Application of pressure-sensitive paints to unsteady and high-speed flows

The Pressure-Sensitive Paint (PSP) technique allows the global pressure mapping of surfaces under aerodynamic conditions. The present study involves the application of Tris- Bathophenanthroline Ruthenium Perchlorate based PSP, developed in-house, to two different cases; a) the flow through a sonic nozzle, and b) the examination of the effect of dimples on glancing shock wave turbulent boundary layer interactions at transonic speeds

Comparative host specificity of human- and pig- associated Staphylococcus aureus clonal lineages.

Bacterial adhesion is a crucial step in colonization of the skin. In this study, we investigated the differential adherence to human and pig corneocytes of six Staphylococcus aureus strains belonging to three human-associated [ST8 (CC8), ST22 (CC22) and ST36(CC30)] and two pig-associated [ST398 (CC398) and ST433(CC30)] clonal lineages, and their colonization potential in the pig host was assessed by in vivo competition experiments. Corneocytes were collected from 11 humans and 21 pigs using D-squame® adhesive discs, and bacterial adherence to corneocytes was quantified by a standardized light microscopy assay. A previously described porcine colonization model was used to assess the potential of the six strains to colonize the pig host. Three pregnant, S. aureus-free sows were inoculated intravaginally shortly before farrowing with different strain mixes [mix 1) human and porcine ST398; mix 2) human ST36 and porcine ST433; and mix 3) human ST8, ST22, ST36 and porcine ST398] and the ability of individual strains to colonize the nasal cavity of newborn piglets was evaluated for 28 days after birth by strain-specific antibiotic selective culture. In the corneocyte assay, the pig-associated ST433 strain and the human-associated ST22 and ST36 strains showed significantly greater adhesion to porcine and human corneocytes, respectively (p<0.0001). In contrast, ST8 and ST398 did not display preferential host binding patterns. In the in vivo competition experiment, ST8 was a better colonizer compared to ST22, ST36, and ST433 prevailed over ST36 in colonizing the newborn piglets. These results are partly in agreement with previous genetic and epidemiological studies indicating the host specificity of ST22, ST36 and ST433 and the broad-host range of ST398. However, our in vitro and in vivo experiments revealed an unexpected ability of ST8 to adhere to porcine corneocytes and persist in the nasal cavity of pigs

Fundamental and high-order anapoles in all-dielectric metamaterials via Fano-Feshbach modes competition

One of the most fascinating possibilities enabled by metamaterials is the strong reduction of the electromagnetic scattering from nanostructures. In dielectric nanoparticles, the formation of a minimal scattering state at specific wavelengths is associated with the excitation of photonic anapoles, which represent a peculiar type of radiationless state and whose existence has been demonstrated experimentally. In this work, we investigate the formation of anapole states in generic dielectric structures by applying a Fano–Feshbach projection scheme, a general technique widely used in the study of quantum mechanical open systems. By expressing the total scattering from the structure in terms of an orthogonal set of internal and external modes, defined in the interior and in the exterior of the dielectric structure, respectively, we show how anapole states are the result of a complex interaction among the resonances of the system and the surrounding environment. We apply our approach to a circular resonator, where we observe the formation of higher-order anapole states, which are originated by the superposition of several internal resonances of the system

Dynamic transcription programs during ES cell differentiation towards mesoderm in serum versus serum-freeBMP4 culture

Expression profiling of embryonic stem (ES) cell differentiation in the presence of serum has been performed previously. It remains unclear if transcriptional activation is dependent on complext growth factor mixtures in serum or whether this process is intrinsic to ES cells once the stem cell program has been inactivated. The aims of this study were to determine the transcriptional programs associated with the stem cell state and to characterize mesoderm differentiation between serum and serum-free culture

Adaptive-mutation compact genetic algorithm for dynamic environments

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link

Deep Learning to Improve the Sustainability of Agricultural Crops Affected by Phytosanitary Events: A Financial-Risk Approach

open access article.Given the challenges in reducing greenhouse gases (GHG), one of the sectors that have attracted the most attention in the Sustainable Development Agenda 2030 (SDA-2030) is the agricultural sector. In this context, one of the crops that has had the most remarkable development worldwide has been oil-palm cultivation, thanks to its high productive potential and being one of the most efficient sources of palmitic acid production. However, despite the significant presence of oil palm in the food sector, oil-palm crops have not been exempt from criticism, as its cultivation has developed mainly in areas of ecological conservation around the world. This criticism has been extended to other crops in the context of the Sustainable Development Goals (SDG) due to insecticides and fertilisers required to treat phytosanitary events in the field. To reduce this problem, researchers have used unmanned aerial vehicles (UAVs) to capture multi-spectral aerial images (MAIs) to assess fields’ plant vigour and detect phytosanitary events early using vegetation indices (VIs). However, detecting phytosanitary events in the early stages still suggests a technological challenge. Thus, to improve the environmental and financial sustainability of oil-palm crops, this paper proposes a hybrid deep-learning model (stacked–convolutional) for risk characterisation derived from a phytosanitary event, as suggested by lethal wilt (LW). For this purpose, the proposed model integrates a Lagrangian dispersion model of the backward-Gaussian-puff-tracking type into its convolutional structure, which allows describing the evolution of LW in the field for stages before a temporal reference scenario. The results show that the proposed model allowed the characterisation of the risk derived from a phytosanitary event, (PE) such as lethal wilt (LW), in the field, promoting improvement in agricultural environmental and financial sustainability activities through the integration of financial-risk concepts. This improved risk management will lead to lower projected losses due to a natural reduction in insecticides and fertilisers, allowing a balance between development and sustainability for this type of crop from the RSPO standards

A culture-independent method for studying transfer of IncI1 plasmids from wild-type Escherichia coli in complex microbial communities

IncI1 plasmids play a central role in the transfer of antimicrobial resistance genes among Enterobacteriaceae in animals and humans. Knowledge on the dynamics of IncI1 plasmid transfer is limited, mainly due to lack of culture-independent methods that can quantify donor strain survival and plasmid transfer in complex microbial communities. The aim of this study was to develop a culture-independent method to study the dynamics of IncI1 plasmids transfer by fluorescence-activated cell sorting. We genetically modified three wild-type Escherichia coli of animal (n = 2) and human (n = 1) origin carrying blaCMY-2 or blaCTX-M-1 on two epidemic IncI1 plasmids (pST12 and pST7). Non-coding regions on the chromosome and on the IncI1 plasmid of each strain were tagged with mCherry (red) and GFPmut3 (green) fluorescent proteins, respectively, using lambda recombineering. A gene cassette expressing mCherry and lacIq was inserted into the chromosome, whereas the plasmid was marked with a GFPmut3 cassette with LacIq repressible promoter. Therefore, gfpmut3 was repressed in donor strains but expressed in recipient strains acquiring the plasmids. We demonstrated that genetic engineering of the strains did not affect the growth rate and plasmid transfer-ability in filter and broth matings. A proof-of-concept experiment using the CoMiniGut, an in vitro model of the colon, proved the validity of our method for studying the survival of wild-type E. coli and horizontal transfer of IncI1 plasmids under different pH and oxygen conditions. The dual-labeling method by fluorescent proteins is useful to determine persistence of exogenous E. coli and transfer dynamics of IncI1 plasmids in microbial communities

Nonadiabatic Dynamics of Atoms in Nonuniform Magnetic Fields

Dynamics of neutral atoms in nonuniform magnetic fields, typical of quadrupole magnetic traps, is considered by applying an accurate method for solving nonlinear systems of differential equations. This method is more general than the adiabatic approximation and, thus, permits to check the limits of the latter and also to analyze nonadiabatic regimes of motion. An unusual nonadiabatic regime is found when atoms are confined from one side of the z-axis but are not confined from another side. The lifetime of atoms in a trap in this semi-confining regime can be sufficiently long for accomplishing experiments with a cloud of such atoms. At low temperature, the cloud is ellipsoidal being stretched in the axial direction and moving along the z-axis. The possibility of employing the semi-confining regime for studying the relative motion of one component through another, in a binary mixture of gases is discussed.Comment: 1 file, 17 pages, RevTex, 2 table