of large quantities of positive control RNA with a specific known genotype. We consider the development of these systems as essential for successful screening and monitoring applications
An In-Situ Ellipsometric Study of Aqueous NH(4)OH Treatment of Silicon
In-situ ellipsometry is used to analyze silicon surfaces in an aqueous NH4 OH ambient. The ellipsometric data indicate that a surface which is optically quite similar to bare Si is obtained when the native SiO2 film is etched from a Si substrate by aqueous NH4OH. In contrast, the surface obtained by etching the native oxide in aqueous buffered HF leads to formation of a residual film on the Si substrate. Roughening of sample surfaces treated with either aqueous NH.OH or BHF is observed by microscopy. The high degree of roughness obtained following NH4OH treatment appears to influence ellipsometric measurements on samples, but this influence is not observed following BHF exposure where less roughness is noted
Search for exoplanets in M31 with pixel-lensing and the PA-99-N2 event revisited
Several exoplanets have been detected towards the Galactic bulge with the
microlensing technique. We show that exoplanets in M31 may also be detected
with the pixel-lensing method, if telescopes making high cadence observations
of an ongoing microlensing event are used. Using a Monte Carlo approach we find
that the mean mass for detectable planetary systems is about .
However, even small mass exoplanets () can cause
significant deviations, which are observable with large telescopes. We
reanalysed the POINT-AGAPE microlensing event PA-99-N2. First, we test the
robustness of the binary lens conclusion for this light curve. Second, we show
that for such long duration and bright microlensing events, the efficiency for
finding planetary-like deviations is strongly enhanced with respect to that
evaluated for all planetary detectable events.Comment: 14 pages, 8 figures. Paper presented at the "II Italian-Pakistani
Workshop on Relativistic Astrophysics, Pescara, July 8-10, 2009. To be
published in a special issue of General Relativity and Gravitation (eds. F.
De Paolis, G.F.R. Ellis, A. Qadir and R. Ruffini
Atom gratings produced by large angle atom beam splitters
An asymptotic theory of atom scattering by large amplitude periodic
potentials is developed in the Raman-Nath approximation. The atom grating
profile arising after scattering is evaluated in the Fresnel zone for
triangular, sinusoidal, magneto-optical, and bichromatic field potentials. It
is shown that, owing to the scattering in these potentials, two
\QTR{em}{groups} of momentum states are produced rather than two distinct
momentum components. The corresponding spatial density profile is calculated
and found to differ significantly from a pure sinusoid.Comment: 16 pages, 7 figure
What do young athletes implicitly understand about psychological skills?
One reason sport psychologists teach psychological skills is to enhance performance in sport; but the value of psychological skills for young athletes is questionable because of the qualitative and quantitative differences between children and adults in their understanding of abstract concepts such as mental skills. To teach these skills effectively to young athletes, sport psychologists need to appreciate what young athletes implicitly understand about such skills because maturational (e.g., cognitive, social) and environmental (e.g., coaches) factors can influence the progressive development of children and youth. In the present qualitative study, we explored young athletes’ (aged 10–15 years) understanding of four basic psychological skills: goal setting, mental imagery, self-talk, and relaxation. Young athletes (n = 118: 75 males and 43 females) completed an open-ended questionnaire to report their understanding of these four basic psychological skills. Compared with the older youth athletes, the younger youth athletes were less able to explain the meaning of each psychological skill. Goal setting and mental imagery were better understood than self-talk and relaxation. Based on these findings, sport psychologists should consider adapting interventions and psychoeducational programs to match young athletes’ age and developmental level
Indirect signals from light neutralinos in supersymmetric models without gaugino mass unification
We examine indirect signals produced by neutralino self-annihilations, in the
galactic halo or inside celestial bodies, in the frame of an effective MSSM
model without gaugino-mass unification at a grand unification scale. We compare
our theoretical predictions with current experimental data of gamma-rays and
antiprotons in space and of upgoing muons at neutrino telescopes. Results are
presented for a wide range of the neutralino mass, though our discussions are
focused on light neutralinos. We find that only the antiproton signal is
potentially able to set constraints on very low-mass neutralinos, below 20 GeV.
The gamma-ray signal, both from the galactic center and from high galactic
latitudes, requires significantly steep profiles or substantial clumpiness in
order to reach detectable levels. The up-going muon signal is largely below
experimental sensitivities for the neutrino flux coming from the Sun; for the
flux from the Earth an improvement of about one order of magnitude in
experimental sensitivities (with a low energy threshold) can make accessible
neutralino masses close to O, Si and Mg nuclei masses, for which resonant
capture is operative.Comment: 17 pages, 1 tables and 5 figures, typeset with ReVTeX4. The paper may
also be found at http://www.to.infn.it/~fornengo/papers/indirect04.ps.gz or
through http://www.astroparticle.to.infn.it/. Limit from BR(Bs--> mu+ mu-)
adde
Atomic diffraction from nanostructured optical potentials
We develop a versatile theoretical approach to the study of cold-atom
diffractive scattering from light-field gratings by combining calculations of
the optical near-field, generated by evanescent waves close to the surface of
periodic nanostructured arrays, together with advanced atom wavepacket
propagation on this optical potential.Comment: 8 figures, 10 pages, submitted to Phys. Rev.
Weak capture of protons by protons
The cross section for the proton weak capture reaction
is calculated with wave functions obtained from a number of modern, realistic
high-precision interactions. To minimize the uncertainty in the axial two-body
current operator, its matrix element has been adjusted to reproduce the
measured Gamow-Teller matrix element of tritium decay in model
calculations using trinucleon wave functions from these interactions. A
thorough analysis of the ambiguities that this procedure introduces in
evaluating the two-body current contribution to the pp capture is given. Its
inherent model dependence is in fact found to be very weak. The overlap
integral for the pp capture is predicted to be in the range
7.05--7.06, including the axial two-body current contribution, for all
interactions considered.Comment: 17 pages RevTeX (twocolumn), 5 postscript figure
Experimental evidence for 56Ni-core breaking from the low-spin structure of the N=Z nucleus 58Cu
Low-spin states in the odd-odd N=Z nucleus 58Cu were investigated with the
58Ni(p,n gamma)58Cu fusion evaporation reaction at the FN-tandem accelerator in
Cologne. Seventeen low spin states below 3.6 MeV and 17 new transitions were
observed. Ten multipole mixing ratios and 17 gamma-branching ratios were
determined for the first time. New detailed spectroscopic information on the
2+,2 state, the Isobaric Analogue State (IAS) of the 2+,1,T=1 state of 58Ni,
makes 58Cu the heaviest odd-odd N=Z nucleus with known B(E2;2+,T=1 --> 0+,T=1)
value. The 4^+ state at 2.751 MeV, observed here for the first time, is
identified as the IAS of the 4+,1,T=1 state in 58Ni. The new data are compared
to full pf-shell model calculations with the novel GXPF1 residual interaction
and to calculations within a pf5/2 configurational space with a residual
surface delta interaction. The role of the 56Ni core excitations for the
low-spin structure in 58Cu is discussed.Comment: 15 pages, 7 figures, submitted to Phys. Rev.
Dark matter and Colliders searches in the MSSM
We study the complementarity between dark matter experiments (direct
detection and indirect detections) and accelerator facilities (the CERN LHC and
a TeV Linear Collider) in the framework of the
constrained Minimal Supersymmetric Standard Model (MSSM). We show how
non--universality in the scalar and gaugino sectors can affect the experimental
prospects to discover the supersymmetric particles. The future experiments will
cover a large part of the parameter space of the MSSM favored by WMAP
constraint on the relic density, but there still exist some regions beyond
reach for some extreme (fine tuned) values of the supersymmetric parameters.
Whereas the Focus Point region characterized by heavy scalars will be easily
probed by experiments searching for dark matter, the regions with heavy
gauginos and light sfermions will be accessible more easily by collider
experiments. More informations on both supersymmetry and astrophysics
parameters can be thus obtained by correlating the different signals.Comment: 25 pages, 10 figures, corrected typos and reference adde
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