On the magnetic structure and wind parameter profiles of Alfven wave driven winds in late-type supergiant stars

Cool stars at giant and supergiant evolutionary phases present low velocity and high density winds, responsible for the observed high mass-loss rates. Although presenting high luminosities, radiation pressure on dust particles is not sufficient to explain the wind acceleration process. Among the possible solutions to this still unsolved problem, Alfven waves are, probably, the most interesting for their high efficiency in transfering energy and momentum to the wind. Typically, models of Alfven wave driven winds result in high velocity winds if they are not highly damped. In this work we determine self-consistently the magnetic field geometry and solve the momentum, energy and mass conservation equations, to demonstrate that even a low damped Alfven wave flux is able to reproduce the low velocity wind. We show that the magnetic fluxtubes expand with a super-radial factor S>30 near the stellar surface, larger than that used in previous semi-empirical models. The rapid expansion results in a strong spatial dilution of the wave flux. We obtained the wind parameter profiles for a typical supergiant star of 16 M_sun. The wind is accelerated in a narrow region, coincident with the region of high divergence of the magnetic field lines, up to 100 km/s. For the temperature, we obtained a slight decrease near the surface for low damped waves, because the wave heating mechanism is less effective than the radiative losses. The peak temperature occurs at 1.5 r_0 reaching 6000 K. Propagating outwards, the wind cools down mainly due to adiabatic expansion.Comment: to appear in the MNRA

Experimental study of the director pattern dynamics in the vicinity of the Fréedericksz twist geometry

We present an experimental study of the transient periodic structures appearing in the nematic director field in the magnetically induced reorientation of the director in the vicinity of the twist Fréedericksz geometry. Thin nematic samples (50 mm thick) were exposed to magnetic fields of variable intensity and orientation relative to the surface aligning direction of the sample. The director reorientation was induced by a rapid rotation of the sample in the static magnetic field producing a misalignment between the director and the magnetic field. The director field was optically monitored during the reorientation process and the transient periodic structures were characterized. Two types of periodic structures could be identified, namely bands and walls. Walls grow from bands close to the twist Fréedericksz geometry. The time dependence of the wave length and inclination of the periodic structures was obtained as a function of the magnetic field intensity and orientation relative to the surface aligning direction of the sample. The results for the bands are compared with the predictions of a model that we specifically developed to account for the non-orthogonal field orientations. It is seen that our model can account rather well for the experimental results considering that it uses only the field rotation time as adjustable parameter. All other model parameters are known

Modeling retrovirus production for gene therapy. 2. Integrated optimization of bioreaction and downstream processing

In this work a model envisaging the integrated optimization of bioreaction and downstream processing is presented. This model extends the work presented in part 1 of this pair of papers by adding ultrafiltration to process optimization. The new operational parameters include ultrafiltration time, pressure, and stirring rate. For global optimization, the model uses as constraints the final product titer and quality to be achieved after downstream processing. This extended model was validated with the same system used in part 1, i.e., PA317 cells producing a recombinant retrovirus containing the LacZ gene as a marker in stirred tanks using porous supports. Optimization of the extended model led to the conclusion that bioreaction should have two steps, batch and perfusion, similar to what was found in part 1. Ultrafiltration in a stirred cell should be performed at low pressures and stirring rates to reduce the losses of infective retroviruses. Sensitivity analysis performed on the results of the integrated optimization showed that under optimal conditions the productivity is less sensitive to the parameters related to ultrafiltration than to those associated with bioreaction. These results were interpreted as reflecting the high yield of ultrafiltration (90%). The relevance of the model extension to perform integrated optimization was also demonstrated since a restriction in the specific ultrafiltration area in downstream processing conditioned perfusion duration and perfusion rate in bioreaction. This clearly indicates that overall process optimization cannot be achieved without integrated optimization

Poly(dimethyl siloxane) surface modification with biosurfactants isolated from probiotic strains

Abstract: Depending on the final application envisaged for a given biomaterial, many surfaces must be modified before use. The material performance in a biological environment is mainly mediated by its surface properties that can be improved using suitable modification methods. The aim of this work was to coat poly(dimethyl siloxane) (PDMS) surfaces with biosurfactants (BSs) and to evaluate how these compounds affect the PDMS surface properties. BSs isolated from four probiotic strains (Lactococcus lactis, Lactobacillus paracasei, Streptococcus thermophilus A, and Streptococcus thermophilus B) were used. Bare PDMS and PDMS coated with BSs were characterized by contact angle measurements, infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The influence of the surface modifications on the materials blood compatibility was studied through thrombosis and hemolysis assays. The cytotoxicity of these materials was tested against rat peritoneal macrophages. AFM results demonstrated the successful coating of the surfaces. Also, by contact angle measurements, an increase of the coated surfaces hydrophilicity was seen. Furthermore, XPS analysis indicated a decrease of the silicon content at the surface, and ATR-FTIR results showed the presence of BS characteristic groups as a consequence of the modification. All the studied materials revealed no toxicity and were found to be nonhemolytic. The proposed approach for the modification of PDMS surfaces was found to be effective and opens new possibilities for the application of these surfaces in the biomedical field