Synthesis of fructo-oligosaccharides using grape must and sucrose as raw materials

Grape must market has been rising and there is an increasing interest to use it as a natural replacement for traditional sugars. Food or beverages with prebiotic compounds, including fructo-oligosaccharides (FOS), emerge as an alternative for the new health style trend. The aim of this work was to investigate whether the combination of grape must with sucrose was a suitable raw material for the synthesis of FOS. This way, a prebiotic syrup containing fructose and FOS, potentially useful for the formulation of foods and beverages, could be obtained. The main process consisted of three stages, namely conditioning of grape must (oxidation of the initial glucose concentration, stage 1), synthesis of FOS [incorporation of 20, 30 and 55% (w/w) sucrose, and 3.5% v/v Viscozyme L − 4.2 U/mg-, stage 2], and conditioning of the final product (oxidation of the glucose generated during the synthesis, stage 3). At stage 1, glucose concentration decreased from 222.8 mg/mL to 47.2 mg/mL, representing a decay of about 80% regarding the initial concentration of glucose. At stage 2, incorporating 20% (w/w) sucrose was not enough to impulse FOS synthesis. In turn, although 30 and 55% (w/w) sucrose produced very similar concentrations of total FOS (DP3 + DP4), 55% (w/w) sucrose led to higher glucose generation and less DP4 formation. Hence, 30% (w/w) sucrose was the condition selected for the synthesis and further conditioning of the obtained product (stage 3). In these conditions, the final product consisted of more than 30% of short chain FOS (19% and 13% of DP3 and DP4, respectively), 55% fructose and less than 11% of glucose and sucrose. Considering that fructose has approximately double sweetening power than glucose, the obtained syrup has a bigger sweetening power in comparison with the original grape must, also providing the prebiotic benefits of FOS.Fil: Ureta, María Micaela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Romano, Nelson Gastón. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Kakisu, Emiliano Javier. Universidad Nacional de Lanús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez Zavaglia, Andrea. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentin

Innovation and Change in Jesuit Education: Horizon 2020, a Case Study in the Jesuit School Network in Catalonia, Spain

Innovation has taken place in education as a necessity to respond to a rapidly changing world. In their commitment to contribute to the creative and healing mission of the Society of Jesus, the Jesuit School Network of Catalonia created HORIZON 2020 to envision a new school to effectively educate in the 21st Century and adapt the Ignatian tradition to the present day. Understanding the case and extracting the main lessons from it were best accomplished by using a qualitative research approach. Particularly, this study used descriptive single-case design with embedded units. The project Horizon 2020 of the Jesuit Education Foundation was the bounded case chosen by its exemplarity in Jesuit education. Data was extracted from four different sources -interviews (individual and focus groups), arcHIVal records, direct observations and documents- and was analyzed in a narrative, interpretive and meaning making level. Four themes emerged from the analysis as important pieces of the transformation: communication, culture of care (cura personalis), decision-making, and participation. Each theme is described from different perspectives and forces that drove the change and mobilized people to promote or oppose it. The power dynamics that coexisted within the organization were an important finding that explained the tensions within the emerging themes. This empirical research analyzes how adaptive leadership takes place in school settings through multifaceted lenses enriched by theories of innovation, design thinking in education and the characteristics of Jesuit Education. Its results can be used by those interested in leadership and educational transformation. This study offers insights for practitioners in school administration and advances the systematic knowledge in leadership

Cultivating sustainable cannabis in the Danish Medicinal Cannabis Pilot Programme : a case study at OC CARE ApS

In my research I explore the Danish Medicinal Cannabis Pilot Programme (Pilot Programme). The study was done with the case of OC Care ApS’ (OC Care) Envision Concept, a legislation-compliant growth facility with a controlled environment, designed to produce sustainable medicinal cannabis. OC Care was chosen as the single case for this study because they are the only licensed company with the aim to produce organic cannabis within the Pilot Programme. OC Care’s Envision Concept is analyzed through an agroecological perspective, by considering the economic, environmental, and social components that together make a system. To understand the context and set the boundaries for the study, the Pilot Programme is analyzed through system thinking methodology. To reach a holistic understanding of the case and its context, data was gathered through multiple sources: participant observation, informal conversations, and semi-structured interviews. Findings indicate that the Pilot Programme has a cautious approach towards legalization, and aims to produce scientific evidence to validate the benefits of medicinal cannabis and regulate its cultivation, processing, and distribution. Due to its pharmaceutical paradigm, hybrid framework, and Denmark’s competitive advantages, Denmark is becoming a leader in the European medicinal cannabis market. To produce organic and sustainable medicinal cannabis OC Care have developed the Envision Concept, a controlled environment cultivation and processing facility, compliant with the European Union’s (EU) Volume 4 EU Guidelines for Good Manufacturing Practice for Medicinal Products for Human and Veterinary Use. The Envision Concept is a system that integrates economic, environmental, and social interests to provide patients with an organic, standardized, and sustainable pharma-grade product.M-A

Biogeochemical Changes During Bio-cementation Mediated by Stimulated and Augmented Ureolytic Microorganisms.

Microbially Induced Calcite Precipitation (MICP) is a bio-mediated cementation process that can improve the engineering properties of granular soils through the precipitation of calcite. The process is made possible by soil microorganisms containing urease enzymes, which hydrolyze urea and enable carbonate ions to become available for precipitation. While most researchers have injected non-native ureolytic bacteria to complete bio-cementation, enrichment of native ureolytic microorganisms may enable reductions in process treatment costs and environmental impacts. In this study, a large-scale bio-cementation experiment involving two 1.7-meter diameter tanks and a complementary soil column experiment were performed to investigate biogeochemical differences between bio-cementation mediated by either native or augmented (Sporosarcina pasteurii) ureolytic microorganisms. Although post-treatment distributions of calcite and engineering properties were similar between approaches, the results of this study suggest that significant differences in ureolysis rates and related precipitation rates between native and augmented microbial communities may influence the temporal progression and spatial distribution of bio-cementation, solution biogeochemical changes, and precipitate microstructure. The role of urea hydrolysis in enabling calcite precipitation through sustained super-saturation following treatment injections is explored

Sexual Function in Women with Breast Cancer: An Evidence Map of Observational Studies

Breast cancer; Psychosexual disorders; Sexual functionCáncer de mama; Trastornos psicosexuales; Función sexualCàncer de mama; Trastorns psicosexuals; Funció sexualBreast cancer (BC) is the leading cause of cancer in women, and has implications for sexual function (SF). In this study, we used an evidence map to identify, describe, and organise the current available evidence regarding SF in women with BC. We searched the MEDLINE, PsycINFO, and CINAHL databases for observational studies assessing SF in women with BC published in English, Spanish, Portuguese, and French between 2000 and 2021 (sample ≥ 50 women). Of the 64 included studies (13,257 women with BC), 58 were published since 2010. Women who were married, partnered, or in relationships represented 74.1% of the entire sample. Only a single study was conducted on women representing a sexual minority. We identified 22 assessment instruments and 40 sexual dysfunction (SdF) domains. The number of publications on SF in women with BC has increased in the last 10 years, but still remains low. Some groups of women are underrepresented, and some SdF domains are underdiagnosed, with the assessment instrument used affecting which domains are studied. Women with BC need to be better screened, as their quality of life (QoL) is affected by SdF

Optimization and evaluation of surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid

Cerebrospinal fluid (CSF) potentially carries an archive of peptides and small proteins relevant to pathological processes in the central nervous system (CNS) and surrounding brain tissue. Proteomics is especially well suited for the discovery of biomarkers of diagnostic potential in CSF for early diagnosis and discrimination of several neurodegenerative diseases. ProteinChip surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is one such approach which offers a unique platform for high throughput profiling of peptides and small proteins in CSF. In this study, we evaluated methodologies for the retention of CSF proteins < 20 kDa in size, and identify a strategy for screening small proteins and peptides in CSF. ProteinChip array types, along with sample and binding buffer conditions, and matrices were investigated. By coupling the processing of arrays to a liquid handler reproducible and reliable profiles, with mean peak coefficients of variation < 20%, were achieved for intra- and inter-assays under selected conditions. Based on peak m/z we found a high degree of overlap between the tested array surfaces. The combination of CM10 and IMAC30 arrays was sufficient to represent between 80–90% of all assigned peaks when using either sinapinic acid or α-Cyano-4-hydroxycinnamic acid as the energy absorbing matrices. Moreover, arrays processed with SPA consistently showed better peak resolution and higher peak number across all surfaces within the measured mass range. We intend to use CM10 and IMAC30 arrays prepared in sinapinic acid as a fast and cost-effective approach to drive decisions on sample selection prior to more in-depth discovery of diagnostic biomarkers in CSF using alternative but complementary proteomic strategies

Coarsening in potential and nonpotential models of oblique stripe patterns

We study the coarsening of two-dimensional oblique stripe patterns by numerically solving potential and nonpotential anisotropic Swift-Hohenberg equations. Close to onset, all models exhibit isotropic coarsening with a single characteristic length scale growing in time as $t^{1/2}$. Further from onset, the characteristic lengths along the preferred directions $\hat{x}$ and $\hat{y}$ grow with different exponents, close to 1/3 and 1/2, respectively. In this regime, one-dimensional dynamical scaling relations hold. We draw an analogy between this problem and Model A in a stationary, modulated external field. For deep quenches, nonpotential effects produce a complicated dislocation dynamics that can lead to either arrested or faster-than-power-law growth, depending on the model considered. In the arrested case, small isolated domains shrink down to a finite size and fail to disappear. A comparison with available experimental results of electroconvection in nematics is presented.Comment: 13 pages, 13 figures. To appear in Phys. Rev.

Concert recording 2019-11-16a

[Track 1]. Concert etude / Alexander Goedicke -- [Track 2]. Concerto in F minor. I. Allegro moderato [Track 3]. II. Adagio religioso, allegretto III. Rondo / Oskar Bohme -- [Track 4]. Four miniatures. I. Fanfare [Track 5]. II. Intermezzo [Track 6]. III. Canto [Track 7]. IV. Tarantella / Joseph Turrin -- [Track 8]. Albumblatt / Alexander Glasunow -- [Track 9]. Sonata, op. 18. I. Allegro con brio [Track 10]. II. Andante con espressione [Track 11]. III. Allegro con anima / Thorvald Hansen -- [Track 12]. The maid of the mist / Herbert L. Clarke

The diverse evolutionary paths of simulated high-z massive, compact galaxies to z=0

Massive quiescent galaxies have much smaller physical sizes at high redshift than today. The strong evolution of galaxy size may be caused by progenitor bias, major and minor mergers, adiabatic expansion, and/or renewed star formation, but it is difficult to test these theories observationally. Herein, we select a sample of 35 massive, compact galaxies ($M_* = 1-3 \times 10^{11}$ M$_\odot$, $M_*/R^{1.5} > 10^{10.5}$ M$_\odot$/kpc$^{1.5}$) at $z=2$ in the cosmological hydrodynamical simulation Illustris and trace them forward to $z=0$ to uncover their evolution and identify their descendants. By $z=0$, the original factor of 3 difference in stellar mass spreads to a factor of 20. The dark matter halo masses similarly spread from a factor of 5 to 40. The galaxies' evolutionary paths are diverse: about half acquire an ex-situ envelope and are the core of a more massive descendant, a third survive undisturbed and gain very little mass, 15% are consumed in a merger with a more massive galaxy, and a small remainder are thoroughly mixed by major mergers. The galaxies grow in size as well as mass, and only $\sim$10% remain compact by $z=0$. The majority of the size growth is driven by the acquisition of ex-situ mass. The most massive galaxies at $z=0$ are the most likely to have compact progenitors, but this trend possesses significant dispersion which precludes a direct linkage to compact galaxies at $z=2$. The compact galaxies' merger rates are influenced by their $z=2$ environments, so that isolated or satellite compact galaxies (which are protected from mergers) are the most likely to survive to the present day.Comment: 19 pages, 10 figures, MNRAS accepted version including 2 new figure