Additional file 1: Table S1. Primer sequences and amplicon size for the 16S Illumina MiSeq

A Ghimire

A Lemmer

A Oren

A Prokhorova

A Schievano

A Schlüter

A Wang

A Xia

AE Rotaru

AH Förster

Andreas Lemmer

Annemarie Schmidt

B Buchfink

B Liang

BE Logan

BR Dhar

C Barca

C Barelli

C Chassard

C Travaglini-Allocatelli

Christian Jonas Lapp

CO Tuck

D Rivière

D Zitomer

Daniel Siebert

DR Bond

E Kipf

E Lalaurette

F Mosbæk

Florencia Saravia

FM Simonte

G Zellner

Gunnar Sturm

H Du

H Kobayashi

H Sträuber

Harald Horn

HJ Flint

IH Franke-Whittle

J Gustavsson

J Ryckeboer

J Wenzel

J-R Bastidas-Oyanedel

Johannes Gescher

K Dolch

K Sturm-Richter

KJ Chae

KP Scott

M Balat

M Gollapalli

MJ McInerney

N Müller

P Kjeldsen

P Weiland

Padma Priya Ravi

PC Chiu

PP Ravi

Q Lu

R Valcheva

SS Dhiman

T Baba

T Bursac

T Chookaew

T Narihiro

X-H Li

Y Chen

Y Xia

Z Zhao

Microbial Cell Factories

Annemarie Schmidt (5393417)

Gunnar Sturm (5393432)

Christian Lapp (5393420)

Daniel Siebert (1591852)

Florencia Saravia (5393423)

Harald Horn (1734085)

Padma Ravi (5393429)

Andreas Lemmer (5393426)

Johannes Gescher (1712305)

FigShare

MOESM1 of Development of a production chain from vegetable biowaste to platform chemicals

Schmidt, Annemarie

Sturm, Gunnar

Lapp, Christian Jonas

Siebert, Daniel

Saravia, Florencia

Horn, Harald

Ravi, Padma Priya

Lemmer, Andreas

Gescher, Johannes

KITopen

Development of a production chain from vegetable biowaste to platform chemicals

Crossref

Abstract Background A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt to design a biotechnological cascade for the conversion of vegetable waste into acetoin and electrical energy. Results A vegetable waste dark fermentation effluent containing mainly acetate, butyrate and propionate was oxidized in a bioelectrochemical system. The achieved average current at a constant anode potential of 0 mV against standard hydrogen electrode was 177.5 ± 52.5 µA/cm2. During this step, acetate and butyrate were removed from the effluent while propionate was the major remaining component of the total organic carbon content comprising on average 75.6%. The key players with regard to carbon oxidation and electrode reduction were revealed using amplicon sequencing and metatranscriptomic analysis. Using nanofiltration, it was possible to concentrate the propionate in the effluent. The effluent was revealed to be a suitable medium for biotechnological production strains. As a proof of principle, the propionate in the effluent of the bioelectrochemical system was converted into the platform chemical acetoin with a carbon recovery of 86%. Conclusions To the best of our knowledge this is the first report on a full biotechnological production chain leading from vegetable waste to the production of a single valuable platform chemical that integrates carbon elimination steps leading to the production of the valuable side product electrical energy

Directory of Open Access Journals

https://publikationen.bibliothek.kit.edu/1000084121/14983541

MOESM1 of Development of a production chain from vegetable biowaste to platform chemicals

Abstract

Similar works

Full text

Available Versions

FigShare

KITopen

Crossref

Directory of Open Access Journals