Spintronics-based mesoscopic heat engine

We consider a nanowire suspended on two spin-polarized leads and subject to a nonuniform magnetic field. We show that in such a system a temperature drop between leads can significantly affect the nanowire dynamics. In particular, it is demonstrated that under certain conditions the stationary distribution of the mechanical subsystem has Boltzmann form with effective temperature which is smaller than the temperature of the "cold" lead; this seems rather counterintuitive. We also find that the change of the direction of the temperature gradient results in generation of mechanical vibrations rather than heating of the mechanical subsystem.Comment: 5 pages, 3 figure

Superconducting single-mode contact as a microwave-activated quantum interferometer

The dynamics of a superconducting quantum point contact biased at subgap voltages is shown to be strongly affected by a microwave electromagnetic field. Interference among a sequence of temporally localized, microwave-induced Landau-Zener transitions between current carrying Andreev levels results in energy absorption and in an increase of the subgap current by several orders of magnitude. The contact is an interferometer in the sense that the current is an oscillatory function of the inverse bias voltage. Possible applications to Andreev-level spectroscopy and microwave detection are discussed

On Properties of the Isoscalar Giant Dipole Resonance

Main properties (strength function, energy-dependent transition density, branching ratios for direct nucleon decay) of the isoscalar giant dipole resonance in several medium-heavy mass spherical nuclei are described within a continuum-RPA approach, taking into account the smearing effect. All model parameters used in the calculations are taken from independent data. Calculation results are compared with available experimental data.Comment: 12 pages, 2 figure

The development of compartmentation of cAMP signaling in cardiomyocytes: the role of T-Tubules and caveolae microdomains

3′-5′-cyclic adenosine monophosphate (cAMP) is a signaling messenger produced in response to the stimulation of cellular receptors, and has a myriad of functional applications depending on the cell type. In the heart, cAMP is responsible for regulating the contraction rate and force; however, cAMP is also involved in multiple other functions. Compartmentation of cAMP production may explain the specificity of signaling following a stimulus. In particular, transverse tubules (T-tubules) and caveolae have been found to be critical structural components for the spatial confinement of cAMP in cardiomyocytes, as exemplified by beta-adrenergic receptor (β-ARs) signaling. Pathological alterations in cardiomyocyte microdomain architecture led to a disruption in compartmentation of the cAMP signal. In this review, we discuss the difference between atrial and ventricular cardiomyocytes in respect to microdomain organization, and the pathological changes of atrial and ventricular cAMP signaling in response to myocyte dedifferentiation. In addition, we review the role of localized phosphodiesterase (PDE) activity in constraining the cAMP signal. Finally, we discuss microdomain biogenesis and maturation of cAMP signaling with the help of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Understanding these mechanisms may help to overcome the detrimental effects of pathological structural remodeling

Characters of (relatively) integrable modules over affine Lie superalgebras

In the paper we consider the problem of computation of characters of relatively integrable irreducible highest weight modules L over finite-dimensional basic Lie superalgebras and over affine Lie superalgebras g. The problem consists of two parts. First, it is the reduction of the problem to the [bar over g]-module F(L), where [bar over g] is the associated to L integral Lie superalgebra and F(L) is an integrable irreducible highest weight [bar over g]-module. Second, it is the computation of characters of integrable highest weight modules. There is a general conjecture concerning the first part, which we check in many cases. As for the second part, we prove in many cases the KW-character formula, provided that the KW-condition holds, including almost all finite-dimensional g-modules when g is basic, and all maximally atypical non-critical integrable g-modules when g is affine with non-zero dual Coxeter number.Simons Foundation. Postdoctoral Fellowshi

Role of shear stress in endothelial cell morphology and expression of cyclooxygenase isoforms

MEDLINE® is the source for the MeSH terms of this document.Objective-: The goal of this study was to examine the effect of chronic heterogeneous shear stress, applied using an orbital shaker, on endothelial cell morphology and the expression of cyclooxygenases 1 and 2. Methods and results-: Porcine aortic endothelial cells were plated on fibronectin-coated Transwell plates. Cells were cultured for up to 7 days either under static conditions or on an orbital shaker that generated a wave of medium inducing shear stress over the cells. Cells were fixed and stained for the endothelial surface marker CD31 or cyclooxygenases 1 and 2. En face confocal microscopy and scanning ion conductance microscopy were used to show that endothelial cells were randomly oriented at the center of the well, aligned with shear stress nearer the periphery, and expressed cyclooxygenase-1 under all conditions. Lipopolysaccharide induced cyclooxygenase-2 and the production of 6-keto-prostaglandin F1α in all cells. Conclusion-: Cyclooxygenase-1 is expressed in endothelial cells cultured under chronic shear stress of high or low directionality.Peer reviewedSubmitted Versio

GPER limits adverse changes to Ca2+ signalling and arrhythmogenic activity in ovariectomised guinea pig cardiomyocytes

Background: The increased risk of post-menopausal women developing abnormalities of heart function emphasises the requirement to understand the effect of declining oestrogen levels on cardiac electrophysiology and structure, and investigate possible therapeutic targets, namely the G protein-coupled oestrogen receptor 1 (GPER). Methods: Female guinea pigs underwent sham or ovariectomy (OVx) surgeries. Cardiomyocytes were isolated 150-days post-operatively. Membrane structure was assessed using di-8-ANEPPs staining and scanning ion conductance microscopy. Imunnohistochemistry (IHC) determined the localisation of oestrogen receptors. The effect of GPER activation on excitation-contraction coupling mechanisms were assessed using electrophysiological and fluorescence techniques. Downstream signalling proteins were investigated by western blot. Results: IHC staining confirmed the presence of nuclear oestrogen receptors and GPER, the latter prominently localised to the peri-nuclear region and having a clear striated pattern elsewhere in the cells. Following OVx, GPER expression increased and its activation reduced Ca2+ transient amplitude (by 40%) and sarcomere shortening (by 32%). In these cells, GPER activation reduced abnormal spontaneous Ca2+ activity, shortened action potential duration and limited drug-induced early after-depolarisation formation. Conclusion: In an animal species with comparable steroidogenesis and cardiac physiology to humans, we show the expression and localisation of all three oestrogen receptors in cardiac myocytes. We found that following oestrogen withdrawal, GPER expression increased and its activation limited arrhythmogenic behaviours in this low oestrogen state, indicating a potential cardioprotective role of this receptor in post-menopausal women

Coulomb correlations and coherent charge tunneling in mesoscopic coupled rings

We study the effect of a strong electron-electron (e-e) interaction in a system of two concentric one-dimensional rings with incommensurate areas A_1 and A_2, coupled by a tunnel amplitude. For noninteracting particles the magnetic moment (persistent current) m of the many-body ground state and first excited states is an irregular function of the external magnetic field. In contrast, we show that when strong e-e interactions are present the magnetic field dependence of m becomes periodic. In such a strongly correlated system disorder can only be caused by inter-ring charge fluctuations, controllable by a gate voltage. The oscillation period of m is proportional to 1/(A_1 + A_2) if fluctuations are suppressed. Coherent inter-ring tunneling doubles the period when charge fluctuations are allowed.Comment: 4 pages, 4 eps figure

Nanoscale, Voltage-Driven Application of Bioactive Substances onto Cells with Organized Topography

With Scanning Ion Conductance Microscopy (SICM), a non-contact scanning probe technique, it is possible to obtain information about both the surface topography of live cells and to apply molecules onto specific nanoscale structures. The technique is therefore widely used to apply chemical compounds and to study the properties of molecules on the surface of various cell types. The heart muscle cells, the cardiomyocytes, possess a highly elaborate, unique surface topography including T-tubule openings leading into a cell internal system which exclusively har-bors many proteins necessary for the cells physiological function. Here we applied Isoproterenol into these surface openings by changing the applied voltage over the SICM nanopipette. To determine the grade of precision of our application we used finite element simulations to inves-tigate how the concentration profile varies over the cell surface. We first obtained topography scans of the cardiomyocytes using SICM and then determined the electrophoretic mobility of Isoproterenol in a high ion solution to be -7×10-9 m2/Vs. The simulations showed that the delivery to the T-tubule opening is highly confined to the underlying Z-groove and especially to the first T-tubule opening, where the concen-tration is approximately 6.5 times higher compared to on a flat surface under the same delivery settings. Delivery to the crest, instead of the T-tubule opening, resulted in a much lower concentration, emphasizing the importance of topography on agonist delivery. In conclusion SICM, unlike other techniques, can reliably deliver precise quantities of compounds to the T-tubules of cardiomyocyte