LncRNA CRNDE inhibits cardiomyocytes apoptosis by YAP1 in myocardial ischaemia/reperfusion injury

Background Cardiomyocytes apoptosis is the basic pathological process of myocardial ischaemia/reperfusion (MI/R) injury, so inhibiting apoptosis of cardiomyocytes can effectively improve MI/R injury. Long non-coding RNA colorectal neoplasia differentially expressed (lncRNA CRNDE) can inhibit cell apoptosis, but its specific role in MI/R injury has not been studied. The aim of this study is to explore the specific effect of lncRNA CRNDE on cardiomyocytes apoptosis. Methods MI/R model in vivo and hypoxia/re-oxygenation (H/R) model in vitro were constructed. Apoptotic levels were assessed by TUNEL staining assay. QRT-PCR was used to validate lncRNA CRNDE level in myocardial tissues and HL-1 cells. The protein expressions of YAP1, Bcl-2 and cleaved caspase-3 were detected by western blot analysis. Flow cytometry was used to determine the apoptosis rate of cardiomyocytes. RIP assay was used to detect the interaction between lncRNA CRNDE and YAP1. Results The extent of cardiomyocytes apoptosis was significantly increased, and the levels of lncRNA CRNDE, YAP1 and Bcl-2 were down-regulated, while cleaved caspase-3 expression was up-regulated in MI/R mice and H/R-treated HL-1 cells. The expressions of YAP1 and Bcl-2 were decreased, while the expression of cleaved caspase-3 was increased after the knockdown of lncRNA CRNDE. Furthermore, lncRNA CRNDE could bind to YAP1 and regulated the protein level of YAP1 by ubiquitination and proteasomal degradation pathway. After transfection of Si-YAP1 in the H/R-treated HL-1 cells transfected with pc-DNA CRNDE, the protein level of Bcl-2 was decreased, while cleaved caspase-3 expression and the apoptosis rate were increased. Conclusion Our study suggested that lncRNA CRNDE could regulate YAP1 level by ubiquitination and proteasomal degradation pathway, thus inhibiting cardiomyocytes apoptosis in MI/R injury

Troxerutin Protects Against Myocardial Ischemia/Reperfusion Injury Via Pi3k/Akt Pathway in Rats

Background/Aims: Troxerutin, also known as vitamin P4, has been commonly used in the treatment of chronic venous insufficiency (CVI) disease. However, its effect on in vivo myocardial ischemia/reperfusion (I/R) injury, a model that closely mimics acute myocardial infarction in humans, is still unknown. Methods: The myocardial I/R injury rat model was created with troxerutin preconditioning. Myocardial infarct size was evaluated by the Evans blue-TTC method. Hemodynamic parameters, including the heart rate (HR), left ventricular end-diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), maximal rate of rise in blood pressure in the ventricular chamber (+dp/dt max), and maximal rate of decline in blood pressure in the ventricular chamber (-dp/dt max) were monitored. Serum TNF-α and IL-10 were determined by ELISA kit. Cell apoptosis was detected by MTT method. Results: Troxerutin preconditioning significantly reduced myocardial infarct size, improved cardiac function, and decreased the levels of creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in the I/R injury rat model. The serum and mRNA levels of TNF-α and IL-10 as well as some apoptosis markers (Bax, Caspase 3) also decreased. Moreover, troxerutin pretreatment markedly increased the phosphorylation of Akt, and blocking PI3K activity by LY294002 abolished the protective effect of troxerutin on I/R injury. Conclusion: Troxerutin preconditioning protected against myocardial I/R injury via the PI3K/Akt pathway

In Vivo Photoacoustic Detection and Imaging of Peroxynitrite

Photoacoustic detection is an emerging noninvasive and nonionizing detection technique with the merits of rich contrast, high resolution, and deep tissue penetration, especially for in vivo detection and imaging. Herein, we developed a photoacoustic (PA) molecular imaging probe (denoted as nanonaps) composed of a naphthalocyanine dye and a heptamethine dye as the internal standard with unchanged signals at 860 nm and the sensing component with peroxynitrite (ONOO^–) target-decreased signals at 775 nm, respectively. The as-prepared nanonaps displayed high sensitivity and specificity of ONOO^– both in vitro and in vivo. The PA₈₆₀/PA₇₇₅ ratio was increased as a function of the concentration of ONOO^– (0–250 nM). More interestingly, our ratiometric nanonaps could be used for in vivo detection and imaging of ONOO^–

Original Sedimentary Pattern of an Inverted Basin: A Case Study from the Bozhong Depression, Offshore Bohai Bay Basin

The third member of Shahejie Formation (Sha-3 member; 42–38Ma of Eocene) in the Bozhong Depression, offshore Bohai Bay Basin was subject to multiple post-depositional modifications. The present structural framework of the Bozhong Depression, which is characterized by sags alternating with uplifts, does not reflect its original sedimentary pattern. Previous studies have not discussed the post-depositional modification of this succession, including the sedimentary pattern variations and the depositional geodynamic setting. This work determined the characteristics of the post-depositional modification and original sedimentary pattern of the Bozhong Depression through analysis of seismic data, well-log data and fission-track ages. The results demonstrate that the Shijiutuo rise, a major structural feature of the current basin, did not exist during the major depositional stage of the Sha-3 member, when the Qinnan sag was largely connected to the Bozhong sag to form a single contiguous deposition area within the basin. By contrast, the Shaleitian and Chengbei rises, located in the western part of the Bozhong Depression, have existed before the depositional period of the Eocene Sha-3 member; these features were manifested as syn-depositional tilted fault blocks, the uplifted footwall blocks of which provided sediments for the neighboring Shanan and Chengbei sags. The western part of the Bonan low rise, located in the southern part of the Bozhong Depression, did not experience uplifting during the depositional phase of the Eocene Sha-3 member. The Huanghekou sag was connected with the Bozhong sag in the western part of the Bozhong Depression. The original sedimentary boundary of the southern Miaoxi sag possibly extended eastward about 10 km and connected with the Bozhong sag at its northern part. The present-day Bodong low rise, which is bounded by the Tan–Lu fault zone, also formed after the depositional period of Eocene Sha-3 member. It is thus concluded that the Bozhong Depression formed a connected large-scale sub-basin during the depositional stage of the Eocene Sha-3 member. Several neighboring sags that are now separated by rises, including the Qinnan, Shanan, Chengbei, Huanghekou, Miaoxi and Bodong sags, formed a single contiguous depositional area during the Eocene. The significant differences between the present and original basin patter and framework provide valuable information for better understanding the history of basin inversion and its impact on related hydrocarbon-system evolution