Chitosan-Based Materials: An Overview of Potential Applications in Food Packaging

Chitosan is a multifunctional biopolymer that is widely used in the food and medical fields because of its good antibacterial, antioxidant, and enzyme inhibiting activity and its degradability. The biological activity of chitosan as a new food preservation material has gradually become a hot research topic. This paper reviews recent research on the bioactive mechanism of chitosan and introduces strategies for modifying and applying chitosan for food preservation and different preservation techniques to explore the potential application value of active chitosan-based food packaging. Finally, issues and perspectives on the role of chitosan in enhancing the freshness of food products are presented to provide a theoretical basis and scientific reference for subsequent research

Chitosan-based hydrogels: From preparation to applications, a review

Chitosan, derived from the deacetylation of chitin, is an abundant natural biopolymer on earth. Chitosan and its derivatives have become promising biological materials because of their unique molecular structure and excellent biological activities. The reactive functional groups of chitosan such as the amino and hydroxyl groups play a crucial role in facilitating the synthesis of three-dimensional hydrogel. Chitosan-based hydrogels have been widely used in medical, pharmaceutical, and environmental fields for years. Nowadays, chitosan-based hydrogels have been found in a wide range of applications in the food industry such as food sensors, dye adsorbents and nutrient carriers. In this review, recently developed methods for the preparation of chitosan-based hydrogels were given, and the biological activities of chitosan-based hydrogels were systematically introduced. Additionally, the recent progress in food sensors, packaging, dye adsorbents, and nutrient carriers was discussed. Finally, the challenges and prospects for the future development of chitosan-based hydrogels were discussed

Preparation of Chitosan/β-Cyclodextrin Composite Membrane and Its Adsorption Mechanism for Proteins

A significant portion of the protein in food waste will contaminate the water. The chitosan/modified β-cyclodextrin (CS/β-CDP) composite membranes were prepared for the adsorption of bovine serum albumin (BSA) in this work to solve the problem of poor adsorption protein performance and easy disintegration by a pure chitosan membrane. A thorough investigation was conducted into the effects of the preparation conditions (the mass ratio of CS and β-CDP, preparation temperature, and glutaraldehyde addition) and adsorption conditions (temperature and pH) on the created CS/β-CDP composite membrane. The physical and chemical properties of pure CS membrane and CS/β-CDP composite membrane were investigated. The results showed that CS/β-CDP composite membrane has better tensile strength, elongation at break, Young’s modulus, contact angle properties, and lower swelling degree. The physicochemical and morphological attributes of composite membranes before and after the adsorption of BSA were characterized by SEM, FT-IR, and XRD. The results showed that the CS/β-CDP composite membrane adsorbed BSA by both physical and chemical mechanisms, and the adsorption isotherm, kinetics, and thermodynamic experiments further confirmed its adsorption mechanism. As a result, the CS/β-CDP composite membrane of absorbing BSA was successfully fabricated, demonstrating the potential application prospect in environmental protection

Efficient Biotransformation of Polysialogangliosides for Preparation of GM1 by Cellulosimicrobium sp. 21

A new ganglioside transformed strain isolated from soil was identified as Cellulosimicrobium sp. 21. It produced a sialidase which transformed polysialo-gangliosides GD1 and GT1 into a monosialoterahexosylganglioside, i.e., ganglioside GM1. The sialidase had both NeuAc-α-2,3- and NeuAc-α-2,8-sialidase activity without producing asiolo-GM1. The optimum conditions were evaluated and it was found that the transformation was optimally performed at 30 °C and pH 7.0. The substrate should be added at the beginning of the reaction and the concentration of substrate was 3% (w/v). Under these optimum conditions, Cellulosimicrobium sp. 21 converted GD1 and GT1 into GM1 in inorganic medium in a 5 L bioreactor with the recovery rate of 69.3%. The product contained 50.3% GM1 and was purified on silica to give the product with 95% of GM1 with a recovery rate of 30.5%. Therefore, Cellulosimicrobium sp. 21 has potential to be applied in the production of GM1 in the pharmaceutical industry

Biotransformation of ginsenoside Rb1 for ginsenoside Rd preparation by <i>Lysinibacillus massiliensis</i> No. 24

400-406Bacterium Lysinibacillus massiliensis No. 24 was tested for its ability to transform the major ginsenosides Rb1. The transformation product was identified by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), and its structure was assigned by 13C-NMR. The bacterium was found to specifically transform major ginsenoside Rb1 and ginsenoside Rd was the sole product. The optimal conditions for transforming Rb1 by L. massiliensis No. 24 were determined to be: substrate concentration, 3 mg/mL; temperature, 34&deg;C; pH 10; the time of substrate addition, 48 h; cell concentration, 0.30&times;109 cells/mL; and biotransformation period, 3 d. Under the optimum conditions, the maximum yield of ginsenoside Rd reached 97%. Further, a preparative scale transformation with L. massiliensis No. 24 was performed with 150 mg Rb1 to give a yield of 79%. This bacterium would be potentially applied in the preparation of Rd