Assessment of carotenoid production by Dunaliella salina in different culture systems and operation regimes

The effect of operation regime and culture system on carotenoid productivity by the halotolerant alga Dunaliella salina has been analyzed. Operation strategies tested included batch and semi continuous regime, as well as a two-stage approach run simultaneously in both, open tanks and closed reactor. The best results were obtained with the closed tubular photobioreactor. The highest carotenoid production (328.8 mg carotenoid l−1 culture per month) was achieved with this culture system operated following the two-stage strategy. Also, closed tubular photobioreactor provided the highest carotenoid contents (10% of dry weight) in Dunaliella biomass and β-carotene abundance (90% of total carotenoids) as well as the highest 9-cis to all-trans β-carotene isomer ratio (1.5 at sunrise).Comisión Interministerial de Ciencia y Tecnología IFD 1997-1780IFAPA CO3-125Plan Andaluz de Investigación CVI13

On the Internationalization of CAD Learning Through an English Glossary

Comunicació presentada al XXIX Congreso International INGEGRAF 2019 "La transformación Digital en la Ingeniería Gráfica” (20-21 Junio 2019, Logroño - La Rioja)The internationalization of higher education is an essential factor to improve the quality and efficiency of Spanish universities, providing students with the main skills, and knowledge to interact effectively in an international and multicultural work context as professionals. The internationalization of universities must be a transversal process, not exclusive of its territorial dimension, aimed at advancing towards a society and a knowledge economy that propitiate a solid and stable model of development and growth. To this end, professors in the area of Graphic Expression for Engineering at the Universitat Jaume I (UJI) have developed an online glossary of specific terms in English related to the 3D modelling CAD tools used in Graphic Engineering subjects. This new online tool seeks to train students to increase their technical vocabulary in English and improve their learning and communication skills to face possible collaborations in future European projects. The glossary is introduced weekly to the students during the course. Subsequently, a survey is conducted to the students to verify the effectiveness of the training. This work collects the results and conclusions of this analysis

A Pseudomonas viridiflava-Related Bacterium Causes a Dark-Reddish Spot Disease in Glycine max

A virulent Pseudomonas viridiflava-related bacterium has been identified as a new pathogen of soybean, one of the most important crops worldwide. The bacterium was recovered from forage soybean leaves with dark-reddish spots, and damage on petioles and pods was also observed. In contrast, common bean was not affected

How far the zone of heat-induced transient block extends beyond the lesion during RF catheter cardiac ablation

This is an Accepted Manuscript of an article published by Taylor & Francis in International Journal of Hyperthermia on 02-01-2023, available online: http://www.tandfonline.com/ https://doi.org/10.1080/02656736.2022.2163310[EN] Purpose While radiofrequency catheter ablation (RFCA) creates a lesion consisting of the tissue points subjected to lethal heating, the sublethal heating (SH) undergone by the surrounding tissue can cause transient electrophysiological block. The size of the zone of heat-induced transient block (HiTB) has not been quantified to date. Our objective was to use computer modeling to provide an initial estimate. Methods and materials We used previous experimental data together with the Arrhenius damage index (omega) to fix the omega values that delineate this zone: a lower limit of 0.1-0.4 and upper limit of 1.0 (lesion boundary). An RFCA computer model was used with different power-duration settings, catheter positions and electrode insertion depths, together with dispersion of the tissue's electrical and thermal characteristics. Results The HiTB zone extends in depth to a minimum and maximum distance of 0.5 mm and 2 mm beyond the lesion limit, respectively, while its maximum width varies with the energy delivered, extending to a minimum of 0.6 mm and a maximum of 2.5 mm beyond the lesion, reaching 3.5 mm when high energy settings are used (25 W-20s, 500 J). The dispersion of the tissue's thermal and electrical characteristics affects the size of the HiTB zone by +/- 0.3 mm in depth and +/- 0.5 mm in maximum width. Conclusions Our results suggest that the size of the zone of heat-induced transient block during RFCA could extend beyond the lesion limit by a maximum of 2 mm in depth and approximately 2.5 mm in width.This work was supported by Spanish Ministerio de Ciencia, Innovacion y Universidades/Agencia Estatal de Investigacion IMCIN/AEI/10.13039/ 501100011033 (Grant RTI2018-094357-B-C21).Pérez, JJ.; Berjano, E.; González Suárez, A. (2023). How far the zone of heat-induced transient block extends beyond the lesion during RF catheter cardiac ablation. International Journal of Hyperthermia. 40(1):1-10. https://doi.org/10.1080/02656736.2022.216331011040

In-silico modeling to compare radiofrequency-induced thermal lesions created on myocardium and thigh muscle

[EN] Beating heart (BH) and thigh muscle (TM) are two pre-clinical models aimed at studying the lesion sizes created by radiofrequency (RF) catheters in cardiac ablation. Previous experimental results have shown that thermal lesions created in the TM are slightly bigger than in the BH. Our objective was to use in-silico modeling to elucidate some of the causes of this difference. In-silico RF ablation models were created using the Arrhenius function to estimate lesion size under different energy settings (25 W/20 s, 50 W/6 s and 90 W/4 s) and parallel, 45 degrees and perpendicular catheter positions. The models consisted of homogeneous tissue: myocardium in the BH model and striated muscle in the TM model. The computer results showed that the lesion sizes were generally bigger in the TM model and the differences depended on the energy setting, with hardly any differences at 90 W/4 s but with differences of 1 mm in depth and 1.5 m in width at 25 W/20 s. The higher electrical conductivity of striated muscle (0.446 S/m) than that of the myocardium (0.281 S/m) is possibly one of the causes of the higher percentage of RF energy delivered to the tissue in the TM model, with differences between models of 2-5% at 90 W/4 s, similar to 9% at 50 W/6 s and similar to 10% at 25 W/20 s. Proximity to the air-blood interface (just 2 cm from the tissue surface) artificially created in the TM model to emulate the cardiac cavity had little effect on lesion size. In conclusion, the TM-based experimental model creates fairly similar-sized lesions to the BH model, especially in high-power short-duration ablations (50 W/6 s and 90 W/4 s). Our computer results suggest that the higher electrical conductivity of striated muscle could be one of the causes of the slightly larger lesions in the TM model.This research was funded by the Spanish Ministerio de Ciencia, Innovacion y Universidades/Agencia Estatal de Investigacion MCIN/AEI/10.13039/501100011033 (Grant number: RTI2018-094357-B-C21)Pérez, JJ.; Berjano, E.; González-Suárez, A. (2022). In-silico modeling to compare radiofrequency-induced thermal lesions created on myocardium and thigh muscle. Bioengineering. 9(7):1-13. https://doi.org/10.3390/bioengineering9070329S1139