Vegetals as natural sources of antioxidants

VEGETALS AS NATURAL SOURCES OF ANTIOXIDANTS. Growing knowledge on the health-promoting impact of antioxidants in everyday foods, combined with the assumption that a number of common synthetic preservatives may have hazardous side effects has led to increased investigations in the field of natural antioxidants, principally those found in plants. Food industries normally discard plant residues that could benefit the human health and diminish undesirable environmental impact. Once estimated the content of antioxidants in these residues, advantageous economical and social alternatives to the discard are possible, for example, their use for preparation of nutraceuticals to be offered to low-income populations. We present here a broad, although not complete, account of the continuously growing knowledge on the antioxidant capacity of whole fruits, seeds and peels, cereals, vegetal oils and aromatic plants, at several physical forms, as well as a description of the usual methods for evaluating their antioxidant capacity and examples of agroindustrial processes that could be harnessed for the production of antioxidant supplement food, along with research perspectives in the area.Univ Fed Alagoas, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, BrazilUniv Fed Alagoas, Ctr Ciencias Agr, BR-57100000 Rio Largo, AL, BrazilUniv Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ciencias Terra, BR-09972270 Diadema, SP, BrazilUniv Fed Ceara, Dept Quim Organ & Inorgan, BR-60451970 Fortaleza, Ceara, BrazilUniv Fed Sao Paulo, Dept Ciencias Terra, BR-09972270 Diadema, SP, BrazilWeb of Scienc

Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits

Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. the highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. the investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)INCT-RedoxomaINCT-BioanaliticaUniv Fed Alagoas, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, BrazilUniv Pernambuco, BR-56300000 Petrolina, PE, BrazilUniv Fed Alagoas, Rede Nordeste Biotecnol RENORBIO, BR-57072970 Maceio, AL, BrazilUniv Fed Alagoas, Inst Ciencias Biol & Saude, BR-57072970 Maceio, AL, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508000 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilUniv Estadual Campinas, Inst Biol, Dept Biol Vegetal, Campinas, SP, BrazilUniv Fed Ceara, Dept Quim Organ & Inorgan, BR-60021970 Fortaleza, CE, BrazilEmbrapa Tabuleiros Costeiros, Tabuleiro Martins, BR-57061970 Maceio, AL, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema, SP, BrazilFAPESP: 2008/58035-6Web of Scienc

Detecção amperométrica, por microeletrodos, de espécies reativas de oxigênio e nitrogênio em células isoladas estimuladas por substâncias biologicamente ativas

PARIS-BIUSJ-Physique recherche (751052113) / SudocSudocFranceF

Antiparasitic and immunomodulatory activities of 1,1-bis(4-Hydroxyphenyl)-2-Phenyl-but-1-ene and Its protected and free 2-Ferrocenyl derivatives

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-12-02T12:35:05Z No. of bitstreams: 1 Soares MBP Antiparasitic and....pdf: 117652 bytes, checksum: 4a98f792abe5cce85a6ebdd002cba4b1 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2014-12-02T12:35:14Z (GMT) No. of bitstreams: 1 Soares MBP Antiparasitic and....pdf: 117652 bytes, checksum: 4a98f792abe5cce85a6ebdd002cba4b1 (MD5)Made available in DSpace on 2014-12-02T12:46:58Z (GMT). No. of bitstreams: 1 Soares MBP Antiparasitic and....pdf: 117652 bytes, checksum: 4a98f792abe5cce85a6ebdd002cba4b1 (MD5) Previous issue date: 2010Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Engenharia Tecidual e Imunofarmacologia Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, BrasilÉcole Nationale Supérieure de Chimie de Paris. Laboratoire Charles Friedel. Paris, FranceÉcole Nationale Supérieure de Chimie de Paris. Laboratoire Charles Friedel. Paris, FranceInstituto de Química e Biotecnologia. UFAL. Maceió, Al, BrasilInstituto de Química e Biotecnologia. UFAL. Maceió, Al, BrasilÉcole Nationale Supérieure de Chimie de Paris. Laboratoire Charles Friedel. Paris, FranceThe ferrocenyl diphenol 1 [1,1-bis(4-hydroxyphenyl)-2-ferrocenyl-but-1-ene] displays strong cytotoxic effects against a variety of cancer cells. In the present study we have evaluated the immunomodulatory and antiparasitic activities of compound 1 and its protected dipalmitate analogue 2. We have furthermore compared the antiparasitic results of 1 and 2 with the organic analogue, 3 [1,1-bis(4- hydroxyphenyl)-2-phenyl-but-1-ene], where the ferrocenyl group has been replaced by a phenyl ring. When assayed against normal (noncancerous) splenocytes, all compounds were considered nontoxic. Compound 1 inhibited NO production by macrophages, inhibited concanavalin A-induced lymphoproliferation, and was active against Leishmania amazonensis and Trypanosoma cruzi. Compound 2 had lower activity in all assays performed. Surprisingly, compounds 1 and 2 exhibited similar and significant activity against Plasmodium falciparum, with IC50 values of 3.50 and 1.33 mM, respectively. Compound 3 showed an inverse activity profile, being active against T. cruzi but far less active against P. falciparum

Dispositivo Sensor, Processo De Preparação Do Dispositivo, Método Para Detecção De Artemisinina Em Amostras Multicomponentes, E Uso

DISPOSITIVO SENSOR, PROCESSO DE PREPARAÇÃO DO DISPOSITIVO MÉTODO PARA DETECÇÃO DE ARTEMISININA EM AMOSTRAS MULTICOMPONENTES, E USO. A presente invenção tem como objeto proporcionar um dispositivo amperométrico para detecção de artemisinina e o processo de produção do mesmo. O dispositivo é de fácil operação, susceptivel a miniaturização e portabilidade. Esse sensor proporciona diagnosticar alterações dos níveis de artemisinína, in foco de forma mais precisa, rápida, simples e seletiva de que os sistemas atualmente existentes. Em especial, o dispositivo compreende uma base de pasta de carbono, a partir da imobilização de hemina em sílica gel modificada com óxido de titánio, capaz de quantificar níveis baixos de artemisinina (ARN) em uma amostra em especial uma amostra multicomponente como, por exemplo, um isolado, um extrato vegetal, uma fração enriquecida, uma composição fitoterápica, uma composição cosmética e uma composição farmacológica. A presente invenção se situa nos campos da química e farmácia.BRPI0900203 (A2)G01N27/327G01N27/406BR2009PI00203G01N27/327G01N27/40

Antiplasmodial activity of iron(II) and ruthenium(II) organometallic complexes against Plasmodium falciparum blood parasites

Submitted by Nuzia Santos ([email protected]) on 2016-06-22T18:41:14Z No. of bitstreams: 1 ve_Nicolli_Souza_Antiplasmodial_CPqRR_2015.pdf: 340896 bytes, checksum: 9a3a8d863fefeed357c10b19fe7229f7 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-06-22T18:47:32Z (GMT) No. of bitstreams: 1 ve_Nicolli_Souza_Antiplasmodial_CPqRR_2015.pdf: 340896 bytes, checksum: 9a3a8d863fefeed357c10b19fe7229f7 (MD5)Made available in DSpace on 2016-06-22T18:47:32Z (GMT). No. of bitstreams: 1 ve_Nicolli_Souza_Antiplasmodial_CPqRR_2015.pdf: 340896 bytes, checksum: 9a3a8d863fefeed357c10b19fe7229f7 (MD5) Previous issue date: 2015Made available in DSpace on 2016-07-07T11:36:18Z (GMT). No. of bitstreams: 3 ve_Nicolli_Souza_Antiplasmodial_CPqRR_2015.pdf.txt: 43305 bytes, checksum: 262d2aa476d964da2ca1227c8a80deb6 (MD5) ve_Nicolli_Souza_Antiplasmodial_CPqRR_2015.pdf: 340896 bytes, checksum: 9a3a8d863fefeed357c10b19fe7229f7 (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Alagoas. Instituto de Química e Biotecnologia. Maceió, AL, Brasil / Universidade Federal de Alagoas. Faculdade de Nutrição. Maceió, AL, Brasil.Sorbonne Universités. Université Pierre-et-Marie-Curie. Unités Mixtes de Recherche. Paris, France.Sorbonne Universités. Université Pierre-et-Marie-Curie. Unités Mixtes de Recherche. Paris, France / Ecole Nationale Supérieure de Chimie de Paris. Paris, France.Sorbonne Universités. Université Pierre-et-Marie-Curie. Unités Mixtes de Recherche. Paris, France / Ecole Nationale Supérieure de Chimie de Paris. Paris, France.Universidade Federal de Alagoas. Instituto de Química e Biotecnologia. Maceió, AL, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.This work reports the in vitro activity against Plasmodium falciparum blood forms (W2 clone, chloroquine-resistant) of tamoxifen-based compounds and their ferrocenyl (ferrocifens) and ruthenocenyl (ruthenocifens) derivatives, as well as their cytotoxicity against HepG2 human hepatoma cells. Surprisingly with these series, results indicate that the biological activity of ruthenocifens is better than that of ferrocifens and other tamoxifen-like compounds. The synthesis of a new metal-based compound is also described. It was shown, for the first time, that ruthenocifens are good antiplasmodial prototypes. Further studies will be conducted aiming at a better understanding of their mechanism of action and at obtaining new compounds with better therapeutic profile

Total phenolic content and free radical scavenging activities of methanolic extract powders of tropical fruit residues

Methanolic extract powders of acerola, passion fruit and pineapple industrial residues, including pulp, seeds and peel, altogether (except for acerola) devoid of seeds, were screened for antioxidant capacity. the total phenolic contents (TPCs) of the extract powders were compared with their radical-scavenging activities (RSA) against both DPPH(center dot) and superoxide anion (O(2)(center dot-)) radicals, and their protective effect against liposome peroxidation, triggered by peroxyl radical. Lipid peroxidation was followed by the fluorescence decay of the probe, 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C(11)-BODIPY(581/591)). the TPCs of acerola, passion fruit and pineapple extract powders were (94.6 +/- 7.4); (41.2 +/- 4.2) and (9.1 +/- 1.3) mg of gallic acid equivalents g(-1) of dry extract, respectively. Acerola showed the best RSA-DPPH(center dot) scores, whereas passion fruit was more protective on the RSA-O(2)(center dot-) system. Together with the protective effects against lipid peroxidation (rate of BODIPY decay) which, were similar for acerola and passion fruit extracts, these data suggest that the methanolic extracts of acerola and passion fruit residues may be useful as antioxidant supplements, particularly the acerola extract, due to its high phenolic content. (C) 2008 Elsevier B.V. All rights reservedConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Milenio RedoxomaFAPEALBNB (Northeast Bank of Brazil)Univ Fed Alagoas, UFAL, Inst Ciencias Biol & Saude, BR-57010020 Maceio, AL, BrazilUniv Fed Alagoas, UFAL, Inst Quim & Biotecnol, BR-57072970 Maceio, AL, BrazilUniv Fed Alagoas, UFAL, Ctr Ciencias Agr, Lab Solos & Adubacao, BR-57100000 Rio Largo, AL, BrazilUniv São Paulo, Dept Bioquim, Inst Quim, BR-05508000 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilUniv Cruzeiro Sul, Programa Posgrad Ciencias Movimento Humano, BR-08060070 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, BrazilCNPq: 151874/2007-7Web of Scienc

A Very Low Potential Electrochemical Detection Of L-cysteine Based On A Glassy Carbon Electrode Modified With Multi-walled Carbon Nanotubes/gold Nanorods.

A nanohybrid platform built with multi-walled carbon nanotubes and gold nanorods, prepared via a cationic surfactant-containing seed-mediated sequential growth process, in aqueous solution, on a glassy carbon substrate has been successfully developed to be used in the electrocatalytic oxidation of L-cysteine (Cys). The nanohybrid was characterized by transmission electron microscopy, Raman spectroscopy and electrochemical measurements. Cyclic voltammetry results had shown that the modified electrode allows the oxidation of Cys at a very low anodic potential (0.00 V vs. Ag/AgCl). The kinetic constant kcat for the catalytic oxidation of Cys was evaluated by chronoamperometry and provided a value of 5.6×10(4) L mol(-1) s(-1). The sensor presents a linear response range from 5.0 up to 200.0 µmol L(-1), detection limit of 8.25 nmol L(-1) and a sensitivity of 120 nA L µmol(-1).50202-

Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits Antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits

Ethanol extracts of powdered genipap (Genipa americana L), umbu (Spondia tuberosa A.) and siriguela (Spondia purpurea L) prepared from separate pulp, seeds and peel were investigated for their (i) antioxidant capacity, which was evaluated by various known methods; (ii) acetylcholinesterase (AChE) inhibitory activity; and (iii) cytotoxic effect on corneal epithelial cells of sheep. The highest values of total phenolic content were obtained with peel and seed extracts. Siriguela and umbu (seeds and peel) extracts displayed the highest antioxidant activities. Lipid peroxidation assays using mimetic biomembranes and mouse liver homogenates indicated that genipap pulp is a promising antioxidant. The investigation of phenols and organic acid contents revealed the presence of quercetin, citric and quinic acids, chlorogenic acid derivatives, among others, in several extracts, with the highest amount found in siriguela seeds. Genipap pulp and siriguela seed ethanol extracts presented an AChE inhibition zone similar to that of the positive control, carbachol. AChE inhibition assay with chlorogenic acid, one of the main constituents of siriguela seeds, revealed that this acid showed activity similar to that of the control physostigmine. These data suggest that these extracts are potentially important antioxidant supplements for the everyday human diet, pharmaceutical and cosmetic industries. (C) 2012 Elsevier Ltd. All rights reserved.CNPq/PNPDCNPq/PNPDCAPES PNPDCAPES/PNPDCAPES/PROCAD/NFCAPES/PROCAD/NFFAPESP/BIOEN [2008/58035-6]FAPESP/BIOENINCT-RedoxomaINCTRedoxomaINCTBioanaliticaINCT-BioanaliticaFAPEAL/CNPq/PRONEXFAPEAL/CNPq/PRONE