6 research outputs found
Metal-Induced Production of a Novel Bioadsorbent Exopolysaccharide in a Native Rhodotorula mucilaginosa from the Mexican Northeastern Region
There is a current need to develop low-cost strategies to degrade and eliminate industrially
used colorants discharged into the environment. Colorants discharged into natural water
streams pose various threats, including: toxicity, degradation of aesthetics and inhibiting
sunlight penetration into aquatic ecosystems. Dyes and colorants usually have complex aromatic
molecular structures, which make them very stable and difficult to degrade and eliminate
by conventional water treatment systems. The results in this work demonstrated that
heavy metal-resistant Rhodotorula mucilaginosa strain UANL-001L isolated from the northeast
region of Mexico produce an exopolysaccharide (EPS), during growth, which has colorant
adsorption potential. The EPS produced was purified by precipitation and dialysis and
was then physically and chemically characterized by Scanning Electron Microscopy, Fourier
Transform Infrared Spectroscopy, and chemical elemental analysis. Here, the ability of the
purified EPS produced to adsorb methylene blue (MB), which served as a model colorant, is
studied. MB adsorption by the EPS is found to follow Langmuir Adsorption Isotherm kinetics
at 25°C. Further, by calculating the Langmuir constant the adsorption capabilities of the EPS
produced by the Rhodotorula mucilaginosa strain UANL-001L is compared to that of other
adsorbents, both, microbially produced and from agroindustrial waste. The total adsorption
capacity of the EPS, from the Rhodotorula mucilaginosa strain UANL-001L, was found to be
two-fold greater than the best bioadsorbents reported in the literature. Finally, apart from
determining which heavy metals stimulated EPS production in the strain, the optimal conditions
of pH, heavy metal concentration, and rate of agitation of the growing culture for EPS production, was determined. The EPS reported here has the potential of aiding in the efficient
removal of colorants both in water treatment plants and in situ in natural water streams
Caracterización de la actividad antiamibiana del gosipol
Tesis (Doctorado en Ciencias Especialidad en Microbiología) UANLUANLhttp://www.uanl.mx
Caracterización de la actividad antiamibiana del gosipol / [por]María Teresa González Garza y Barrón.
Age-related yield of adipose-derived stem cells bearing the low-affinity nerve growth factor receptor
Adipose-derived stem cells (ADSCs) are a heterogeneous cell population that may be enriched by positive selection with antibodies against the low-affinity nerve growth factor receptor (LNGFR or CD271), yielding a selective cell universe with higher proliferation and differentiation potential. This paper addresses the need for determining the quantity of ADSCs positive for the CD271 receptor and its correlation with donor's age. Mononuclear cells were harvested from the lower backs of 35 female donors and purified using magnetic beads. Multipotency capacity was tested by the expression of stemness genes and through differentiation into preosteoblasts and adipocytes. A significant statistical difference was found in CD271+ concentrations between defined age intervals. The highest yield was found within women on the 30-40-year-old age range. CD271+ ADSCs from all age groups showed differentiation capabilities as well as expression of typical multipotent stem cell genes. Our data suggest that the amount of CD271+ cells correlates inversely with age. However, the ability to obtain these cells was maintained through all age ranges with a yield higher than what has been reported from bone marrow. Our findings propose CD271+ ADSCs as the primary choice for tissue regeneration and autologous stem cell therapies in older subjects. © 2013 Raquel Cuevas-Diaz Duran et al
Liver fibrosis and protection mechanisms action of medicinal plants targeting apoptosis of hepatocytes and hepatic stellate cells
Following chronic liver injury, hepatocytes undergo apoptosis leading to activation of hepatic stellate cells (HSC). Consequently, activated HSC proliferate and produce excessive extracellular matrix, responsible for the scar formation. The pandemic trend of obesity, combined with the high incidence of alcohol intake and viral hepatitis infections, highlights the urgent need to find accessible antifibrotic therapies. Treatment strategies should take into account the versatility of its pathogenesis and act on all the cell lines involved to reduce liver fibrosis. Medicinal plants are achieving popularity as antifibrotic agents, supported by their safety, cost-effectiveness, and versatility. This review will describe the role of hepatocytes and HSC in the pathogenesis of liver fibrosis and detail the mechanisms of modulation of apoptosis of both cell lines by twelve known hepatoprotective plants in order to reduce liver fibrosis. © 2014 Florent Duval et al
Compilación de Proyectos de Investigacion de 1984-2002
Instituto Politecnico Nacional. UPIICS