Comparison of copper concentrations in liver and muscle of Squalius cephalus and Capoeta capoeta gracilis (Pisces: Teleostei) in Tajan River, Iran

The aim of this study was to determine and compare the concentration of copper (Cu) in liver and muscle of two fish species Squalius cephalus and Capoeta capoeta gracilis. The fish were caught in four sampling sites along Tajan River, Mazandaran Province, northern Iran. The concentration of Cu was determined using flame atomic absorption spectrometry. There were significant differences (P<0.05) between the sampling sites with regard to Cu concentration in both muscle and liver of fish species.Significant differences were also found (P<0.05) between S. cephalus and C. c. gracilis regarding Cu content in liver and muscle tissues. The content of Cu in the liver was greater (P<0.05) than that in the muscle in both species. Also, Cu content of liver in both species was higher than the standard limits declared by WHO. This study reports high loads of Cu in Tajan River and a high content of Cu in the muscle and liver of S. cephalus and C. c. gracilis

Polymorphism of Pro12Ala in the peroxisome proliferator-activated receptor 2 gene in Iranian diabetic and obese subjects

Background: Peroxisome proliferator-activated receptor 2 (PPAR2) is a nuclear receptor that regulates adipocyte differentiation, lipid metabolism, and insulin sensitivity. The aim of this study was to investigate the association between the Pro12Ala single nucleotide polymorphism (SNP) at the PPAR2 gene and type II diabetes (T2DM) and obesity in an Iranian population. Methods: The genomic DNA of the 312 subjects included four groups: (1) nonobese with type II diabetes, (2) obese without type II diabetes, (3) obese with type II diabetes, and (4) nondiabetic nonobese controls. The Pro12Ala polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: Frequencies of the Ala allele in obese subjects were significantly different from those control subjects (odds ratio [OR], 2.358; 95% confidence interval [CI], 1.101-5.05) (P = 0.025). In contrast, no significant association was detected between the Pro12Ala polymorphism and type II diabetes (OR, 0.652; 95% CI, 0.261-1.628). In all subjects, the Ala carriers had a higher body mass index (BMI) compared with the common allele. Conclusions: Our results showed that the Pro12Ala polymorphism in the PPAR2 gene is associated with obesity in Iranian subjects and the presence of the Ala allele could predict higher BMI. © 2009 Mary Ann Liebert, Inc

Multi-period Project Portfolio Selection under Risk considerations and Stochastic Income

This paper deals with multi-period project portfolio selection problem. In this problem, the available budget is invested on the best portfolio of projects in each period such that the net profit is maximized. We also consider more realistic assumptions to cover wider range of applications than those reported in previous studies. A novel mathematical model is presented to solve the problem, considering risks, stochastic incomes, and possibility of investing extra budget in each time period. Due to the complexity of the problem, an effective meta-heuristic method hybridized with a local search procedure is presented to solve the problem. The algorithm is based on genetic algorithm (GA), which is a prominent method to solve this type of problems. The GA is enhanced by a new solution representation and well selected operators. It also is hybridized with a local search mechanism to gain better solution in shorter time. The performance of the proposed algorithm is then compared with well-known algorithms, like basic genetic algorithm (GA), particle swarm optimization (PSO), and electromagnetism-like algorithm (EM-like) by means of some prominent indicators. The computation results show the superiority of the proposed algorithm in terms of accuracy, robustness and computation time. At last, the proposed algorithm is wisely combined with PSO to improve the computing time considerably

Lethal and sublethal effects of dinotefuran and thiamethoxam on the population growth parameters of the green lacewing, Chrysoperla carnea (Neu.: Chrysopidae), under laboratory conditions

The green lacewing, Chrysoperla carnea (Stephens), is a common natural enemy of various agricultural pestsand widely used as a biocontrol agent in integrated pest management (IPM) programs. The lethal and sublethal effects of the insecticides dinotefuran and thiamethoxam on the first instar larvae of C. carnea was assessed in the laboratory conditions at 25 ± 1°C, 60 ± 5% RH and a photoperiod of 16: 8 (L: D). The LC50 values for dinotefuran and thiamethoxam were 19.382 and 9.880 mg ai/l, respectively, that showed the high toxicity of thiamethoxam on the first instar larvae of C. carnea. To assess the sublethal effects, the first instar larvae were treated with the LC30 for dinotefuran and thiamethoxam at 3.532 and 1.692 mg ai/l, respectively. The estimated rm values in the control, dinotefuran and thiamethoxam were 0.185, 0.186 and 0.143 day-1, respectively. Finite rate of increase (λ) in the control, dinotefuran and thiamethoxam were 1.204, 1.204 and 1.154 day-1. Generation time and doubling time values in the control, dinotefuran and thiamethoxam were 30.77, 30.46 and 35.14 as well as 3.73, 3.72 and 4.82 days, respectively. The gross and net reproductive rates in the control, dinotefuran and thiamethoxam were 459.89, 439.08 and 309.42, and also 298.01, 278.45 and 155.03 (female/female/generation), respectively. Dinotefuran caused no significant adverse effects on the population growth parameters of C. carnea. If similar results are obtained for dinotefuran in the field, it might be an insecticide with low toxicity to C. carnea by using the reduced doses of the insecticide in IPM context. Studies under the laboratory conditions can help us to select some insecticides for additional studies under more natural conditions and for application of suitable insecticides along with natural enemies in pest management

Characterization and prognostic value of mutations in exons 5 and 6 of the p53 gene in patients with colorectal cancers in central Iran

Background/Aims: We aimed to investigate the relation-ships among various mutations of the p53 gene and their protein products, histological characteristics, and disease prognosis of primary colorectal cancer in Isfahan, central Iran. Methods: Sixty-one patients with colorectal adenocarcinoma were enrolled in the study. Mutations of the p53 gene were detected by single-stranded conformation polymorphism and DNA sequencing. The protein stability was evaluated by immunohistochemistry. Patients were followed up to 48 months. Results: Twenty-one point mutations in exons 5 and 6 were detected in the tumor specimens of 14 patients (23%). Of those, 81% and 9.5% were missense and nonsense mutations, respectively. There were also two novel mutations in the intronic region between exons 5 and 6. In 11 mutated specimens, protein stability and protein accumulation were identified. There was a relationship between the type of mutation and protein accumulation in exons 5 and 6 of the p53 gene. The presence of the mutation was associated with an advanced stage of cancer (trend, p&#60;0.009). Patients with mutated p53 genes had significantly lower survival rates than those with wild type p53 genes (p&#60;0.01). Conclusions: Mutations in exons 5 and 6 of the p53 gene are common genetic alterations in colorectal adenocarcinoma in central Iran and are associated with a poor prognosis of the disease

Genetic Variants of Cytochrome b-245, Alpha Polypeptide Gene and Premature Acute Myocardial Infarction Risk in An Iranian Population

Background: Oxidative stress induced by superoxide anion plays critical roles in the pathogenesis of coronary artery disease (CAD) and hence acute myocardial infarction (AMI). The major source of superoxide production in vascular smooth muscle and endothelial cells is the NADPH oxidase complex. An essential component of this complex is p22phox, that is encoded by the cytochrome b-245, alpha polypeptide (CYBA) gene. The aim of this study was to investigate the association of CYBA variants (rs1049255 and rs4673) and premature acute myocardial infarction risk in an Iranian population. Methods: The study population consisted of 158 patients under the age of 50 years, with a diagnosis of premature AMI, and 168 age-matched controls with normal coronary angiograms. Genotyping of the polymorphisms was performed by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: There was no association between the genotypes and allele frequencies of rs4673 polymorphism and premature acute myocardial infarction (P>0.05). A significant statistical association was observed between the genotypes distribution of rs1049255 polymorphism and AMI risk (P=0.037). Furthermore, the distribution of AA+AG/GG genotypes was found to be statistically significant between the two groups (P=0.011). Conclusions: Our findings indicated that rs1049255 but not rs4673 polymorphism is associated with premature AMI

On-Site Detection of Carcinoembryonic Antigen in Human Serum

Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.</jats:p