1 research outputs found
New insights on arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two
major inorganic nitrogen forms available for plant growth. Plant preference for one or the other
form depends on the interplay between plant genetic background and environmental variables.
Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate
fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions.
However, ammonium nutrition may become a stressful situation for a wide range of plant species
when the ion is present at high concentrations. Although studied for long time, there is still an
important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition.
In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants
grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with
differential abundance between both conditions. These proteins revealed new potential important
players on root response to ammonium nutrition, such as H+
-consuming metabolic pathways
to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and
glucosinolate biosynthetic pathways