Geochemical Dispersion of Elements and Their Correlation with Gold in the Regolith at the Tetteh Prospect of the Chirano Gold Mines in the Sefwi Belt of the Birimian, Southwestern Ghana

The processes controlling gold (Au) mineralization in Chirano, a gold prospect in south-western Ghana have been investigated using a detailed geochemical dataset of 45 variables from 197 sampling locations. The data were subjected to advanced statistical analyses and bivariate graphical interpretations in the light of the underlying lithology. Four processes of significance have been identified from the multivariate analysis in terms of the geochemistry of the soil in the area. The most important process accounts for almost half of the total variance in the dataset and also correlates strongly with Au. Although Arsenic (As) registers the highest loading under this factor, Aluminium (Al), Silver (Ag), Manganese (Mn), Copper (Cu), and Iron (Fe) record higher correlations and could serve as better pathfinders than As in the study area. This study also shows that there is a second, less prominent process contributing to the availability of Au in the area. This process ranks third amongst the processes of significance in terms of the geochemistry, and correlates negatively with As, suggesting that the latter is not suitable as a pathfinder for Au in the traditional sense. However, since this third process is weaker compared to the first, the implication of this interpretation may be limited to only some locations in the study area. In addition to the revelations from the multivariate statistics, the regolith in the study area has evolved over the years and therefore the changes in regolith should be factored into the exploration protocols. The pre-existing preserved surfaces were found to be masked predominantly by transported materials consisting of ferruginized and exotic sediments. Within the surficial regolith and especially in the Fe-oxyhydroxide rich horizons, Au concentrations increase to significant values that are higher than those of the bedrock or saprolite thereby showing the significant Au re-distribution within the regolith. This study also highlights the apparent Au depletion in the mottled clay zones and in the saprolite and the enrichment of the metal in lateritic residuum and the topsoil. The high Au expressions in the upper parts of the regolith generally are transported whereas the residual soil anomalies have low Au concentrations and do not merit further exploration follow up. Keywords: Regolith, Gold mineralization, Pathfinder elements, Chirano mine, Sefwi belt, southwestern Ghana

RBF and Rno promote photoreceptor differentiation onset through modulating EGFR signaling in the Drosophila developing eye

AbstractThe retinoblastoma gene Rb is the prototype tumor suppressor and is conserved in Drosophila. We use the developing fly retina as a model system to investigate the role of Drosophila Rb (rbf) during differentiation. This report shows that mutation of rbf and rhinoceros (rno), which encodes a PHD domain protein, leads to a synergistic delay in photoreceptor cell differentiation in the developing eye disc. We show that this differentiation delay phenotype is caused by decreased levels of different components of the Epidermal Growth Factor Receptor (EGFR) signaling pathway in the absence of rbf and rno. We show that rbf is required for normal expression of Rhomboid proteins and activation of MAP kinase in the morphogenetic furrow (MF), while rno is required for the expression of Pointed (Pnt) and Ebi proteins, which are key factors that mediate EGFR signaling output in the nucleus. Interestingly, while removing the transcription activation function of dE2F1 is sufficient to suppress the synergistic differentiation delay, a mutant form of de2f1 that disrupts the binding with RBF but retains the transcription activation function does not mimic the effect of rbf loss. These observations suggest that RBF has additional functions besides dE2F1 binding that regulates EGFR signaling and photoreceptor differentiation

The soil and plant biogeochemistry sampling design for The National Ecological Observatory Network

Human impacts on biogeochemical cycles are evident around the world, from changes to forest structure and function due to atmospheric deposition, to eutrophication of surface waters from agricultural effluent, and increasing concentrations of carbon dioxide (CO2) in the atmosphere. The National Ecological Observatory Network (NEON) will contribute to understanding human effects on biogeochemical cycles from local to continental scales. The broad NEON biogeochemistry measurement design focuses on measuring atmospheric deposition of reactive mineral compounds and CO2 fluxes, ecosystem carbon (C) and nutrient stocks, and surface water chemistry across 20 eco‐climatic domains within the United States for 30 yr. Herein, we present the rationale and plan for the ground‐based measurements of C and nutrients in soils and plants based on overarching or “high‐level” requirements agreed upon by the National Science Foundation and NEON. The resulting design incorporates early recommendations by expert review teams, as well as recent input from the larger natural sciences community that went into the formation and interpretation of the requirements, respectively. NEON\u27s efforts will focus on a suite of data streams that will enable end‐users to study and predict changes to biogeochemical cycling and transfers within and across air, land, and water systems at regional to continental scales. At each NEON site, there will be an initial, one‐time effort to survey soil properties to 1 m (including soil texture, bulk density, pH, baseline chemistry) and vegetation community structure and diversity. A sampling program will follow, focused on capturing long‐term trends in soil C, nitrogen (N), and sulfur stocks, isotopic composition (of C and N), soil N transformation rates, phosphorus pools, and plant tissue chemistry and isotopic composition (of C and N). To this end, NEON will conduct extensive measurements of soils and plants within stratified random plots distributed across each site. The resulting data will be a new resource for members of the scientific community interested in addressing questions about long‐term changes in continental‐scale biogeochemical cycles, and is predicted to inspire further process‐based research

Functional consequence of the MET-T1010I polymorphism in breast cancer.

Major breast cancer predisposition genes, only account for approximately 30% of high-risk breast cancer families and only explain 15% of breast cancer familial relative risk. The HGF growth factor receptor MET is potentially functionally altered due to an uncommon germline single nucleotide polymorphism (SNP), MET-T1010I, in many cancer lineages including breast cancer where the MET-T1010I SNP is present in 2% of patients with metastatic breast cancer. Expression of MET-T1010I in the context of mammary epithelium increases colony formation, cell migration and invasion in-vitro and tumor growth and invasion in-vivo. A selective effect of MET-T1010I as compared to wild type MET on cell invasion both in-vitro and in-vivo suggests that the MET-T1010I SNP may alter tumor pathophysiology and should be considered as a potential biomarker when implementing MET targeted clinical trials

802GHz integrated horn antennas imaging array

This short paper presents the pattern measurements at 802GHz of a single element in a 256-element integrated horn imaging array. The integrated-horn antenna consists of a dipole-antenna suspended on a 1μm dielectric membrane inside a pyramidal cavity etched in Silicon. The theoretical far-field patterns, calculated using reciprocity and Floquet-modes representation of the free-space field, agree well with the measured far-field patterns at 802GBz. The associated directivity for a 1.40 λ horn aperture, calculated from the measured E and H-plane patterns is 12.3dB±0.2dB. This work demonstrates that high-efficiency integrated-horn antennas are easily scalable to terahertz frequencies and could be used for radio-astronomical and plasma-diagnostic applications.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44548/1/10762_2005_Article_BF01010881.pd

PI3K Pathway Mutations and PTEN Levels in Primary and Metastatic Breast Cancer

The purpose of this work was to determine whether there are differences in PIK3CA mutation status and PTEN protein expression between primary and matched metastatic breast tumors as this could influence patient management. Fifty-micron paraffin sections were used for DNA extraction and 3-micron slides for immunohistochemistry (IHC) and fluorescent in-situ hybridization (FISH). ER, PR and HER2 IHC were repeated in a central laboratory for both primary and metastasis. PTEN levels were assessed by IHC and PI3K pathway mutations detected by a mass spectroscopy-based approach. Median age was 48 years (range, 30 to 83 years). Tumor subtype included 72% hormone receptor-positive/HER2-negative, 20% HER2-positive, and less than 7.8% triple receptor negative. Tissues were available for PTEN IHC in 46 primary tumors and 52 metastases. PTEN was lost in 14 (30%) primary tumors and 13 (25%) metastases. There were 5 cases of PTEN loss and eight cases of PTEN gain from primary to metastasis (26% discordance). Adequate DNA was obtained on 46 primary tumors and on 50 metastases for PIK3CA analysis. PIK3CA mutations were detected in 19 (40%) of primary tumors and 21 (42%) of metastases. There were five cases of PIK3CA mutation loss, and four cases of mutation gain (18% discordance). There was an increase of the level of PIK3CA mutations in four cases, and decrease in one from primary to metastasis. There is a high level of discordance in PTEN level, PIK3CA mutations, and receptor status between primary and metastatic disease that may influence patient selection and response to PI3K-targeted therapies

Gemini Near Infrared Spectrograph - Distant Quasar Survey: Prescriptions for Calibrating UV-Based Estimates of Supermassive Black Hole Masses in High-Redshift Quasars

The most reliable single-epoch supermassive black hole mass ($M_{\rm BH}$) estimates in quasars are obtained by using the velocity widths of low-ionization emission lines, typically the H$\beta$ $\lambda4861$ line. Unfortunately, this line is redshifted out of the optical band at $z\approx1$, leaving $M_{\rm BH}$ estimates to rely on proxy rest-frame ultraviolet (UV) emission lines, such as C IV $\lambda1549$ or Mg II $\lambda2800$, which contain intrinsic challenges when measuring, resulting in uncertain $M_{\rm BH}$ estimates. In this work, we aim at correcting $M_{\rm BH}$ estimates derived from the C IV and Mg II emission lines based on estimates derived from the H$\beta$ emission line. We find that employing the equivalent width of C IV in deriving $M_{\rm BH}$ estimates based on Mg II and C IV provides values that are closest to those obtained from H$\beta$. We also provide prescriptions to estimate $M_{\rm BH}$ values when only C IV, only Mg II, and both C IV and Mg II are measurable. We find that utilizing both emission lines, where available, reduces the scatter of UV-based $M_{\rm BH}$ estimates by $\sim15\%$ when compared to previous studies. Lastly, we discuss the potential of our prescriptions to provide more accurate and precise estimates of $M_{\rm BH}$ given a much larger sample of quasars at $3.20 \lesssim z \lesssim 3.50$, where both Mg II and H$\beta$ can be measured in the same near-infrared spectrum.Comment: 19 pages (AASTeX 6.3.1), 9 figures, accepted for publication in Ap

Strong signature of natural selection within an FHIT intron implicated in prostate cancer risk

Previously, a candidate gene linkage approach on brother pairs affected with prostate cancer identified a locus of prostate cancer susceptibility at D3S1234 within the fragile histidine triad gene (FHIT), a tumor suppressor that induces apoptosis. Subsequent association tests on 16 SNPs spanning approximately 381 kb surrounding D3S1234 in Americans of European descent revealed significant evidence of association for a single SNP within intron 5 of FHIT. In the current study, resequencing and genotyping within a 28.5 kb region surrounding this SNP further delineated the association with prostate cancer risk to a 15 kb region. Multiple SNPs in sequences under evolutionary constraint within intron 5 of FHIT defined several related haplotypes with an increased risk of prostate cancer in European-Americans. Strong associations were detected for a risk haplotype defined by SNPs 138543, 142413, and 152494 in all cases (Pearson's χ2 = 12.34, df 1, P = 0.00045) and for the homozygous risk haplotype defined by SNPs 144716, 142413, and 148444 in cases that shared 2 alleles identical by descent with their affected brothers (Pearson's χ2 = 11.50, df 1, P = 0.00070). In addition to highly conserved sequences encompassing SNPs 148444 and 152413, population studies revealed strong signatures of natural selection for a 1 kb window covering the SNP 144716 in two human populations, the European American (π = 0.0072, Tajima's D= 3.31, 14 SNPs) and the Japanese (π = 0.0049, Fay & Wu's H = 8.05, 14 SNPs), as well as in chimpanzees (Fay & Wu's H = 8.62, 12 SNPs). These results strongly support the involvement of the FHIT intronic region in an increased risk of prostate cancer. © 2008 Ding et al