A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Traduction et handicap ou l'histoire d'un rendez-vous manqué...

Le présent travail de recherche a pour but de soulever une question qui revêt semble-t-il un grand intérêt pour le domaine de la traduction. Nous avons voulu dans notre propos étudier le handicap et son positionnement dans un axe professionnel ainsi que disciplinaire de la traduction. Conscient du caractère quelque peu polémique d'une telle thématique, il apparaît que le sujet du handicap et les particularités qu'il entraîne dans le milieu spécifique de la traduction est un sujet présentant une certaine originalité et ayant par nature une ampleur propre à permettre le traitement de plusieurs aspects connexes du monde professionnel de la traduction et des méthodes d'enseignement de formation pour les traducteurs. Pour ce travail, nous avons décidé de prendre trois axes d'analyse principaux, à savoir le droit, la socio-psychologie et la médecine

Enzymatic characterization of 30 kDa lipase from Pseudomonas aeruginosa ATCC 27853

An extracellular lipase from Pseudomonas aeruginosa ATCC 27853 has been purified and its enzymatic characteristics were determined. According to SDS-PAGE and gel filtration molecular mass estimated to be 30 kDa, what classified the lipase in group I.1. Although 14 lipases from P. aeruginosa with similar molecular mass are referred to date, their basic enzymatic properties have not been reported yet. To address the gap we found: the optimal temperature and pH in water solution being 50 °C and 9.3, respectively; the lipase was inhibited with Hg2+ ions and sodium dodecylsulphate (SDS), while non-ionic detergent Triton X-100 activated the enzyme; the lipase hydrolyzed more rapidly middle chain triglycerides and it was not regiospecific; the lipase demonstrated naturally occurring stability in different organic solvents with concentrations ranging from 30 to 70%, including good thermal stability in 30% organic solvent solution. Even though strain P. aeruginosa ATCC 27853 was not isolated from extreme environment it showed activity in organic solvent suggesting that this lipase is suitable for variety of applications, including reactions in water restricted medium and bioremediation of contaminations by organic solvents

Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase, Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities, and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats

The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.); H: homocysteine 0.45 µmol/g b.w./day s.c.; CPA saline (0.9% NaCl 0.2 mL/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 µmol/g b.w./day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p < 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats

The Effects of Folic Acid Administration on Cardiac Tissue Matrix Metalloproteinases Activities and Hepatorenal Biomarkers in Diabetic Rats

The aims of this study were to investigate the influence of diabetes mellitus (DM) and folic acid treatment on the liver and renal biomarkers, and on heart remodeling through evaluation of cardiac matrix metalloproteinase (MMPs) activities. There were four groups: C – control (physiological saline 1ml/kg, i.p. 28 days), DM – diabetes mellitus (STZ 100mg/kg in physiological saline, i.p. one day), FA – folic acid (5mg/kg in physiological saline, i.p. 28 days), and DM+FA – DM and folic acid group (STZ 100mg/kg in physiological saline, i.p. one day and folic acid 5mg/kg in physiological saline, i.p. 28 days). Our results demonstrated increased aminotransferases, and alkaline phosphatase activities, urea and creatinine and decreased albumin and fibrinogen concentration in the DM group. MMP-2 relative activity was elevated in the DM and FA group, while MMP-9 was decreased in the DM and increased in the FA group. The folic acid treatment of diabetic rats did not change aminotransferases activities; it alleviated the increase in alkaline phosphatase and the decrease in albumin and fibrinogen concentration, and significantly reduced MMP-2 activity; however, it increased urea and creatinine concentrations. In conclusion, folic acid treatment of diabetic rats has cardio- and hepatoprotective effects. Nevertheless, folic acid dosing should be carefully considered because of possible renal damage.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author