Diversidad genética de Pinus pinaster en la Península Ibérica: correlación entre isoenzimas y caracteres cuantitativos

The estimation of genetic diversity using molecular markers is a major component of genetic conservation programs. However, molecular data are only weakly correlated with adaptive variation, which seriously limits the value of molecular information for guiding conservation policies. In this paper, we used allozyme markers to analyse the distribution of gene diversity in the native range of Pinus pinaster Ait. in the Iberian Peninsula, including seven marginal populations close to the Mediterranean Basin. Then, the variability of three quantitative traits (total height, stem form and survival) was computed using data from a multisite provenance test in central Spain and the two data sets were compared. Within the general pattern of variation, marginal populations presented levels of diversity closely related to those of nearby central populations, clearly suggesting that historical factors were more important than actual population sizes in determining levels of observed diversity. A weak but nevertheless significant correlation between allozymes and quantitative variability was found in maritime pine. Environmental gradients, as measured by geographic variables, are suggested to have selective effects on quantitative traits and to influence effective population size, which might explain the weak correlation found between allozyme and adaptive variability.Uno de los principales componentes de los programas de conservación genética es el uso de marcadores moleculares para la estimación de la diversidad genética. A pesar de ello, la variación observada en marcadores moleculares está débilmente correlacionada con la variación adaptativa, lo que limita seriamente la aplicación de estos estudios en el desarrollo de políticas de conservación. En este trabajo, se compara la distribución de la diversidad genética de Pinus pinaster Ait. en la Península Ibérica obtenida con marcadores moleculares (isoenzimas) y caracteres cuantitativos (altura, forma del fuste y supervivencia). El estudio incluye siete poblaciones marginales. Éstas, muestran valores de diversidad similares a los de poblaciones centrales próximas lo que sugiere una gran importancia de los procesos históricos regionales frente a efectos relacionados con el tamaño efectivo poblacional. La correlación de los marcadores moleculares utilizados con los caracteres cuantitativos medidos en los ensayos de campo es débil, aunque significativa. Esta correlación podría explicarse considerando efectos selectivos en respuesta a gradientes ambientales que afectan a su vez el tamaño efectivo de las poblaciones

A preliminary study on the use of jenny colostrum to improve quality in extenders for freezing donkey semen

Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. Ejaculates were collected from five fertile Donkeys. Sperm samples were pooled, diluted and cryopreserved into three experimental extender groups: BotuCrio®, lactose-extender supplemented with egg yolk (20%) and lactose-extender supplemented with jenny colostrum (20%). The results demonstrated that lactose-jenny colostrum samples displayed significantly higher values in almost all parameters evaluated (p < 0.05) compared with the other two extenders after thawing (BotuCrio® and lactose-egg yolk based extender, respectively) –Total Motility, Viability, HOS test, VCL, VSL and VAP. Acrosome status, LIN, STR and WOB despite showing lower values, none of them were statistically significant (p > 0.05). In conclusion, the extender containing jenny colostrum can be successfully used for donkey semen crypreservation and could effectively improve donkey sperm qualities after freezing -thawing

Optimal selection and location of bess systems in medium-voltage rural distribution networks for minimizing greenhouse gas emissions

This paper explores a methodology to locate battery energy storage systems (BESS) in rural alternating current (AC) distribution networks fed by diesel generators to minimize total greenhouse gas emissions. A mixed-integer nonlinear programming (MINLP) model is formulated to represent the problem of greenhouse gas emissions minimization, considering power balance and devices capabilities as constraints. To model the BESS systems, a linear relationship is considered between the state of charge and the power injection/consumption using a charging/discharging coefficient. The solution of the MINLP model is reached through the general algebraic modeling system by employing the BONMIN solver. Numerical results in a medium-voltage AC distribution network composed of 33 nodes and 32 branches operated with 12.66 kV demonstrate the effectiveness of including BESS systems to minimize greenhouse gas emissions in diesel generators that feeds rural distribution networks

Predictability of the resonance frequency analysis in the survival of dental implants placed in the anterior non-atrophied edentulous mandible

Background: Dental primary implant stability is considered essential in the success of the osseointegration process. The recent advent of the resonance frequency analysis (RFA) seems to effectively measure primary implant stability, although its relationship with implant survival has to be further established. Patients and Methods: Seventy patients with complete mandibular edentulism underwent dental implant rehabilitation by means of the placement of 68 dental implants within the interforaminal region and subsequent placement of an overdenture. Primary implant stability was measured by means of RFA and it was expressed in terms of implant stability quotient (ISQ) on the day of the implant insertion and at the time of the healing abutment placement in a conventional implant two-stage surgical procedure. Results: Overall implant survival rate was 97.1% at the end of the follow-up period. The mean ISQ value for 3.75 and 4.25 mm diameter implants was 78.4 ± 5.46 and 80.83 ± 5.35 respectively, at the time of the implant placement; and 76.68 ± 4.34 and 78.22 ± 6.87 respectively, at the second surgical stage. No statistical differences were observed in relation to changes in mean ISQ value along the healing process Conclusions: No statistical differences in terms of primary and secondary implant stability measured by RFA exists between 3.75 mm and 4.25 mm diameter implants in the conventional implant two-stage surgical procedure in patients with non-atrophied edentulous mandible being restored with an overdenture. Furthermore, no statistical association between RFA and the implant insertion torque was observed for endosseous dental implant placement at the first surgical stage. © Medicina Oral

Effect of Mare Colostrum in Extenders for Freezing Stallion Semen

This study aimed to evaluate the addition of mare colostrum in stallion freezing extenders to improve sperm quality. First, colostrum samples were collected from four mares after the foal''s birth and their composition was determined. Ejaculates were collected from nine fertile stallions. Sperm samples were pooled, diluted, and cryopreserved into three experimental extender groups: Lactose-based extender supplemented with mare colostrum (20%), lactose-based extender supplemented with egg yolk (20%), and BotuCrio. The quality of the post-thaw semen samples were evaluated assessing sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodine (PI) stain, acrosome integrity by fluorescein isothiocyanate and peanut agglutinine (FITC-PNA) and PI stain, plasma membrane functionality by hypo-osmotic swelling (HOS) test, and DNA denaturation by acridine orange (AO) test. There were no significant differences in the percentages of total motility, acrosome integrity, and DNA fragmentation among the extenders after thawing. Kinematics parameters showed significantly higher values in BotuCrio than in lactose extenders (P < .05). BotuCrio and lactose colostrum extender yielded significantly better rates for HOS-test, linearity, straightness, and wobble than egg-yolk extender (P < .05). However, in relation to sperm viability, lactose egg yolk extender showed significantly better results in comparison to the others seminal experimental media (P < .05). In conclusion, the incorporation of mare colostrum into cryopreservation media protected the sperm against cold-shock; therefore, it may be a good cryoprotectant agent alternative in extenders for freezing stallion semen

Influence of 12-week concurrent training on exosome cargo and its relationship with cardiometabolic health parameters in men with obesity

[EN] Exosome release varies depending on the physiological state of the cell, so they could play a fundamental role in obesity, the biggest pandemic in today's societies. The beneficial effects that physical activity has both on weight and cardiovascular parameters may be mediated by exosomes released in response to exercise. Thus, we aimed (I) to study the influence of a 12-week CT intervention on exosome cargo modifications in men with obesity and (II) to determine whether changes in exosomes after the intervention were related to changes in cardiometabolic health parameters in our cohorts. An experimental, controlled design was performed in twelve (nine with valid data) adult male obese patients (mean values: 41.6 years old, 97.6 kg and 32.4 kg/m2) who were randomly divided into a control group (n = 4) and a training group (n = 5), which completed 36 sessions of CT (concurrent training) for 12 weeks. Before and after the training period, cardiometabolic health parameters were evaluated and blood samples to measure exosomes and proteins were drawn. No changes were observed in the levels of any exosomal markers and proteins; however, associations of changes between CD81 and both fat mass and weight, Flot-1 and VO2max, HSP70 and both CRP and left ventricle diastolic diameter or CD14 and leptin were found (all p ≤ 0.05). Although the current CT was not able to clearly modify the exosome cargo, a certain medium to large clinical effect was manifested considering the nature of this study. Moreover, the associations found between the promoted changes in cardiometabolic parameters and exosome-carried proteins could indicate a relationship to be considered for future treatments in patients with obesity.SICurrent research activities of D.J.-P. are supported by a grant from the Spanish Ministry of Science and Innovation—MINECO (RYC-2014-16938) and the EXERNET Research Network on Exercise and Health in Special Populations (DEP2005-00046/ACTI). Technology Fund—FEDER funds (R&D&I Operating Program) was used by and for the benefit of companies (Technology Fund) INNTERCONECTA (BRISA Contract-University of Cádiz). The results of the study are presented clearly, honestly and without fabrication, falsification or inappropriate data manipulation.Biomedical Research Networking Center on Frailty and Healthy Aging (CIBERFES) and FEDER funds from the European Union (CB16/10/00477)

A mixed-integer convex model for the optimal placement and sizing of distributed generators in power distribution networks

The optimal placement and sizing of distributed generators is a classical problem in power distribution networks that is usually solved using heuristic algorithms due to its high complexity. This paper proposes a different approach based on a mixed-integer second-order cone programming (MI-SOCP) model that ensures the global optimum of the relaxed optimization model. Second-order cone programming (SOCP) has demonstrated to be an efficient alternative to cope with the non-convexity of the power flow equations in power distribution networks. Of relatively new interest to the power systems community is the extension to MI-SOCP models. The proposed model is an approximation. However, numerical validations in the IEEE 33-bus and IEEE 69-bus test systems for unity and variable power factor confirm that the proposed MI-SOCP finds the best solutions reported in the literature. Being an exact technique, the proposed model allows minimum processing times and zero standard deviation, i.e., the same optimum is guaranteed at each time that the MI-SOCP model is solved (a significant advantage in comparison to metaheuristics). Additionally, load and photovoltaic generation curves for the IEEE 69-node test system are included to demonstrate the applicability of the proposed MI-SOCP to solve the problem of the optimal location and sizing of renewable generators using the multi-period optimal power flow formulation. Therefore, the proposed MI-SOCP also guarantees the global optimum finding, in contrast to local solutions achieved with mixed-integer nonlinear programming solvers available in the GAMS optimization software. All the simulations were carried out via MATLAB software with the CVX package and Gurobi solver

Effects of hydrogen and primary air in a commercial partially-premixed atmospheric gas burner by means of optical and supervised machine learning techniques

In order to ascertain the effects of the hydrogen addition and the primary air-fuel ratio on burner performance and emissions, we conduct tests on a commercial atmospheric gas burner using pure methane and a blend of hydrogen/methane. Relevant statistical image features are extracted from a UV–VIS camera equipped with narrow-band optical filters. Radical image results agrees with spectrometric data, showing the relevance of the OH* intensity radiation coming from the outer non-premixed zone. The double-cone flame structure is evident, showing a growing secondary non-premixed cone as the primary air-fuel ratio is decreased. In addition, the direct relationship found between flame radical imaging features and NOx emissions has been used to develop a predictive model by integrating classification techniques and neural networks. The research confirms UV–VIS chemiluminescence imaging techniques as powerful tools aimed at combustion monitoring, with huge prospects of being integrated within advanced emission control techniques for commercial burners

LQR-Based adaptive virtual inertia for grid integration of wind energy conversion system based on synchronverter model

This paper proposes adaptive virtual inertia for the synchronverter model implemented in a wind turbine generator system integrated into the grid through a back-to-back converter. A linear dynamic system is developed for the proposed adaptive virtual inertia, which employs the frequency deviation and the rotor angle deviation of the synchronverter model as the state variables and the virtual inertia and frequency droop gain as the control variables. In addition, the proposed adaptive virtual inertia uses a linear quadratic regulator to ensure the optimal balance between fast frequency response and wind turbine generator system stress during disturbances. Hence, it minimizes frequency deviations with minimum effort. Several case simulations are proposed and carried out in MATLAB/Simulink software, and the results demonstrate the effectiveness and feasibility of the proposed adaptive virtual inertia synchronverter based on a linear quadratic regulator. The maximum and minimum frequency, the rate change of the frequency, and the integral of time-weighted absolute error are computed to quantify the performance of the proposed adaptive virtual inertia. These indexes are reduced by 46.61%, 52.67%, 79.41%, and 34.66%, in the worst case, when the proposed adaptive model is compared to the conventional synchronverter model