14 research outputs found
Optical frequency comb transfer through 820-m-scale atmospheric turbulence for low-noise radiofrequency distribution
Two classes of bacterial IMPDHs according to their quaternary structures and catalytic properties.
Inosine-5'-monophosphate dehydrogenase (IMPDH) occupies a key position in purine nucleotide metabolism. In this study, we have performed the biochemical and physico-chemical characterization of eight bacterial IMPDHs, among which six were totally unexplored. This study led to a classification of bacterial IMPDHs according to the regulation of their catalytic properties and their quaternary structures. Class I IMPDHs are cooperative enzymes for IMP, which are activated by MgATP and are octameric in all tested conditions. On the other hand, class II IMPDHs behave as Michaelis-Menten enzymes for both substrates and are tetramers in their apo state or in the presence of IMP, which are shifted to octamers in the presence of NAD or MgATP. Our work provides new insights into the IMPDH functional regulation and a model for the quaternary structure modulation is proposed
Helicobacter pylori Relies Primarily on the Purine Salvage Pathway for Purine Nucleotide Biosynthesis
Diacylglycerol O-Acyltransferase Type-1 Synthesizes Retinyl Esters in the Retina and Retinal Pigment Epithelium
Two Classes of Bacterial IMPDHs according to Their Quaternary Structures and Catalytic Properties
MOESM1 of Development of a production chain from vegetable biowaste to platform chemicals
Additional file 1: Table S1. Primer sequences and amplicon size for the 16S Illumina MiSeq