Taxol-DNA interactions: fluorescence and CD studies of DNA groove binding properties of taxol

Taxol is perhaps the most successful drug used for the treatment of various cancers. Comprehensive literature accumulated on therapeutics of the drug has indicated numerous side effects. In this paper, by use of fluorescence spectroscopy, it is shown that taxol binds to DNA with an affinity constant (Ka) of 1.08×107 M-1. This binding is accompanied by a large 'red edge excitation shift' (REES) of fluorescence emission maximum in taxol-DNA complex. The results point to an interaction of taxol with its core eight-membered ring in the DNA groove and the three phenyl rings projecting away from the DNA. The drug encompasses about two base pairs of DNA upon binding to it. Systematic studies with taxol analogues confirms such a mode of binding. These interesting findings on hitherto unknown taxol-DNA interactions may have clinical implications in view of its large number of side effects and pharmacokinetics

Preparation, Characterization and in vivo Evaluation of Parenteral Sustained Release Microsphere Formulation of Zopiclone

The aim of this study was to prepare zopiclone-loaded polycaprolactone microspheres by emulsion solvent evaporation technique with different drug-to-carrier ratios {MP 1 (1:1), MP 2 (1:2), MP 3 (1:3), and MP 4 (1:4)}, characterize and evaluate the in vivo performance. The microspheres were characterized for particle size, surface morphology, drug excipient compatibility, percentage yield, drug entrapment, and in vitro release kinetics. Pharmacokinetics and pharmacodynamics were evaluated after parenteral administration so as to determine the sustained action of the drug after one-time administration of the formulation in a rat model. Of four formulations prepared, MP 2, i.e., 1:2 (drug–polymer) ratio was selected as the optimized formulation based on particle size, particle shape, and the release behavior. The size of microspheres was found to be ranging from 5.4 to 12.1 µm. The shape of microspheres was found to be spherical by SEM. Among the four formulations, MP 2 (1:2) showed maximum percentage yield of 75% ± 2.68%. There was no interaction between drug and polymer by FT-IR study. In the in vitro release study, formulation MP 2 (1:2) showed 86.5% drug release and was found to be sustained for 10 days. The microsphere formulations were able to sustain the release of drug both in vitro and in vivo. Pharmacodynamic study (Maze apparatus) indicated that the anxiolytic activity shown by zopiclone microspheres was significant when compared to the zopiclone solution given daily

ESTABLISHMENT OF VICARIOUS CALIBRATION/VALIDATION FACILITY FOR SPACE BORNE HIGH AND MID RESOLUTION OPTICAL SENSORS

Vicarious calibration refers to methods that make use of “invariant” natural targets of the Earth for the post-launch calibration of sensors. This process of calibration is useful for initial phase orbit operation of sensor and thereafter for validation during its operational span. This method of periodic evaluation of radiometric and geometric performance of the space-borne optical sensors and validation of derived radiance/reflectance is to ensure availability of consistent and accurate data products to the user community. This paper describes one such Cal/Val facility’s design, engineering aspects and realization at National Remote Sensing Centre (NRSC), Shadnagar. The target materials used for filling the Cal/Val site are studied for its reflectance. These targets have reflectance ranging from 9% to 53%, in the VNIR (Visible and Near Infra-Red) region. This paper also describes instruments used for calibration and homogeneity studies of targets for its invariance since operationalisation (January 2016). The last section of this paper is about reflectance based absolute radiometric calibration of medium resolution Indian Remote Sensing (IRS) sensor (Resourcesat and Cartosat series) using RT model and edge based LSF/MTF estimation of high resolution sensor carried out using the Cal/Val facility

The Murchison Widefield Array

It is shown that the excellent Murchison Radio-astronomy Observatory site allows the Murchison Widefield Array to employ a simple RFI blanking scheme and still calibrate visibilities and form images in the FM radio band. The techniques described are running autonomously in our calibration and imaging software, which is currently being used to process an FM-band survey of the entire southern sky.Comment: Accepted for publication in Proceedings of Science [PoS(RFI2010)016]. 6 pages and 3 figures. Presented at RFI2010, the Third Workshop on RFI Mitigation in Radio Astronomy, 29-31 March 2010, Groningen, The Netherland

Salmonella enterica Serovar Typhimurium Lacking hfq Gene Confers Protective Immunity against Murine Typhoid

Salmonella enterica is an important enteric pathogen and its various serovars are involved in causing both systemic and intestinal diseases in humans and domestic animals. The emergence of multidrug-resistant strains of Salmonella leading to increased morbidity and mortality has further complicated its management. Live attenuated vaccines have been proven superior over killed or subunit vaccines due to their ability to induce protective immunity. Of the various strategies used for the generation of live attenuated vaccine strains, focus has gradually shifted towards manipulation of virulence regulator genes. Hfq is a RNA chaperon which mediates the binding of small RNAs to the mRNA and assists in post-transcriptional gene regulation in bacteria. In this study, we evaluated the efficacy of the Salmonella Typhimurium Δhfq strain as a candidate for live oral vaccine in murine model of typhoid fever. Salmonella hfq deletion mutant is highly attenuated in cell culture and animal model implying a significant role of Hfq in bacterial virulence. Oral immunization with the Salmonella hfq deletion mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium. Moreover, protection was induced upon both multiple as well as single dose of immunizations. The vaccine strain appears to be safe for use in pregnant mice and the protection is mediated by the increase in the number of CD4+ T lymphocytes upon vaccination. The levels of serum IgG and secretory-IgA in intestinal washes specific to lipopolysaccharide and outer membrane protein were significantly increased upon vaccination. Furthermore, hfq deletion mutant showed enhanced antigen presentation by dendritic cells compared to the wild type strain. Taken together, the studies in murine immunization model suggest that the Salmonella hfq deletion mutant can be a novel live oral vaccine candidate

T-S variability and volume transport in the central Bay of Bengal during southwest monsoon

50-55The variability of temperature and salinity along 11 degrees N and 12 degrees N sections in the Bay of Bengal during southwest monsoon months (July-September) was found to be large in the upper 200 m west of 83 degrees E and cast of 90 degrees E, and up to upper 400 m in the central parts between 84 degrees and 89 degrees E. Coastal upwelling off Madras appears to be intense during August with a strong (40 cm.sec(-1)) northward flow close to the coast. The associated nort hward transport in the upper 100 m (between 81 degrees and 82 degrees E) increased by 2.5 x 10(6) m(3).sec(-1) from July to August. At the location of penetration of Arabian Sea High salinity Watermass (T = 25 degrees C. S = 35.2 x 10(-3). Delta(s,t) = 400 cl/t) doming of isolines of T, S and sigma(t) (sigma(t)) towards sea surface was noticed between 86 degrees and 89 degrees E only during August. The flow in this region was directed towards north with a volume transport of 5.5 x 10(6) m(3) sec(-1) in the upper 100 m