Effect Of Inhibition Of Nitric Oxide Synthase On Blood Pressure And Renal Sodium Handling In Renal Denervated Rats.

The role of sympathetic nerve activity in the changes in arterial blood pressure and renal function caused by the chronic administration of N G-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, was examined in sham and bilaterally renal denervated rats. Several studies have demonstrated that sympathetic nerve activity is elevated acutely after L-NAME administration. To evaluate the role of renal nerve activity in L-NAME-induced hypertension, we compared the blood pressure response in four groups (N = 10 each) of male Wistar-Hannover rats weighing 200 to 250 g: 1) sham-operated vehicle-treated, 2) sham-operated L-NAME-treated, 3) denervated vehicle-treated, and 4) denervated L-NAME-treated rats. After renal denervation or sham surgery, one control week was followed by three weeks of oral administration of L-NAME by gavage. Arterial pressure was measured weekly in conscious rats by a tail-cuff method and renal function tests were performed in individual metabolic cages 0, 7, 14 and 21 days after the beginning of L-NAME administration. L-NAME (60 mg kg-1 day-1) progressively increased arterial pressure from 108 +/- 6.0 to 149 +/- 12 mmHg (P<0.05) in the sham-operated group by the third week of treatment which was accompanied by a fall in creatinine clearance from 336 +/- 18 to 222 +/- 59 microl min-1 100 g body weight-1 (P<0. 05) and a rise in fractional urinary sodium excretion from 0.2 +/- 0. 04 to 1.62 +/- 0.35% (P<0.05) and in sodium post-proximal fractional excretion from 0.54 +/- 0.09 to 4.7 +/- 0.86% (P<0.05). The development of hypertension was significantly delayed and attenuated in denervated L-NAME-treated rats. This was accompanied by a striking additional increase in fractional renal sodium and potassium excretion from 0.2 +/- 0.04 to 4.5 +/- 1.6% and from 0.1 +/- 0.015 to 1.21 +/- 0.37%, respectively, and an enhanced post-proximal sodium excretion compared to the sham-operated group. These differences occurred despite an unchanged creatinine clearance and Na+ filtered load. These results suggest that bilateral renal denervation delayed and attenuated the L-NAME-induced hypertension by promoting an additional decrease in tubule sodium reabsorption in the post-proximal segments of nephrons. Much of the hypertension caused by chronic NO synthesis inhibition is thus dependent on renal nerve activity.33347-5

Early Detection Of Metabolic And Energy Disorders By Thermal Time Series Stochastic Complexity Analysis.

Maintenance of thermal homeostasis in rats fed a high-fat diet (HFD) is associated with changes in their thermal balance. The thermodynamic relationship between heat dissipation and energy storage is altered by the ingestion of high-energy diet content. Observation of thermal registers of core temperature behavior, in humans and rodents, permits identification of some characteristics of time series, such as autoreference and stationarity that fit adequately to a stochastic analysis. To identify this change, we used, for the first time, a stochastic autoregressive model, the concepts of which match those associated with physiological systems involved and applied in male HFD rats compared with their appropriate standard food intake age-matched male controls (n=7 per group). By analyzing a recorded temperature time series, we were able to identify when thermal homeostasis would be affected by a new diet. The autoregressive time series model (AR model) was used to predict the occurrence of thermal homeostasis, and this model proved to be very effective in distinguishing such a physiological disorder. Thus, we infer from the results of our study that maximum entropy distribution as a means for stochastic characterization of temperature time series registers may be established as an important and early tool to aid in the diagnosis and prevention of metabolic diseases due to their ability to detect small variations in thermal profile.4770-

Maternal Undernutrition And The Offspring Kidney: From Fetal To Adult Life.

Maternal dietary protein restriction during pregnancy is associated with low fetal birth weight and leads to renal morphological and physiological changes. Different mechanisms can contribute to this phenotype: exposure to fetal glucocorticoid, alterations in the components of the renin-angiotensin system, apoptosis, and DNA methylation. A low-protein diet during gestation decreases the activity of placental 11ß-hydroxysteroid dehydrogenase, exposing the fetus to glucocorticoids and resetting the hypothalamic-pituitary-adrenal axis in the offspring. The abnormal function/expression of type 1 (AT1(R)) or type 2 (AT2(R)) AngII receptors during any period of life may be the consequence or cause of renal adaptation. AT1(R) is up-regulated, compared with control, on the first day after birth of offspring born to low-protein diet mothers, but this protein appears to be down-regulated by 12 days of age and thereafter. In these offspring, AT2(R) expression differs from control at 1 day of age, but is also down-regulated thereafter, with low nephron numbers at all ages: from the fetal period, at the end of nephron formation, and during adulthood. However, during adulthood, the glomerular filtration rate is not altered, due to glomerulus and podocyte hypertrophy. Kidney tubule transporters are regulated by physiological mechanisms; Na(+)/K(+)-ATPase is inhibited by AngII and, in this model, the down-regulated AngII receptors fail to inhibit Na(+)/K(+)-ATPase, leading to increased Na(+) reabsorption, contributing to the hypertensive status. We also considered the modulation of pro-apoptotic and anti-apoptotic factors during nephrogenesis, since organogenesis depends upon a tight balance between proliferation, differentiation and cell death.431010-

Sublethal effects of chlorantraniliprole and thiamethoxam seed treatments when Lysiphlebus testaceipes feed on sunflower extrafloral nectar

Citation: Moscardini, V. F., Gontijo, P. C., Michaud, J. P., & Carvalho, G. A. (2014). Sublethal effects of chlorantraniliprole and thiamethoxam seed treatments when Lysiphlebus testaceipes feed on sunflower extrafloral nectar. Retrieved from http://krex.ksu.eduThe extrafloral nectar (EFN) of sunflower, Helianthus annuus L., is an important summer resource for many insects and represents a potential route of exposure to systemic insecticides applied as seed treatments to cultivated varieties. Among the many parasitoids that utilize sunflower EFN, Lysiphlebus testaceipes (Cresson) (Hymenoptera: Braconidae) is an important generalist parasitoid of cereal aphids in North America. This study evaluated the performance of adult wasps fed EFN of sunflower plants grown from seed treated with chlorantraniliprole and thiamethoxam. Consumption of EFN from treated sunflower seedings caused no lethal effects, but reduced the numbers of greenbug nymphs, Schizaphis graminum Rondani, attacked and parasitized when wasps foraged in Petri dish arenas. Whereas control females self-superparasitized every fourth host, those exposed to chlorantraniliprole did not. Offspring developmental time and adult emergence were unaffected by either treatment, but thiamethoxam greatly reduced the proportion of female offspring

Non-target effects of chlorantraniliprole and thiamethoxam on Chrysoperla carnea when employed as sunflower seed treatments

Citation: Gontijo, P. C., Moscardini, V. F., Michaud, J. P., & Carvalho, G. A. (2014). Non-target effects of chlorantraniliprole and thiamethoxam on Chrysoperla carnea when employed as sunflower seed treatments. Retrieved from http://krex.ksu.eduThe use of systemic insecticides as seed treatments has raised concern about the possible impacts of these products on natural enemies. This study assessed the effects of sunflower seed treatments with chlorantraniliprole and thiamethoxam on Chrysoperla carnea by exposing larvae and adults to sunflower stem segments grown from treated seeds and the nectar secreted by their extrafloral nectaries. Confinement of larvae with stem segments for their entire developmental period had no effect on their survival or any life history parameter, except that the sex ratio of resulting adults was lower in the thiamethoxam treatment than in chlorantraniliprole. However, when adult pairs of C. carnea were exposed to treated stem segments during their maturation period, their subsequent survival and fecundity was significantly reduced by both materials, with thiamethoxam reducing median survival (LT[subscript 50]) and fecundity to a greater degree than chlorantraniliprole. Insufficient offspring were obtained from adults exposed to thiamethoxam to permit assessment of their fitness, but the offspring in the chlorantraniliprole-exposed adults had reduced larval survival relative to controls. The greater impact of seed treatments on adult lacewings may be partly attributable to their greater consumption of extra-floral nectar. Our results indicate that seed treatment with systemic insecticides can cause negative effects on beneficial insects, potentially disrupting their population dynamics, and should not be assumed compatible with biological control and IPM simply because this mode of application limits direct exposure

Efeito do teor de matéria seca, na ocasião da colheita, na quantidade e na qualidade da silagem.

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Anxiety-like behavior and structural changes of the bed nucleus of the stria terminalis (BNST) in gestational protein-restricted male offspring

Animal evidence has suggested that maternal emotional and nutritional stress during pregnancy is associated with behavioral outcomes in offspring. The nature of the stresses applied may differ, but it is often assumed that the mother's hippocampus-hypothalamic-pituitary-adrenal (HHPA) axis response releases higher levels of glucocorticoid hormones. The bed nucleus of the stria terminalis (BNST) is in a pivotal position to regulate the HHPA axis and the stress response, and it has been implicated in anxiety behavior. In the current study, to search whether BNST structural changes and neurochemical alterations are associated with anxiety-related behavior in adult gestational protein-restricted offspring relative to an age-matched normal protein diet (NP) rats, we conduct behavioral tests and, BNST dendritic tree analysis by Sholl analysis, associated to immunoblotting-protein quantification [11β-HSD2, GR, MR, AT1R, 5HT1A and 5HT2A, corticotrophin-releasing factor (CRH) and CRH1]. Dams were maintained either on isocaloric standard rodent chow [with NP content, 17% casein or low protein content (LP), 6% casein] chow throughout their entire pregnancy. Here, in rats subjected to gestational protein restriction, we found: (a) a significant reduction in dendritic length and impoverished dendritic arborization in BNST neurons; (b) an elevated plasmatic corticosterone levels; and (c) associated with enhanced anxiety-like behavior when compared with age-matched NP offspring. Moreover, altered protein (11β-HSD2, GR, MR and type 1 CRH receptors) expressions may underlie the increase in anxiety-like behavior in LP offspring. This work represents the first demonstration that BNST developmental plasticity by maternal protein restriction, resulting in fine structural changes and neurochemical alterations that are associated with modified behavioral states.Fundação de Amparo à Pesquisa do Estado de São Paulo (2005/54362-4 and 2013/12486-5) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)info:eu-repo/semantics/publishedVersio

Produção de gramíneas do gênero Brachiaria sob níveis de sombreamento.

O trabalho foi realizado com o objetivo de avaliar a produção de B. decumbens cv. Basilisk e B. brizantha, cultivares Marandu e Xaraés, sob diferentes níveis de sombreamento. Utilizou-se delineamento em blocos casualizados com esquema de parcelas subdivididas, considerando os níveis de sombreamento (0, 50, 70%) como parcela e as espécies ou cultivares como subparcelas. Sementes das gramíneas foram postas a germinar em bandejas e transplantadas (três plântulas por vaso). Foram realizados quatro cortes em cada subparcela. Antes de cada corte, mediram-se a altura de planta e a área foliar de quatro folhas em cada planta e quantificou-se o número de perfilhos vivos por vaso. Após a colheita, as plantas foram separadas em lâmina, colmo + bainha e material morto para determinação da produção de matéria seca. Para produção de matéria seca total, calcularam-se a produção média em cada um os cortes e a produção total. No último corte, avaliou-se a massa seca de raízes. As interações significativas foram desdobradas adequadamente. O fator qualitativo foi submetido à comparação de médias pelo teste Tukey e o quantitativo à análise de regressão linear. À exceção da produção média em quatro cortes e da produção de colmos e de material morto, observou-se interação significativa para todas as outras variáveis. Independentemente do nível de sombreamento, o cultivar Xaraés destacou-se positivamente na maioria das características analisadas, enquanto, no nível de 50% de sombreamento, o capim-braquiária apresentou maior produtividade, em porcentagem de produção a pleno sol. Na avaliação da porcentagem de folhas, destacou-se o capim-xaraés, seguido do capim-marandu. Independentemente da forrageira, o nível de sombreamento teve efeito direto sobre o número de perfilhos/planta, a produção de MS do sistema radicular, a área foliar e o valor SPAD

[clinical Relationship Between Chagas' Disease And Primary Arterial Hypertension At An Outpatient Referral Service].

To evaluate the clinical relationship between Chagas' disease and primary arterial hypertension. The study involved 878 chronic chagasic outpatients followed by the Chagas' Disease Study Group of the University Hospital (Unicamp), over a 15-year period. Initially, the age, gender, race, clinical form of Chagas' disease and the presence of hypertension were noted for each patient. Subsequently, the data for matched hypertensive and normotensive chagasic patients were compared. Of the chagasic patients 37% were hypertensive, of those, 65% had some form of heart disease compared to 49% in the normotensive group. Of those patients with Chagas' disease and arterial hypertension, 41% were blacks and 35% were caucasians. Fifty percent of the hypertensive chagasic patients were over 45 years old compared to only 29% of the non-hypertensive patients. Most of the hypertensive chagasic patients were 45 or more years old and showed some degree of heart failure compared to the normotensive group.70431-

Sistema de Integração Lavoura-Pecuária: o modelo implantado na Embrapa Milho e Sorgo.

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