Levels of Selenomonas Species in Generalized Aggressive Periodontitis

Aim To compare the levels of Selenomonas sputigena and uncultivated/unrecognized Selenomonas species in subgingival biofilms from generalized aggressive periodontitis (GAgP) and periodontaly healthy (PH) subjects. Material and Methods GAgP (n=15) and PH (n=15) subjects were recruited and their clinical periodontal parameters were evaluated. Subgingival plaque samples were collected (9 samples/subject) and analyzed for the levels of 10 bacterial taxa, including cultivated and uncultivated/unrecognized microorganisms using the RNA-oligonucleotide quantification technique (ROQT). Differences in the levels of the test taxa between groups were sought using the Mann-Whitney test. Results GAgP subjects showed significantly higher mean counts of Porphyromonas gingivalis, Selenomonas sputigena and Selenomonas oral clone CS002 (Human Oral Microbial Database (HOMD) Oral Taxon 131), while Actinomyces gerencseriae and Streptococcus sanguinis were found in higher mean counts in PH subjects (pSelenomonas EW084 (HOMD OT 146) was only detected in the GAgP group. In the GAgP group, levels of P. gingivalis and S. sputigena were higher in sites with probing depth (PD) ≥5mm than in shallow sites (PD ≤3mm) (pP. gingivalis (r=0.77; pS. sputigena (r=0.60; pSelenomonas sp. EW076 (OT 139) (r=042, p\u3c0.05). Conclusion S. sputigena, Selenomonas sp. oral CS002 (OT 131) and Selenomonas sp. oral clone EW084 (OT 146) may be associated with the pathogenesis of GAgP, and their role in the onset and progression of this infection should be further investigated

Some peculiarities of motion of neutral and charged test particles in the field of a spherically symmetric charged object in General Relativity

We propose the method of investigation of radial motions for charged and neutral test particles in the Reissner-Nordstr\"{o}m field by means of mass potential. In this context we analyze special features of interaction of charges and their motions in General Relativity and construct the radial motion classification. For test particles and a central source with charges $q$ and $Q$, respectively, the conditions of attraction (when $qQ>0$) and repulsion (when $qQ<0$) are obtained. The conditions of motionless test particle states with respect to the central source are investigated and, in addition, stability conditions for such static equilibrium states are found. It is shown that stable states are possible only for the bound states of weakly charged particles in the field of a naked singularity. Frequencies of small oscillations of test particles near their equilibrium positions are also found.Comment: 15 pages, 9 figure

Impacto da adubação orgânica sobre a incidência de tripes em cebola.

Analisou-se a relação entre adubação orgânica e a incidência de Thrips tabaci Lind. em cebola (Allium cepa L), na EE de Ituporanga,entre agosto e dezembro de 1998. Os tratamentos foram determinados de acordo com a necessidade de N para a cultura pela análise de solo. Empregou-se como fonte orgânica diversos adubos fornecendo 75 Kg/ha de N (esterco suíno; adubo Barriga Verde proveniente de esterco de aves; composto orgânico; esterco de peru; húmus); 37,5 Kg/ha de N (metade da dose normal com esterco de suíno); as testemunhas foram adubação mineral fornecendo 30-120-60 kg/ha de N-P2O5-K2O e o dobro da dose (60-240-120 kg/ha de N-P2O5-K2O); e testemunha sem adubação. Nenhum tratamento apresentou incidência de T. tabaci superior à testemunha sem adubo. A adubação mineral em relação à orgânica não favoreceu significativamente a incidência de T. tabaci . O processo de conversão do manejo do solo da área experimental de convencional para orgânico pode ter favorecido a infestação similar do inseto entre tratamentos. No período de maior incidência de T. tabaci, a relação com nutrientes foi descrita por um modelo envolvendo K/Zn, B e N de maneira positiva. A correlação entre nutrientes e T. tabaci não foi linear na maioria das avaliações. A adubação orgânica pode substituir a adubação mineral na cultura da cebola, pois foi possível atingir níveis de produtividade similares para ambos tratamentos

Genetic signatures of parental contribution in black and white populations in Brazil

Two hundred and three individuals classified as white were tested for 11 single nucleotide polymorphisms plus two insertion/deletions in their Y-chromosomes. A subset of these individuals (n = 172) was also screened for sequences in the first hypervariable segment of their mitochondrial DNA (mtDNA). In addition, complementary studies were done for 11 of the 13 markers indicated above in 54 of 107 black subjects previously investigated in this southern Brazilian population. The prevalence of Y-chromosome haplogroups among whites was similar to that found in the Azores (Portugal) or Spain, but not to that of other European countries. About half of the European or African mtDNA haplogroups of these individuals were related to their places of origin, but not their Amerindian counterparts. Persons classified in these two categories of skin color and related morphological traits showed distinct genomic ancestries through the country. These findings emphasize the need to consider in Brazil, despite some general trends, a notable heterogeneity in the pattern of admixture dynamics within and between populations/groups

Estudos sobre a nutrição mineral do sorgo granífero: VII absorção de sulfato marcado por raizes destacadas

Some aspects of SO4² absorption by excised roots of "Sorghum bicolor (L.) Moench" namely, inibition effects of NaF, KCN, 2,4- DNP and factors such as aeration, solution pH and temperature on the rate of absorption were studied. The excised roots showed multiphasic mecanism of SO4²- absorption. The relative absorption rates, presented by three varieties, were in decreasing order: Tey-101, Contibrasil-101 and Dekalb-E-57. Inhibition effects on the absorption of SO4²- by excised roots were shown in the case of the three ihibitors studied hereof-. Aeration and solution temperature are important factors on SO4²- absorption. At the external solutio n concentration of Na,2SO(4)10-³M the best fitted equation to the experimental values was shown to be: umoles/g dry matter = 1,639- 1,585 exp. (-0,006618t)Um estudo da absorção de sulfato por raizes destacadas de sorgo granífero mostrou: mecanismo (ou sítios) múltiplo para absorção; o tamanho do espaço livre aparente; a influência da temperatura, pH; a dependência da atividade metabólica